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学科主题: 临床医学
题名:
Quantitative proteomic analysis of mitochondria from human ovarian cancer cells and their paclitaxel-resistant sublines
作者: Chen, Ming1,2; Huang, Hong1,2,3; He, Haojie1,2,4; Ying, Wantao5; Liu, Xin5,6; Dai, Zhiqin1,2,7,8; Yin, Jie1,2; Mao, Ning9; Qian, Xiaohong5; Pan, Lingya1,2
关键词: Drug resistance ; liquid chromatography-hybrid linear ion trap Fourier-transform ion cyclotron resonance mass spectrometry ; mitochondria proteins ; ovarian cancer ; paclitaxel
刊名: CANCER SCIENCE
发表日期: 2015-08-01
DOI: 10.1111/cas.12710
卷: 106, 期:8, 页:1075-1083
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Oncology
研究领域[WOS]: Oncology
关键词[WOS]: 14-3-3 ZETA PROTEIN ; EPITHELIAL OVARIAN ; CHEMOTHERAPY RESPONSE ; TAXOL RESISTANCE ; DRUG-RESISTANCE ; CARCINOMA ; PLATINUM ; TUMOR ; PROLIFERATION ; MECHANISMS
英文摘要:

Paclitaxel resistance is a major obstacle for the treatment of ovarian cancer. The chemoresistance mechanisms are partly related to the mitochondria. Identification of the relevant proteins in mitochondria will help in clarifying the possible mechanisms and in selecting effective chemotherapy for patients with paclitaxel resistance. In the present study, mitochondria from two paclitaxel-sensitive human ovarian cancer cell lines (SKOV3 and A2780) and their corresponding resistant cell lines (SKOV3-TR and A2780-TR) were isolated. Guanidine-modified acetyl-stable isotope labeling and liquid chromatography-hybrid linear ion trap Fourier-transform ion cyclotron resonance mass spectrometry (LC-FTICR MS) were performed to find the expressed differential proteins. Comparative proteomic analysis revealed eight differentially expressed proteins in the ovarian cancer cells and their paclitaxel-resistant sublines. Among them, mimitin and 14-3-3 / were selected for further research. The effects of mimitin and 14-3-3 / were explored using specific siRNA interference in ovarian cancer cell lines and immunohistochemistry in human tissue specimens. The downregulation of mimitin and 14-3-3 / using specific siRNA in paclitaxel-resistant ovarian cancer cells led to an increase in the resistance index to paclitaxel. Multivariate analyses demonstrated that lower expression levels of the mimitin and 14-3-3 / proteins were positively associated with shorter progression-free survival (PFS) and overall survival (OS) in patients with primary ovarian cancer (mimitin: PFS: P=0.041, OS: P=0.003; 14-3-3 /: PFS: P=0.031, OS: P=0.011). Mimitin and 14-3-3 protein / are potential markers of paclitaxel resistance and prognostic factors in ovarian cancer.

语种: 英语
所属项目编号: 30571944
项目资助者: National Natural Science Foundation of China
WOS记录号: WOS:000359867000017
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/50791
Appears in Collections:北京大学第三临床医学院_期刊论文

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作者单位: 1.Yantai Yuhuangding Hosp, Cent Lab, Yantai, Peoples R China
2.Jiangsu Canc Hosp, Dept Gynecol Oncol, Nanjing, Jiangsu, Peoples R China
3.Jiangsu Inst Canc Res, Nanjing, Jiangsu, Peoples R China
4.Chinese Acad Med Sci, Dept Obstet & Gynecol, Peking Union Med Coll Hosp, Beijing 100730, Peoples R China
5.Peking Union Med Coll, Beijing 100730, Peoples R China
6.Capital Med Univ, Beijing Obstet & Gynecol Hosp, Dept Gynecol, Beijing, Peoples R China
7.Peking Univ, Hosp 3, Dept Obstet & Gynecol, Beijing 100871, Peoples R China
8.Beijing Proteome Res Ctr, State Key Lab Proteom, Beijing, Peoples R China
9.Chinese Acad Med Sci, Inst Basic Med Sci, Dept Cell Biol, Beijing 100730, Peoples R China

Recommended Citation:
Chen, Ming,Huang, Hong,He, Haojie,et al. Quantitative proteomic analysis of mitochondria from human ovarian cancer cells and their paclitaxel-resistant sublines[J]. CANCER SCIENCE,2015,106(8):1075-1083.
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