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学科主题: 基础医学
题名:
Isolation and functional analysis of autoreactive T cells from BXSB mice with murine lupus
作者: Han, SH; Li, B; Chen, YT; Gao, XM
关键词: autoreactive T cells ; BXSB ; lupus
刊名: JOURNAL OF AUTOIMMUNITY
发表日期: 2002-08-01
DOI: 10.1006/jaut.2002.0600
卷: 19, 期:1-2, 页:45-54
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Immunology
研究领域[WOS]: Immunology
关键词[WOS]: SUSCEPTIBILITY LOCI MAP ; AUTOIMMUNE-DISEASE ; B-CELL ; AUTOANTIBODY PRODUCTION ; MONOCLONAL-ANTIBODY ; ERYTHEMATOSUS ; IDENTIFICATION ; CLONES ; CHROMOSOME-1 ; NUCLEOSOME
英文摘要:

CD4(+) T helper cells play a pivotal role in the pathogenesis of SLE, although the mechanism is still unclear. The present study was designed to isolate and characterize autoreactive T lymphocytes from BXSB mice, a mouse model for human SLE. Splenocytes from 6-month-old male BXSB mice with murine lupus were repeatedly stimulated in vitro with irradiated syngeneic B cells in the presence of recombinant IL-2, resulting in six autoreactive T-cell lines and two T-cell clones. TCR analysis showed that, one of the T-cell lines, ATL1, was almost clonal, as a Vbeta2.1-Jbeta2, a Valpha5.1-Jalpha15 and a Valpha10.1-Jalpha15 chains were predominantly expressed in this line. The two clones derived from ATL1 turned out to be sister clones, using the TCR Vbeta2.1-Jbeta2 and Valpha10.1-Jalpha15 chains. ATL1 cells proliferated in response to stimulation of syngeneic and H-2-matched allogeneic B cells and secreted IFN-gamma. Monoclonal Ab against CD4 and CD28 inhibited the proliferative response of ATL1 for syngeneic B cells. Interestingly, ATL1 did not respond to BXSB spleen or peritoneal macrophages, suggesting that B cells were able to either express accessory molecules necessary for T-cell triggering or present cryptic epitopes recognized by the autoreactive T cells. Moreover, ATL1 was able to help BXSB, but not C57BL/6, B cells producing IgG and TgM Abs against dsDNA and histone in vitro. Passive transfer of viable ATL1 cells into young female BXSB mice significantly accelerated the production of autoantibodies. Possible mechanisms of interaction between ATL1 and lupus B cells are further discussed. (C) 2002 Elsevier Science Ltd. All rights reserved.

语种: 英语
WOS记录号: WOS:000178573000005
Citation statistics:
内容类型: 期刊论文
版本: 出版稿
URI标识: http://ir.bjmu.edu.cn/handle/400002259/50715
Appears in Collections:基础医学院_免疫学系_期刊论文

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作者单位: Peking Univ, Ctr Hlth Sci, Dept Immunol, Beijing 100083, Peoples R China

Recommended Citation:
Han, SH,Li, B,Chen, YT,et al. Isolation and functional analysis of autoreactive T cells from BXSB mice with murine lupus[J]. JOURNAL OF AUTOIMMUNITY,2002,19(1-2):45-54.
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