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Baicalein Reduces Inflammatory Process in a Rodent Model of Diabetic Retinopathy
Yang, Li-ping2; Sun, Hui-li1; Wu, Le-meng2; Guo, Xiu-juan2; Dou, Hong-liang2; Tso, Mark O. M.2,3; Zhao, Lin2; Li, Shun-min1
刊名INVESTIGATIVE OPHTHALMOLOGY & VISUAL SCIENCE
2009-05-01
DOI10.1167/iovs.08-2642
50期:5页:2319-2327
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Ophthalmology
研究领域[WOS]Ophthalmology
关键词[WOS]ENDOTHELIAL GROWTH-FACTOR ; BLOOD-RETINAL BARRIER ; MICROGLIAL ACTIVATION ; INHIBITION ; EXPRESSION ; RATS ; INTERLEUKIN-18 ; MICE ; EXTRACT ; BREAKDOWN
英文摘要

PURPOSE. This study was designed to elucidate the role of inflammatory process in diabetic retinopathy and to investigate the effect of baicalein treatment on diabetic rat.

METHODS. Retinal microglial cells were identified with CD11b antibody, and retinal Muller cells were identified with glial fibrillary acidic protein (GFAP). The gene expression of interleukin (IL)-18, tumor necrosis factor (TNF)-alpha, and IL-1 beta was examined by quantitative real-time PCR. The expression of GFAP and vascular endothelial growth factor ( VEGF) was examined by quantitative real-time PCR, immunohistochemistry, and Western blot analysis. Vascular permeability was measured in vivo by bovine serum albumin conjugated with FITC. Baicalein was given by oral administration (150 mg/kg/d) with an animal feeding needle beginning 5 days after streptozotocin (STZ) injection.

RESULTS. By 24 weeks after onset of diabetes, microglial cells were activated and proliferated, and Muller cells upregulated their GFAP and VEGF expression. Pro-inflammatory factors, including IL-18, TNF-alpha, and IL-1 beta, were significantly upregulated. Obvious vascular leakage and abnormality were demonstrated, and ganglion cell loss was significant. Baicalein treatment ameliorated diabetes-induced microglial activation and pro-inflammatory expression, reduced the GFAP and VEGF expression from Muller cells, and significantly reduced vascular abnormality and ganglion cell loss within the retina.

CONCLUSIONS. Inflammatory process, characterized by microglial activation and Muller cells dysfunction, was implicated in STZ-induced diabetic retinopathy. Baicalein treatment ameliorated inflammatory process, and therefore inhibited vascular abnormality and neuron loss in diabetic retinas. (Invest Ophthalmol Vis Sci. 2009;50:2319-2327) DOI:10.1167/iovs.08-2642

语种英语
WOS记录号WOS:000265451000047
引用统计
被引频次:72[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/50732
专题北京大学第三临床医学院_眼科
作者单位1.Guangzhou Univ Tradit Chinese Med, Shenzhen Affiliated Hosp, Dept Nephrol, Shenzhen 518033, Peoples R China
2.Peking Univ, Hosp 3, Ctr Eye, Beijing 100871, Peoples R China
3.Johns Hopkins Univ, Sch Med, Wilmer Eye Inst, Baltimore, MD 21205 USA
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GB/T 7714
Yang, Li-ping,Sun, Hui-li,Wu, Le-meng,et al. Baicalein Reduces Inflammatory Process in a Rodent Model of Diabetic Retinopathy[J]. INVESTIGATIVE OPHTHALMOLOGY & VISUAL SCIENCE,2009,50(5):2319-2327.
APA Yang, Li-ping.,Sun, Hui-li.,Wu, Le-meng.,Guo, Xiu-juan.,Dou, Hong-liang.,...&Li, Shun-min.(2009).Baicalein Reduces Inflammatory Process in a Rodent Model of Diabetic Retinopathy.INVESTIGATIVE OPHTHALMOLOGY & VISUAL SCIENCE,50(5),2319-2327.
MLA Yang, Li-ping,et al."Baicalein Reduces Inflammatory Process in a Rodent Model of Diabetic Retinopathy".INVESTIGATIVE OPHTHALMOLOGY & VISUAL SCIENCE 50.5(2009):2319-2327.
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