北京大学医学部机构知识库
Advanced  
IR@PKUHSC  > 基础医学院  > 生物物理学系  > 期刊论文
学科主题: 基础医学
题名:
Effects of Lipid Composition and Phase on the Membrane Interaction of the Prion Peptide 106-126 Amide
作者: Zhong, Jian1; Yang, Chunhui1; Zheng, Wenfu1; Huang, Lixin1; Hong, Yuankai1; Wang, Lijun1; Sha, Yinlin1
刊名: BIOPHYSICAL JOURNAL
发表日期: 2009-06-03
DOI: 10.1016/j.bpj.2009.01.036
卷: 96, 期:11, 页:4610-4621
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Biophysics
研究领域[WOS]: Biophysics
关键词[WOS]: ATOMIC-FORCE MICROSCOPY ; MODEL MEMBRANES ; TRITON X-100 ; ANTIMICROBIAL PEPTIDES ; ALZHEIMERS-DISEASE ; SUPPORTED BILAYERS ; PROTEIN-FRAGMENT ; AMYLOID PEPTIDE ; CELL-MEMBRANES ; RAFT FORMATION
英文摘要:

Lipid rafts are specialized liquid-ordered (L-o) phases of the cell membrane that are enriched in sphingolipids and cholesterol (Chl), and surrounded by a liquid-disordered (L-d) phase enriched in glycerophospholipids. Lipid rafts are involved in the generation of pathological forms of proteins that are associated with neurodegenerative diseases. To investigate the effects of lipid composition and phase on the generation of pathological forms of proteins, we constructed an L-d-gel phase-separated 1-palmitoyl-2-oleoyl-sn-glycero-3-phosphocholine (POPC)/sphingomyelin (from bovine brain (BSM))-supported lipid bilayer (SLB) and an L-d-L-o phase-separated POPC/BSM/Chl SLB. We used in situ time-lapse atomic force microscopy to study the interactions between these SLBs and the prion peptide K(106)TNMKHMAGAAAAGAVVGGLG(126) (PrP106-126) amide, numbered according to the human prion-peptide sequence. Our results show that: 1), with the presence of BSM in the L-d phase, the PrP106-126 amide induces fully penetrated porations in the L-d phase of POPC/BSM SLB and POPC/BSM/Chl SLB; 2), with the presence of both BSM and Chl in the L-d phase, the PrP106-126 amide induces the disintegration of the L-d phase of POPC/BSM/Chl SLB; and 3), with the presence of both BSM and Chl in the L-o phase, PrP106-126 amide induces membrane thinning in the L-o phase of POPC/BSM/Chl SLB. These results provide comprehensive insight into the process by which the PrP106-126 amide interacts with lipid membranes.

语种: 英语
所属项目编号: 20273002 ; 20103001
项目资助者: Natural Science Foundation of China
WOS记录号: WOS:000266650400025
Citation statistics:
内容类型: 期刊论文
版本: 出版稿
URI标识: http://ir.bjmu.edu.cn/handle/400002259/50740
Appears in Collections:基础医学院_生物物理学系_期刊论文

Files in This Item:
File Name/ File Size Content Type Version Access License
Effects of Lipid Composition and Phase on the Membrane Interaction of the .pdf(1321KB)期刊论文出版稿限制开放 联系获取全文

作者单位: 1.Peking Univ, Biomed X Ctr, Sch Basic Med Sci, Dept Biophys,Single Mol & Nanobiol Lab, Beijing 100083, Peoples R China
2.Peking Univ, Ctr Prot Sci, Beijing 100083, Peoples R China

Recommended Citation:
Zhong, Jian,Yang, Chunhui,Zheng, Wenfu,et al. Effects of Lipid Composition and Phase on the Membrane Interaction of the Prion Peptide 106-126 Amide[J]. BIOPHYSICAL JOURNAL,2009,96(11):4610-4621.
Service
Recommend this item
Sava as my favorate item
Show this item's statistics
Export Endnote File
Google Scholar
Similar articles in Google Scholar
[Zhong, Jian]'s Articles
[Yang, Chunhui]'s Articles
[Zheng, Wenfu]'s Articles
CSDL cross search
Similar articles in CSDL Cross Search
[Zhong, Jian]‘s Articles
[Yang, Chunhui]‘s Articles
[Zheng, Wenfu]‘s Articles
Related Copyright Policies
Null
Social Bookmarking
Add to CiteULike Add to Connotea Add to Del.icio.us Add to Digg Add to Reddit

Items in IR are protected by copyright, with all rights reserved, unless otherwise indicated.

 

 

Valid XHTML 1.0!
Copyright © 2007-2017  北京大学医学部 - Feedback
Powered by CSpace