IR@PKUHSC  > 北京大学第三临床医学院  > 眼科
学科主题临床医学
The beta-catenin/Tcf4/survivin signaling maintains a less differentiated phenotype and high proliferative capacity of human corneal epithelial progenitor cells
Lu, Rong1,2; Bian, Fang1,3; Zhang, Xiaobo1,4; Qi, Hong1,5; Chuang, Eliseu Y.1; Pflugfelder, Stephen C.1; Li, De-Quan1
关键词Adult Stem Cell Stem Cell Niche Corneal Epithelium Beta-catenin Tcf4 Survivin
刊名INTERNATIONAL JOURNAL OF BIOCHEMISTRY & CELL BIOLOGY
2011-05-01
DOI10.1016/j.biocel.2011.01.018
43期:5页:751-759
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Biochemistry & Molecular Biology ; Cell Biology
研究领域[WOS]Biochemistry & Molecular Biology ; Cell Biology
英文摘要

It is clear that the microenvironment or niche plays an important role in determining the fate of stem cells: being stem cells or differentiated. However, the intrinsic pathways controlling the fate of adult stem cells in different niches are largely unknown. This study was to explore the role of beta-catenin/Tcf4/survivin signaling in determining the fate of human corneal epithelial stem cells in different media. We observed that the low calcium serum-free media, especially CnT-20, promoted proliferative capacity, colony forming efficiency and stem cell-like phenotype of human corneal epithelial cells (HCECs) when compared with the cells cultured in a high calcium serum-containing medium SHEM. Three key factors in Wnt signaling, beta-catenin, Tcf4 and survivin, were found to be expressed higher by HCECs grown in CnT-20 than those cultured in SHEM, as evaluated by real-time PCR, Western blotting and immunostaining. Transfection of siRNA-Tcf4 at 10-50 nM knocked down Tcf4, and also significantly suppressed its down stream molecule survivin at both mRNA and protein levels in HCECs. Furthermore, Tcf4 silencing significantly suppressed the proliferative capacity of HCECs, measured by WST-1 assay, compared with the control groups, untreated or transfected with non-coding sequence siRNA-fluorescein. These findings demonstrate that low calcium serum free media promote ex vivo expansion of corneal epithelial progenitor cells that retain a less differentiated phenotype and high proliferative capacity via beta-catenin/Tcf4/survivin signaling, a novel intrinsic pathway. This study may have high impact and clinic implication on the expansion of corneal epithelial stem cells in regenerative medicine, especially for ocular surface reconstruction. (C) 2011 Elsevier Ltd. All rights reserved.

语种英语
WOS记录号WOS:000290011900009
项目编号CDMRP PRMRP FY06 PR064719 ; EY11915 ; 30901634 ; 30872813 ; 9151008901000210
资助机构Department of Defense ; NEI NIH ; National Natural Science Foundation of China ; Natural Science Foundation of Guangdong Province ; Lions Foundation for Sight ; Research to Prevent Blindness ; Oshman Foundation ; William Stamps Farish Fund
引用统计
被引频次:24[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/50725
专题北京大学第三临床医学院_眼科
作者单位1.Baylor Coll Med, Ocular Surface Ctr, Cullen Eye Inst, Dept Ophthalmol, Houston, TX 77030 USA
2.Sun Yat Sen Univ, Zhongshan Ophthalm Ctr, State Key Lab Ophthalmol, Guangzhou 510275, Guangdong, Peoples R China
3.Huazhong Sci & Technol Univ, Union Hosp, Dept Ophthalmol, Tongji Med Coll, Wuhan, Peoples R China
4.Wenzhou Med Coll, Sch Ophthalmol & Optometry, Hosp Eye, Wenzhou, Peoples R China
5.Peking Univ, Hosp 3, Dept Ophthalmol, Beijing 100871, Peoples R China
推荐引用方式
GB/T 7714
Lu, Rong,Bian, Fang,Zhang, Xiaobo,et al. The beta-catenin/Tcf4/survivin signaling maintains a less differentiated phenotype and high proliferative capacity of human corneal epithelial progenitor cells[J]. INTERNATIONAL JOURNAL OF BIOCHEMISTRY & CELL BIOLOGY,2011,43(5):751-759.
APA Lu, Rong.,Bian, Fang.,Zhang, Xiaobo.,Qi, Hong.,Chuang, Eliseu Y..,...&Li, De-Quan.(2011).The beta-catenin/Tcf4/survivin signaling maintains a less differentiated phenotype and high proliferative capacity of human corneal epithelial progenitor cells.INTERNATIONAL JOURNAL OF BIOCHEMISTRY & CELL BIOLOGY,43(5),751-759.
MLA Lu, Rong,et al."The beta-catenin/Tcf4/survivin signaling maintains a less differentiated phenotype and high proliferative capacity of human corneal epithelial progenitor cells".INTERNATIONAL JOURNAL OF BIOCHEMISTRY & CELL BIOLOGY 43.5(2011):751-759.
条目包含的文件
条目无相关文件。
个性服务
推荐该条目
保存到收藏夹
查看访问统计
导出为Endnote文件
谷歌学术
谷歌学术中相似的文章
[Lu, Rong]的文章
[Bian, Fang]的文章
[Zhang, Xiaobo]的文章
百度学术
百度学术中相似的文章
[Lu, Rong]的文章
[Bian, Fang]的文章
[Zhang, Xiaobo]的文章
必应学术
必应学术中相似的文章
[Lu, Rong]的文章
[Bian, Fang]的文章
[Zhang, Xiaobo]的文章
相关权益政策
暂无数据
收藏/分享
所有评论 (0)
暂无评论
 

除非特别说明,本系统中所有内容都受版权保护,并保留所有权利。