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学科主题: 基础医学
题名:
Caffeine induced Ca2+ release and capacitative Ca2+ entry in human embryonic kidney (HEK293) cells
作者: Luo, DL1; Sun, H1; Xiao, RP1; Han, Q1
关键词: ryanodine receptor ; HEK293 cells ; Ca2+ release ; capacitative Ca2+ entry
刊名: EUROPEAN JOURNAL OF PHARMACOLOGY
发表日期: 2005-02-21
DOI: 10.1016/j.ejphar.2004.12.038
卷: 509, 期:2-3, 页:109-115
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Pharmacology & Pharmacy
研究领域[WOS]: Pharmacology & Pharmacy
关键词[WOS]: RYANODINE RECEPTOR ; SIGNALING PATHWAYS ; HEK-293 CELLS ; EXPRESSION ; STORE ; CALCIUM ; SPECIFICITY ; CHANNELS ; ISOFORMS
英文摘要:

The potential role of endogenous ryanodine receptor (RyR) in modulating Ca2+ handling in HEK293 cells is controversial. Using Fura2/AM, here we provide evidence that caffeine can induce Ca2+ release from inositol 1,4,5-trisphosphate receptor-sensitive stores and Ca2+ entry in early passage numbers of HEK293 cells, but not in late passage ones. Ryanodine blocks caffeine-mediated effect, whereas 4-chloro-m-cresol can mimic these effects. In contrast, an increase in cyclic AMP or activation of voltage-dependent Ca2+ channels does not induce detectable alteration in intracellular Ca2+. Importantly, inummoblotting and staining have revealed that endogenous RyR expression is more abundant in the early than in the late passage cells. Additionally, similar to carbachol, Ca2+ entry in response to caffeine is blocked by capacitative Ca2+ entry inhibitors. These results indicate that the endogenous RyR in HEK293 cells can function as Ca2+ release channels and mediate capacitative Ca2+ entry, but they may be reduced due to cell passage. (C) 2005 Elsevier B.V. All rights reserved.

语种: 英语
WOS记录号: WOS:000227466100003
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/50706
Appears in Collections:基础医学院_心血管所_期刊论文

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作者单位: 1.Peking Univ, Hlth Sci Ctr, Inst Cardiovasc Sci, Beijing 100083, Peoples R China
2.NIA, Cardiovasc Sci Lab, NIH, Baltimore, MD 21224 USA

Recommended Citation:
Luo, DL,Sun, H,Xiao, RP,et al. Caffeine induced Ca2+ release and capacitative Ca2+ entry in human embryonic kidney (HEK293) cells[J]. EUROPEAN JOURNAL OF PHARMACOLOGY,2005,509(2-3):109-115.
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