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学科主题: 基础医学
题名:
IgG gene expression and its possible significance in prostate cancers
作者: Liu, Yuxuan1; Chen, Zhengshan2; Niu, Na1; Chang, Qing1; Deng, Ruishu1; Korteweg, Christine2; Gu, Jiang1,2
关键词: immunoglobulin G ; prostate cancer ; biomarker ; Gleason score ; anti-IgG antibody
刊名: PROSTATE
发表日期: 2012-05-01
DOI: 10.1002/pros.21476
卷: 72, 期:6, 页:690-701
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Endocrinology & Metabolism ; Urology & Nephrology
研究领域[WOS]: Endocrinology & Metabolism ; Urology & Nephrology
关键词[WOS]: LINKED OLIGOSACCHARIDE CHAINS ; IMMUNOGLOBULIN BINDING-FACTOR ; HUMAN SEMINAL PLASMA ; ALPHA HEAVY-CHAIN ; ALPHA-2-MACROGLOBULIN DEFICIENCY ; CELL-LINES ; SERA ; CARCINOGENESIS ; ADENOCARCINOMA ; IDENTIFICATION
英文摘要:

BACKGROUND In spite of recent advances in treatment strategies, prostate cancer (PCa) remains the second leading cause of cancer death in men with its genetic and biologic behaviors still poorly understood. Recently, accumulating evidence indicates that cancer cells, as well as some normal cells can secret IgG. This study was designed to evaluate IgG gene expression and its possible significance in PCa tissue samples and cell lines.

METHODS. IgG expression was assessed by immunohistochemistry, in situ hybridization, immunofluorescence, RT-PCR, and Western blot. The possible significance of IgG was evaluated on tissue array and cell lines. To assess cell viability and proliferation, MTS assay was carried out. Apoptosis was evaluated with propidium iodide and annexin-V staining.

RESULTS. Expressions of IgG and its related genes were detected in cell lines. Abundant gene expressions of Igg and Igk chain were detected in PCa tissue samples, but not in normal prostate tissues. In addition, IgG expression was significantly higher in PCa tissues than in the benign prostate hyperplasia tissues (P < 0.001). Igg expression was positively correlated to Gleason score and histological grade (P < 0.05). Furthermore, in vitro experiments showed that anti-human monoclonal IgG antibody suppressed cell proliferation and increased apoptosis in cultured PCa cells.

CONCLUSION. IgG gene expression in PCa is related to cell differentiation and clinical status. PCa cell produced IgG is involved in the biological behavior of this cancer and may serve as a useful marker for cancer cell differentiation and prognosis. Locally produced IgG could be a potential target for therapy. Prostate 72:690-701, 2012. (C) 2011 Wiley Periodicals, Inc.

语种: 英语
所属项目编号: B 07001 ; 30971150 ; 81001199 ; 30950110335
项目资助者: International Collaboration Project (111 Project) ; National Natural Science Foundation of China
WOS记录号: WOS:000301712500012
Citation statistics:
内容类型: 期刊论文
版本: 出版稿
URI标识: http://ir.bjmu.edu.cn/handle/400002259/50758
Appears in Collections:基础医学院_病理学系_期刊论文

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作者单位: 1.Shantou Univ Med Coll, Dept Pathol, Shantou, Peoples R China
2.Peking Univ, Dept Pathol, Sch Basic Med Sci, Beijing 100871, Peoples R China

Recommended Citation:
Liu, Yuxuan,Chen, Zhengshan,Niu, Na,et al. IgG gene expression and its possible significance in prostate cancers[J]. PROSTATE,2012,72(6):690-701.
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