IR@PKUHSC  > 北京大学药学院
学科主题药学
Lanthanide ions induce hydrolysis of hemoglobin-bound 2,3-diphosphoglycerate (2,3-DPG), conformational changes of globin and bidirectional changes of 2,3-DPG-hemoglobin′s oxygen affinity
Cheng, Y1; Lin, HK1; Xue, DP1; Li, RC1; Wang, K1
关键词Lanthanide Ion Hemoglobin Conformational Change Oxygen Affinity 2 3-diphosphoglycerate Hydrolysis
刊名BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR BASIS OF DISEASE
2001-02-14
1535期:2页:200-216
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Biochemistry & Molecular Biology ; Biophysics ; Cell Biology
研究领域[WOS]Biochemistry & Molecular Biology ; Biophysics ; Cell Biology
关键词[WOS]MAGNETIC-RESONANCE SPECTROSCOPY ; HUMAN ERYTHROCYTE-MEMBRANE ; ALLOSTERIC MECHANISM ; ORGANIC-PHOSPHATES ; BINDING ; DEOXYHEMOGLOBIN ; COMPLEXES ; TRANSPORT ; MYOGLOBIN ; SPECTRA
英文摘要

The changes in structure and function of 2,3-diphosphoglycerate-hemoglobin (2,3-DPG-Hb) induced by Ln(3+) binding were studied by spectroscopic methods. The binding of lanthanide cations to 2,3-DPG is prior to that to Hb. Ln(3+) binding causes the hydrolysis of either one from the two phosphomonoester bonds in 2,3-DPG non-specifically. The results using the ultrafiltration method indicate that Ln(3+) binding sites for Hb can be classified into three categories: i.e. positive cooperative sites (N-I), non-cooperative strong sites (N-S) and non-cooperative weak sites (N-W) with binding constants in decreasing order: K-I > K-S > K-W. The total number of binding sites amounts to about 65 per Hb tetramer. Information on reaction kinetics was obtained from the change of intrinsic fluorescence in Hb monitored by stopped-flow fluorometry. Fluctuation of fluorescence dependent on Ln(3+) concentration and temperature was observed and can be attributed to the successive conformational changes induced by Ln(3+) binding. The results also reveal the bidirectional changes of the oxygen affinity of Hb in the dependence on Ln(3+) concentration. At the range of [Ln(3+)]/[Hb] < 2, the marked increase of oxygen affinity (P-50 decrease) with the Ln(3+) concentration can be attributed to the hydrolysis of 2,3-DPG, while the slight rebound of oxygen affinity in higher Ln(3+) concentration can be interpreted by the transition to the T-state of the Hb tetramer induced by Ln(3+) binding. This was indicated by the changes in secondary structure characterized by the decrease of -helix content. (C) 2001 Elsevier Science B.V. All rights reserved.

语种英语
WOS记录号WOS:000167031700013
引用统计
被引频次:23[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/50768
专题北京大学药学院
作者单位1.Nankai Univ, Dept Chem, Tianjin 300071, Peoples R China
2.Peking Univ, Sch Pharmaceut Sci, Natl Res Labs Nat & Biomimet Drugs, Beijing 100083, Peoples R China
推荐引用方式
GB/T 7714
Cheng, Y,Lin, HK,Xue, DP,et al. Lanthanide ions induce hydrolysis of hemoglobin-bound 2,3-diphosphoglycerate (2,3-DPG), conformational changes of globin and bidirectional changes of 2,3-DPG-hemoglobin&prime;s oxygen affinity[J]. BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR BASIS OF DISEASE,2001,1535(2):200-216.
APA Cheng, Y,Lin, HK,Xue, DP,Li, RC,&Wang, K.(2001).Lanthanide ions induce hydrolysis of hemoglobin-bound 2,3-diphosphoglycerate (2,3-DPG), conformational changes of globin and bidirectional changes of 2,3-DPG-hemoglobin′s oxygen affinity.BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR BASIS OF DISEASE,1535(2),200-216.
MLA Cheng, Y,et al."Lanthanide ions induce hydrolysis of hemoglobin-bound 2,3-diphosphoglycerate (2,3-DPG), conformational changes of globin and bidirectional changes of 2,3-DPG-hemoglobin′s oxygen affinity".BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR BASIS OF DISEASE 1535.2(2001):200-216.
条目包含的文件
条目无相关文件。
个性服务
推荐该条目
保存到收藏夹
查看访问统计
导出为Endnote文件
谷歌学术
谷歌学术中相似的文章
[Cheng, Y]的文章
[Lin, HK]的文章
[Xue, DP]的文章
百度学术
百度学术中相似的文章
[Cheng, Y]的文章
[Lin, HK]的文章
[Xue, DP]的文章
必应学术
必应学术中相似的文章
[Cheng, Y]的文章
[Lin, HK]的文章
[Xue, DP]的文章
相关权益政策
暂无数据
收藏/分享
所有评论 (0)
暂无评论
 

除非特别说明,本系统中所有内容都受版权保护,并保留所有权利。