IR@PKUHSC  > 北京大学第一临床医学院  > 男科中心
学科主题临床医学
Autophagic deficiency is related to steroidogenic decline in aged rat Leydig cells
Li, Wei-Ren1; Chen, Liang2; Chang, Zhi-Jie3; Xin, Hua1; Liu, Tao1; Zhang, Yan-Quan3; Li, Guang-Yong1; Zhou, Feng1; Gong, Yan-Qing1; Gao, Zhe-Zhu1; Xin, Zhong-Cheng1
关键词Ageing Autophagy Late Onset Hypogonadism Leydig Cell Reactive Oxygen Species Testosterone
刊名ASIAN JOURNAL OF ANDROLOGY
2011-11-01
DOI10.1038/aja.2011.85
13期:6页:881-888
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Andrology ; Urology & Nephrology
资助者National Natural Science Foundation of China ; National Natural Science Foundation of China
研究领域[WOS]Endocrinology & Metabolism ; Urology & Nephrology
关键词[WOS]LATE-ONSET HYPOGONADISM ; REGULATORY STAR PROTEIN ; CALORIE RESTRICTION ; TESTOSTERONE PRODUCTION ; MITOCHONDRIA ; MUSCLE ; RECOMMENDATIONS ; DYSFUNCTION ; INHIBITION ; LONGEVITY
英文摘要

Late-onset hypogonadism (LOH) is closely related to secondary androgen deficiency in aged males, but the mechanism remains unclear. In this study, we found that reduced testosterone production in aged rat Leydig cells is associated with decreased autophagic activity. Primary rat Leydig cells and the TM3 mouse Leydig cell line were used to study the effect of autophagic deficiency on Leydig cell testosterone production. In Leydig cells from young and aged rats, treatment with wortmannin, an autophagy inhibitor, inhibited luteinising hormone (LH)-stimulated steroidogenic acute regulatory (StAR) protein expression and decreased testosterone production. In contrast, treatment with rapamycin, an autophagy activator, enhanced LH-stimulated steroidogenesis in Leydig cells from aged, but not young, rats. Intracellular reactive oxygen species (ROS) levels were increased in both young and aged Leydig cells treated with wortmannin but decreased only in aged Leydig cells treated with rapamycin. Furthermore, an increased level of ROS, induced by H(2)O(2), resulted in LH-stimulated steroidogenic inhibition. Finally, knockdown of Beclin 1 decreased LH-stimulated StAR expression and testosterone production in TM3 mouse Leydig cells, which were associated with increased intracellular ROS level. These results suggested that autophagic deficiency is related to steroidogenic decline in aged rat Leydig cells, which might be influenced by intracellular ROS levels. Asian Journal of Andrology (2011) 13, 881-888; doi:10.1038/aja.2011.85; published online 8 August 2011

语种英语
所属项目编号30772285 ; 30901492
资助者National Natural Science Foundation of China ; National Natural Science Foundation of China
WOS记录号WOS:000296752900026
Citation statistics
Cited Times:17[WOS]   [WOS Record]     [Related Records in WOS]
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/50712
Collection北京大学第一临床医学院_男科中心
作者单位1.Peking Univ, Hosp 1, Androl Ctr, Beijing 100034, Peoples R China
2.China Meitan Gen Hosp, Dept Urol, Beijing 100028, Peoples R China
3.Tsinghua Univ, Sch Med, Dept Biol Sci & Biotechnol, Beijing 100084, Peoples R China
Recommended Citation
GB/T 7714
Li, Wei-Ren,Chen, Liang,Chang, Zhi-Jie,et al. Autophagic deficiency is related to steroidogenic decline in aged rat Leydig cells[J]. ASIAN JOURNAL OF ANDROLOGY,2011,13(6):881-888.
APA Li, Wei-Ren.,Chen, Liang.,Chang, Zhi-Jie.,Xin, Hua.,Liu, Tao.,...&Xin, Zhong-Cheng.(2011).Autophagic deficiency is related to steroidogenic decline in aged rat Leydig cells.ASIAN JOURNAL OF ANDROLOGY,13(6),881-888.
MLA Li, Wei-Ren,et al."Autophagic deficiency is related to steroidogenic decline in aged rat Leydig cells".ASIAN JOURNAL OF ANDROLOGY 13.6(2011):881-888.
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