北京大学医学部机构知识库
Advanced  
IR@PKUHSC  > 北京大学临床肿瘤学院  > 胸外科  > 期刊论文
学科主题: 临床医学
题名:
The clinical significance of Psoriasin for non-small cell lung cancer patients and its biological impact on lung cancer cell functions
作者: Hu, Mu1,2,3,4; Ye, Lin1,2; Ruge, Fiona1,2; Zhi, Xiuyi3,4; Zhang, Lijian5,6; Jiang, Wen G.1,2
关键词: Psoriasin ; S100A7 ; Lung cancer ; Adhesion and invasion
刊名: BMC CANCER
发表日期: 2012-12-10
DOI: 10.1186/1471-2407-12-588
卷: 12
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Oncology
研究领域[WOS]: Oncology
关键词[WOS]: BREAST-CANCER ; IN-VITRO ; EXPRESSION ; S100A7 ; CARCINOMA ; PROTEIN ; ADENOCARCINOMA ; VALIDATION ; MIGRATION ; SURVIVAL
英文摘要:

Background: Psoriasin (S100A7) is a member of the S100 gene family. Alteration of Psoriasin expression has previously been reported to play an important role in cancer aggressive behaviour. The current study sought to investigate the level of Psoriasin expression at the mRNA level in a cohort of patients with non-small cell lung cancer (NSCLC), the association with clinical implication and outcomes, and the molecular and cellular impact of the protein on lung cancer cells.

Methods: Fresh frozen NSCLC cell carcinoma tissues, along with matched normal tissues were obtained from 83 NSCLC patients who received curative resection from January 2003 to December 2011. The expression of Psoriasin in the NSCLC specimens was assessed using both quantitative real time PCR (QPCR) and immunochemical staining. Knockdown and forced expression of Psoriasin in NSCLC cell lines were carried out using constructed plasmid vectors carrying either ribozyme transgenes targeting human Psoriasin or full-length coding sequence, respectively. The effect of Psoriasin on the functions of NSCLC cells was determined using a variety of in vitro cell function assays.

Results: Higher mRNA levels of Psoriasin were observed in tumour tissues when compared to both the paired normal background tissues and none paired normal tissues (p = 0.0251 and 0.0195). The mRNA level of Psoriasin was found to be higher in the squamous carcinoma (P = 0.035). Higher Psoriasin expression is associated with poor prognosis. The cell function tests had supportive results to the clinical findings. Over-expression of Posriasin in lung cancer cells (SK-MES-1) resulted in an increase in in vitro growth and invasiveness. In contrast, Psoriasin knockdown suppressed cell growth and invasion (P<0.05), but increased cell adhesion (P<0.05).

Conclusions: Psoriasin expression is increased in lung cancer, more specifically in lung squamous carcinoma compared with adenocarcinoma, and is associated with poor prognosis. Psoriasin plays crucial roles in regulating the growth and invasion of lung cancer cells.

语种: 英语
项目资助者: Cardiff University China Medical Scholarship ; Albert Hung Foundation ; Cancer Research Wales
WOS记录号: WOS:000313270600001
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/50685
Appears in Collections:北京大学临床肿瘤学院_胸外科_期刊论文

Files in This Item:

There are no files associated with this item.


作者单位: 1.Cardiff Univ Capital Med Univ Joint Ctr Biomed Re, Cardiff, S Glam, Wales
2.Cardiff Univ, Sch Med, Metastasis & Angiogenesis Res Grp, Cardiff CF14 4XN, S Glam, Wales
3.Beijing Canc Hosp & Inst, Beijing 100142, Peoples R China
4.Capital Med Univ, Xuanwu Hosp, Lung Canc Lab, Beijing 100053, Peoples R China
5.Capital Med Univ, Xuanwu Hosp, Dept Thorac Surg, Beijing 100053, Peoples R China
6.Peking Univ, Sch Oncol, Dept Thorac Surg, Beijing 100142, Peoples R China

Recommended Citation:
Hu, Mu,Ye, Lin,Ruge, Fiona,et al. The clinical significance of Psoriasin for non-small cell lung cancer patients and its biological impact on lung cancer cell functions[J]. BMC CANCER,2012,12.
Service
Recommend this item
Sava as my favorate item
Show this item's statistics
Export Endnote File
Google Scholar
Similar articles in Google Scholar
[Hu, Mu]'s Articles
[Ye, Lin]'s Articles
[Ruge, Fiona]'s Articles
CSDL cross search
Similar articles in CSDL Cross Search
[Hu, Mu]‘s Articles
[Ye, Lin]‘s Articles
[Ruge, Fiona]‘s Articles
Related Copyright Policies
Null
Social Bookmarking
Add to CiteULike Add to Connotea Add to Del.icio.us Add to Digg Add to Reddit
所有评论 (0)
暂无评论
 
评注功能仅针对注册用户开放,请您登录
您对该条目有什么异议,请填写以下表单,管理员会尽快联系您。
内 容:
Email:  *
单位:
验证码:   刷新
您在IR的使用过程中有什么好的想法或者建议可以反馈给我们。
标 题:
 *
内 容:
Email:  *
验证码:   刷新

Items in IR are protected by copyright, with all rights reserved, unless otherwise indicated.

 

 

Valid XHTML 1.0!
Copyright © 2007-2017  北京大学医学部 - Feedback
Powered by CSpace