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学科主题: 药学
题名:
Lanthanide ions induce hydrolysis of hemoglobin-bound 2,3-diphosphoglycerate (2,3-DPG), conformational changes of globin and bidirectional changes of 2,3-DPG-hemoglobin′s oxygen affinity
作者: Cheng, Y1; Lin, HK1; Xue, DP1; Li, RC1; Wang, K1
关键词: lanthanide ion ; hemoglobin ; conformational change ; 2,3-diphosphoglycerate hydrolysis ; oxygen affinity
刊名: BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR BASIS OF DISEASE
发表日期: 2001-02-14
卷: 1535, 期:2, 页:200-216
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Biochemistry & Molecular Biology ; Biophysics ; Cell Biology
研究领域[WOS]: Biochemistry & Molecular Biology ; Biophysics ; Cell Biology
关键词[WOS]: MAGNETIC-RESONANCE SPECTROSCOPY ; HUMAN ERYTHROCYTE-MEMBRANE ; ALLOSTERIC MECHANISM ; ORGANIC-PHOSPHATES ; BINDING ; DEOXYHEMOGLOBIN ; COMPLEXES ; TRANSPORT ; MYOGLOBIN ; SPECTRA
英文摘要:

The changes in structure and function of 2,3-diphosphoglycerate-hemoglobin (2,3-DPG-Hb) induced by Ln(3+) binding were studied by spectroscopic methods. The binding of lanthanide cations to 2,3-DPG is prior to that to Hb. Ln(3+) binding causes the hydrolysis of either one from the two phosphomonoester bonds in 2,3-DPG non-specifically. The results using the ultrafiltration method indicate that Ln(3+) binding sites for Hb can be classified into three categories: i.e. positive cooperative sites (N-I), non-cooperative strong sites (N-S) and non-cooperative weak sites (N-W) with binding constants in decreasing order: K-I > K-S > K-W. The total number of binding sites amounts to about 65 per Hb tetramer. Information on reaction kinetics was obtained from the change of intrinsic fluorescence in Hb monitored by stopped-flow fluorometry. Fluctuation of fluorescence dependent on Ln(3+) concentration and temperature was observed and can be attributed to the successive conformational changes induced by Ln(3+) binding. The results also reveal the bidirectional changes of the oxygen affinity of Hb in the dependence on Ln(3+) concentration. At the range of [Ln(3+)]/[Hb] < 2, the marked increase of oxygen affinity (P-50 decrease) with the Ln(3+) concentration can be attributed to the hydrolysis of 2,3-DPG, while the slight rebound of oxygen affinity in higher Ln(3+) concentration can be interpreted by the transition to the T-state of the Hb tetramer induced by Ln(3+) binding. This was indicated by the changes in secondary structure characterized by the decrease of -helix content. (C) 2001 Elsevier Science B.V. All rights reserved.

语种: 英语
WOS记录号: WOS:000167031700013
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/50768
Appears in Collections:北京大学药学院_期刊论文

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作者单位: 1.Nankai Univ, Dept Chem, Tianjin 300071, Peoples R China
2.Peking Univ, Sch Pharmaceut Sci, Natl Res Labs Nat & Biomimet Drugs, Beijing 100083, Peoples R China

Recommended Citation:
Cheng, Y,Lin, HK,Xue, DP,et al. Lanthanide ions induce hydrolysis of hemoglobin-bound 2,3-diphosphoglycerate (2,3-DPG), conformational changes of globin and bidirectional changes of 2,3-DPG-hemoglobin&prime;s oxygen affinity[J]. BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR BASIS OF DISEASE,2001,1535(2):200-216.
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