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The Role of Survivin in Podocyte Injury Induced by Puromycin Aminonucleoside
Li, Xuejuan; Zhang, Xiaoyan; Li, Xiaoyan; Ding, Fangrui; Ding, Jie
关键词Survivin Podocyte Apoptosis Nephropathy Cyto-protection
刊名INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
2014-04-01
DOI10.3390/ijms15046657
15期:4页:6657-6673
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Biochemistry & Molecular Biology ; Chemistry, Multidisciplinary
资助者National Basic Research Program of China (973 Program) ; National Nature Science Foundation of China ; Nature Science Foundation of Beijing ; National Basic Research Program of China (973 Program) ; National Nature Science Foundation of China ; Nature Science Foundation of Beijing
研究领域[WOS]Biochemistry & Molecular Biology ; Chemistry
关键词[WOS]FOCAL SEGMENTAL GLOMERULOSCLEROSIS ; GLOMERULAR EPITHELIAL-CELLS ; APOPTOSIS PROTEIN ; BREAST-CANCER ; IN-VITRO ; EXPRESSION ; INHIBITOR ; GENE ; NEPHROSIS ; ACTIVATION
英文摘要

Objective: Survivin is a member of the inhibitor of apoptosis protein family, which uniquely promotes mitosis and regulates apoptosis in cancer cells. Recent studies have demonstrated that survivin also expresses in several normal adult cells. In the present study, we aimed to investigate the function of survivin in the terminally differentiated epithelial cells, podocytes. Methods: Survivin expression and location were detected by Quantitative Real-Time PCR, western blot and fluorescence confocal microscopy methods in normal and injured mouse podocytes. Cyto-protection function of survivin was also studied in cultured podocyte injured by puromycin aminonucleoside (PAN), transfected with survivin siRNA to down-regulate survivin expression, or with survivin plasmid to transiently over-express survivin. Results: In podocytes, PAN stimulated expressions of survivin and the apoptosis related molecule caspase 3. Knockdown of survivin expression by siRNA increased the activation of caspase 3, induced podocyte apoptosis and remarkable rearrangement of actin cytoskeleton. Moreover, over-expression of survivin inhibited PAN-induced podocyte apoptosis and cytoskeleton rearrangement. Conclusion: Our data provides the evidence that survivin plays an important role in protecting podocytes from apoptosis induced by PAN. The mechanism of survivin related anti-apoptosis may, at least partially, be through the activation of caspase 3.

语种英语
所属项目编号2012CB517700 ; 30830105 ; 81170657 ; 7072080
资助者National Basic Research Program of China (973 Program) ; National Nature Science Foundation of China ; Nature Science Foundation of Beijing ; National Basic Research Program of China (973 Program) ; National Nature Science Foundation of China ; Nature Science Foundation of Beijing
WOS记录号WOS:000336841200097
引用统计
被引频次:6[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/50714
专题北京大学第一临床医学院_儿科
作者单位Peking Univ, Dept Pediat, Hosp 1, Beijing 100034, Peoples R China
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GB/T 7714
Li, Xuejuan,Zhang, Xiaoyan,Li, Xiaoyan,et al. The Role of Survivin in Podocyte Injury Induced by Puromycin Aminonucleoside[J]. INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES,2014,15(4):6657-6673.
APA Li, Xuejuan,Zhang, Xiaoyan,Li, Xiaoyan,Ding, Fangrui,&Ding, Jie.(2014).The Role of Survivin in Podocyte Injury Induced by Puromycin Aminonucleoside.INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES,15(4),6657-6673.
MLA Li, Xuejuan,et al."The Role of Survivin in Podocyte Injury Induced by Puromycin Aminonucleoside".INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES 15.4(2014):6657-6673.
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