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学科主题基础医学
Effects of Lipid Composition and Phase on the Membrane Interaction of the Prion Peptide 106-126 Amide
Zhong, Jian1; Yang, Chunhui1; Zheng, Wenfu1; Huang, Lixin1; Hong, Yuankai1; Wang, Lijun1; Sha, Yinlin1
刊名BIOPHYSICAL JOURNAL
2009-06-03
DOI10.1016/j.bpj.2009.01.036
96期:11页:4610-4621
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Biophysics
研究领域[WOS]Biophysics
关键词[WOS]ATOMIC-FORCE MICROSCOPY ; MODEL MEMBRANES ; TRITON X-100 ; ANTIMICROBIAL PEPTIDES ; ALZHEIMERS-DISEASE ; SUPPORTED BILAYERS ; PROTEIN-FRAGMENT ; AMYLOID PEPTIDE ; CELL-MEMBRANES ; RAFT FORMATION
英文摘要

Lipid rafts are specialized liquid-ordered (L-o) phases of the cell membrane that are enriched in sphingolipids and cholesterol (Chl), and surrounded by a liquid-disordered (L-d) phase enriched in glycerophospholipids. Lipid rafts are involved in the generation of pathological forms of proteins that are associated with neurodegenerative diseases. To investigate the effects of lipid composition and phase on the generation of pathological forms of proteins, we constructed an L-d-gel phase-separated 1-palmitoyl-2-oleoyl-sn-glycero-3-phosphocholine (POPC)/sphingomyelin (from bovine brain (BSM))-supported lipid bilayer (SLB) and an L-d-L-o phase-separated POPC/BSM/Chl SLB. We used in situ time-lapse atomic force microscopy to study the interactions between these SLBs and the prion peptide K(106)TNMKHMAGAAAAGAVVGGLG(126) (PrP106-126) amide, numbered according to the human prion-peptide sequence. Our results show that: 1), with the presence of BSM in the L-d phase, the PrP106-126 amide induces fully penetrated porations in the L-d phase of POPC/BSM SLB and POPC/BSM/Chl SLB; 2), with the presence of both BSM and Chl in the L-d phase, the PrP106-126 amide induces the disintegration of the L-d phase of POPC/BSM/Chl SLB; and 3), with the presence of both BSM and Chl in the L-o phase, PrP106-126 amide induces membrane thinning in the L-o phase of POPC/BSM/Chl SLB. These results provide comprehensive insight into the process by which the PrP106-126 amide interacts with lipid membranes.

语种英语
WOS记录号WOS:000266650400025
引用统计
被引频次:19[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
版本出版稿
条目标识符http://ir.bjmu.edu.cn/handle/400002259/50740
专题北京大学基础医学院_生物物理学系
北京大学基础医学院
作者单位1.Peking Univ, Biomed X Ctr, Sch Basic Med Sci, Dept Biophys,Single Mol & Nanobiol Lab, Beijing 100083, Peoples R China
2.Peking Univ, Ctr Prot Sci, Beijing 100083, Peoples R China
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GB/T 7714
Zhong, Jian,Yang, Chunhui,Zheng, Wenfu,et al. Effects of Lipid Composition and Phase on the Membrane Interaction of the Prion Peptide 106-126 Amide[J]. BIOPHYSICAL JOURNAL,2009,96(11):4610-4621.
APA Zhong, Jian.,Yang, Chunhui.,Zheng, Wenfu.,Huang, Lixin.,Hong, Yuankai.,...&Sha, Yinlin.(2009).Effects of Lipid Composition and Phase on the Membrane Interaction of the Prion Peptide 106-126 Amide.BIOPHYSICAL JOURNAL,96(11),4610-4621.
MLA Zhong, Jian,et al."Effects of Lipid Composition and Phase on the Membrane Interaction of the Prion Peptide 106-126 Amide".BIOPHYSICAL JOURNAL 96.11(2009):4610-4621.
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