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学科主题: 临床医学
题名:
Expression of MR-P14 gene is frequently down-regulated in Chinese human esophageal cancer
作者: Wang, J1; Cai, Y1; Xu, H1; Zhao, J1; Xu, X1; Han, YL1; Xu, ZX1; Chen, BS1; Hu, H1; Wu, M1; Wang, MR1
关键词: esophagus ; carcinoma ; MRP14 ; S100A9
刊名: CELL RESEARCH
发表日期: 2004-02-01
卷: 14, 期:1, 页:46-53
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Cell Biology
研究领域[WOS]: Cell Biology
关键词[WOS]: MRP14 ; CELLS ; CARCINOMA ; PROTEIN ; HETEROZYGOSITY ; LOCALIZATION ; MACROPHAGES ; MONOCYTES ; MORTALITY ; S100A8
英文摘要:

Migration inhibitory factor-related protein 14 (MRP14) is one of calcium-binding proteins, referred as S100A9. The heterodimeric molecule formed by MRP14 with its partner MRP8 (S100A8) is the major fatty acid carrier in neutrophils. The MRP8/14 complex has been also implicated in the intracellular transport of arachidonic acid and its precursors in keratinocytes. We show here the involvement of MRP14 in human esophageal cancer. In an initial study, mRNA differential display - reverse transcription polymerase chain reaction (DD-PCR) was performed with two esophageal carcinomas, one esophageal adenocarcinoma and matched normal adjacent mucosa. DD-PCR with the arbitrary primer OPA3 showed that one cDNA band was highly expressed in normal tissues, but disappeared or substantially decreased in tumor counterparts. It was later identified to be the 3′-end of migration inhibitory factor-related protein 14 (MRP14). Northern blotting, RT-PCR and Western blotting corroborated the down-regulation of MRP14 in 58/64 squamous cell carcinomas and 2/2 adenocarcinomas as compared with adjacent normal epithelia of the esophagus. MRP14 was undetectable in 3/3 esophageal-carcinoma cell lines. Immunochemistry demonstrated that expression of MRP14 was restricted to normal esophageal epithelia. No mutation was found in the genomic DNA of the MRP14 gene by PCR and directed DNA sequencing. Our finding suggested that the reduction of MRP14 expression is a frequent event in Chinese human esophageal cancer.

语种: 英语
WOS记录号: WOS:000220195100006
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/50727
Appears in Collections:北京大学临床肿瘤学院_胸外科_期刊论文

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作者单位: 1.Peking Univ, Chinese Acad Med Sci, Canc Inst Hosp, Natl Lab Mol Oncol,Med Sch, Beijing 100021, Peoples R China
2.Peking Univ, Sch Med, Dept Thorac Surg, Canc Inst Hosp, Beijing 100021, Peoples R China

Recommended Citation:
Wang, J,Cai, Y,Xu, H,et al. Expression of MR-P14 gene is frequently down-regulated in Chinese human esophageal cancer[J]. CELL RESEARCH,2004,14(1):46-53.
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