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学科主题: 临床医学
题名:
TRPC6 Up-Regulation in Ang II-Induced Podocyte Apoptosis Might Result from ERK Activation and NF-kappa B Translocation
作者: Zhang, Han; Ding, Jie; Fan, Qingfeng; Liu, Shufang
关键词: podocyte apoptosis ; angiotensin II ; TRPC6 ; calcium ; ERK ; NF-kappa B
刊名: EXPERIMENTAL BIOLOGY AND MEDICINE
发表日期: 2009-09-01
DOI: 10.3181/0901-RM-11
卷: 234, 期:9, 页:1029-1036
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Medicine, Research & Experimental
研究领域[WOS]: Research & Experimental Medicine
关键词[WOS]: FOCAL SEGMENTAL GLOMERULOSCLEROSIS ; EPITHELIAL-CELLS ; ANGIOTENSIN ; DISEASE ; KIDNEY ; BIOLOGY
英文摘要:

Angiotensin II (Ang II) has been recognized as an apoptosis inducer in podocytes, but the mechanism of apoptosis induced by Ang II is unclear. Transient receptor potential cation channel 6 (TRPC6) is a calcium channel located in podocyte membrane. The present study evaluated the alteration of TRPC6 expression and the Ca(2+) influx involved in Ang II-induced podocyte apoptosis. The possible pathways related to TRPC6 in Ang II-induced podocyte apoptosis were also investigated. The apoptosis of mouse podocytes (MPC5) was induced by Ang II. The protein level of TRPC6 was increased markedly in response to Ang II stimulation, and the intracellular Ca(2+) concentration was elevated. By transfection with TRPC6 siRNA, Ang II-induced podocyte apoptosis and the transient Ca(2+) influx were inhibited. Treated with extracellular signal-regulated kinase (ERK) pathway specific inhibitor U0126 or nuclear factor-kappa B (NF-kappa B) pathway specific inhibitor ammonium pyrrolidinedithiocarbamate (PDTC) and Ang II, respectively in podocytes, not only was the TRPC6 up-regulation reduced, but the podocyte apoptosis was also decreased. Moreover, the translocation of NF-kappa B in nucleus resulted from Ang II was reduced by treatment with U0126. In conclusion, the enhancement expression of TRPC6 as well as the increased Ca(2+) influx mediated by TRPC6 channels contributed to the podocyte apoptosis. The activation of ERK pathway and subsequent translocation of NF-kappa B was possibly necessary for the up-regulation TRPC6 induced by Ang It. Exp Biol Med 234:1029-1036, 2009

语种: 英语
所属项目编号: 30170992 ; 30672259 ; 7072080 ; 20070001764
项目资助者: National Nature Science Foundation of China ; Nature Science Foundation of Beijing ; Chinese Ministry of Education
WOS记录号: WOS:000269966600004
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/50717
Appears in Collections:北京大学第一临床医学院_儿科_期刊论文

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作者单位: Peking Univ, Dept Pediat, Hosp 1, Beijing 100034, Peoples R China

Recommended Citation:
Zhang, Han,Ding, Jie,Fan, Qingfeng,et al. TRPC6 Up-Regulation in Ang II-Induced Podocyte Apoptosis Might Result from ERK Activation and NF-kappa B Translocation[J]. EXPERIMENTAL BIOLOGY AND MEDICINE,2009,234(9):1029-1036.
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