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学科主题: 临床医学
题名:
Autophagic deficiency is related to steroidogenic decline in aged rat Leydig cells
作者: Li, Wei-Ren1; Chen, Liang2; Chang, Zhi-Jie3; Xin, Hua1; Liu, Tao1; Zhang, Yan-Quan3; Li, Guang-Yong1; Zhou, Feng1; Gong, Yan-Qing1; Gao, Zhe-Zhu1; Xin, Zhong-Cheng1
关键词: ageing ; autophagy ; late onset hypogonadism ; Leydig cell ; reactive oxygen species ; testosterone
刊名: ASIAN JOURNAL OF ANDROLOGY
发表日期: 2011-11-01
DOI: 10.1038/aja.2011.85
卷: 13, 期:6, 页:881-888
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Andrology ; Urology & Nephrology
研究领域[WOS]: Endocrinology & Metabolism ; Urology & Nephrology
关键词[WOS]: LATE-ONSET HYPOGONADISM ; REGULATORY STAR PROTEIN ; CALORIE RESTRICTION ; TESTOSTERONE PRODUCTION ; MITOCHONDRIA ; MUSCLE ; RECOMMENDATIONS ; DYSFUNCTION ; INHIBITION ; LONGEVITY
英文摘要:

Late-onset hypogonadism (LOH) is closely related to secondary androgen deficiency in aged males, but the mechanism remains unclear. In this study, we found that reduced testosterone production in aged rat Leydig cells is associated with decreased autophagic activity. Primary rat Leydig cells and the TM3 mouse Leydig cell line were used to study the effect of autophagic deficiency on Leydig cell testosterone production. In Leydig cells from young and aged rats, treatment with wortmannin, an autophagy inhibitor, inhibited luteinising hormone (LH)-stimulated steroidogenic acute regulatory (StAR) protein expression and decreased testosterone production. In contrast, treatment with rapamycin, an autophagy activator, enhanced LH-stimulated steroidogenesis in Leydig cells from aged, but not young, rats. Intracellular reactive oxygen species (ROS) levels were increased in both young and aged Leydig cells treated with wortmannin but decreased only in aged Leydig cells treated with rapamycin. Furthermore, an increased level of ROS, induced by H(2)O(2), resulted in LH-stimulated steroidogenic inhibition. Finally, knockdown of Beclin 1 decreased LH-stimulated StAR expression and testosterone production in TM3 mouse Leydig cells, which were associated with increased intracellular ROS level. These results suggested that autophagic deficiency is related to steroidogenic decline in aged rat Leydig cells, which might be influenced by intracellular ROS levels. Asian Journal of Andrology (2011) 13, 881-888; doi:10.1038/aja.2011.85; published online 8 August 2011

语种: 英语
所属项目编号: 30772285 ; 30901492
项目资助者: National Natural Science Foundation of China
WOS记录号: WOS:000296752900026
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/50712
Appears in Collections:北京大学第一临床医学院_男科中心_期刊论文

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作者单位: 1.Peking Univ, Hosp 1, Androl Ctr, Beijing 100034, Peoples R China
2.China Meitan Gen Hosp, Dept Urol, Beijing 100028, Peoples R China
3.Tsinghua Univ, Sch Med, Dept Biol Sci & Biotechnol, Beijing 100084, Peoples R China

Recommended Citation:
Li, Wei-Ren,Chen, Liang,Chang, Zhi-Jie,et al. Autophagic deficiency is related to steroidogenic decline in aged rat Leydig cells[J]. ASIAN JOURNAL OF ANDROLOGY,2011,13(6):881-888.
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