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学科主题临床医学
The clinical significance of Psoriasin for non-small cell lung cancer patients and its biological impact on lung cancer cell functions
Hu, Mu1,2,3,4; Ye, Lin1,2; Ruge, Fiona1,2; Zhi, Xiuyi3,4; Zhang, Lijian5,6; Jiang, Wen G.1,2
关键词Psoriasin S100a7 Lung Cancer Adhesion And Invasion
刊名BMC CANCER
2012-12-10
DOI10.1186/1471-2407-12-588
12
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Oncology
资助者Cardiff University China Medical Scholarship ; Albert Hung Foundation ; Cancer Research Wales ; Cardiff University China Medical Scholarship ; Albert Hung Foundation ; Cancer Research Wales
研究领域[WOS]Oncology
关键词[WOS]BREAST-CANCER ; IN-VITRO ; EXPRESSION ; S100A7 ; CARCINOMA ; PROTEIN ; ADENOCARCINOMA ; VALIDATION ; MIGRATION ; SURVIVAL
英文摘要

Background: Psoriasin (S100A7) is a member of the S100 gene family. Alteration of Psoriasin expression has previously been reported to play an important role in cancer aggressive behaviour. The current study sought to investigate the level of Psoriasin expression at the mRNA level in a cohort of patients with non-small cell lung cancer (NSCLC), the association with clinical implication and outcomes, and the molecular and cellular impact of the protein on lung cancer cells.

Methods: Fresh frozen NSCLC cell carcinoma tissues, along with matched normal tissues were obtained from 83 NSCLC patients who received curative resection from January 2003 to December 2011. The expression of Psoriasin in the NSCLC specimens was assessed using both quantitative real time PCR (QPCR) and immunochemical staining. Knockdown and forced expression of Psoriasin in NSCLC cell lines were carried out using constructed plasmid vectors carrying either ribozyme transgenes targeting human Psoriasin or full-length coding sequence, respectively. The effect of Psoriasin on the functions of NSCLC cells was determined using a variety of in vitro cell function assays.

Results: Higher mRNA levels of Psoriasin were observed in tumour tissues when compared to both the paired normal background tissues and none paired normal tissues (p = 0.0251 and 0.0195). The mRNA level of Psoriasin was found to be higher in the squamous carcinoma (P = 0.035). Higher Psoriasin expression is associated with poor prognosis. The cell function tests had supportive results to the clinical findings. Over-expression of Posriasin in lung cancer cells (SK-MES-1) resulted in an increase in in vitro growth and invasiveness. In contrast, Psoriasin knockdown suppressed cell growth and invasion (P<0.05), but increased cell adhesion (P<0.05).

Conclusions: Psoriasin expression is increased in lung cancer, more specifically in lung squamous carcinoma compared with adenocarcinoma, and is associated with poor prognosis. Psoriasin plays crucial roles in regulating the growth and invasion of lung cancer cells.

语种英语
资助者Cardiff University China Medical Scholarship ; Albert Hung Foundation ; Cancer Research Wales ; Cardiff University China Medical Scholarship ; Albert Hung Foundation ; Cancer Research Wales
WOS记录号WOS:000313270600001
引用统计
被引频次:6[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/50685
专题北京大学临床肿瘤学院_胸外科
作者单位1.Cardiff Univ Capital Med Univ Joint Ctr Biomed Re, Cardiff, S Glam, Wales
2.Cardiff Univ, Sch Med, Metastasis & Angiogenesis Res Grp, Cardiff CF14 4XN, S Glam, Wales
3.Beijing Canc Hosp & Inst, Beijing 100142, Peoples R China
4.Capital Med Univ, Xuanwu Hosp, Lung Canc Lab, Beijing 100053, Peoples R China
5.Capital Med Univ, Xuanwu Hosp, Dept Thorac Surg, Beijing 100053, Peoples R China
6.Peking Univ, Sch Oncol, Dept Thorac Surg, Beijing 100142, Peoples R China
推荐引用方式
GB/T 7714
Hu, Mu,Ye, Lin,Ruge, Fiona,et al. The clinical significance of Psoriasin for non-small cell lung cancer patients and its biological impact on lung cancer cell functions[J]. BMC CANCER,2012,12.
APA Hu, Mu,Ye, Lin,Ruge, Fiona,Zhi, Xiuyi,Zhang, Lijian,&Jiang, Wen G..(2012).The clinical significance of Psoriasin for non-small cell lung cancer patients and its biological impact on lung cancer cell functions.BMC CANCER,12.
MLA Hu, Mu,et al."The clinical significance of Psoriasin for non-small cell lung cancer patients and its biological impact on lung cancer cell functions".BMC CANCER 12(2012).
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