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学科主题: 基础医学
题名:
The Largely Normal Response to Toll-Like Receptor 7 and 9 Stimulation and the Enhanced Expression of SIGIRR by B Cells in Systemic Lupus Erythematosus
作者: Zhu, Yun-Yan1,2; Su, Yin3; Li, Zhan-Guo3; Zhang, Yu1,2
刊名: PLOS ONE
发表日期: 2012-08-29
DOI: 10.1371/journal.pone.0044131
卷: 7, 期:8
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Multidisciplinary Sciences
研究领域[WOS]: Science & Technology - Other Topics
关键词[WOS]: BLOOD MONONUCLEAR-CELLS ; PERIPHERAL-BLOOD ; MURINE LUPUS ; IMMUNE-RESPONSES ; EPITHELIAL-CELLS ; FAMILY-MEMBER ; UP-REGULATION ; INFLAMMATION ; TIR8/SIGIRR ; TLR7
英文摘要:

Background: Altered Toll-like receptor (TLR) signaling has been implicated in the pathogenesis of systemic lupus erythematosus (SLE). The present study was undertaken to characterize responses of B cells from SLE patients to TLR7 and TLR9 stimulation and to explore the potential role of single immunoglobulin interleukin-1 receptor related molecule (SIGIRR) in the regulation of TLR-mediated responses of SLE B cells.

Methodology/Principal Findings: Peripheral blood mononuclear cells (PBMC) were isolated from 64 patients with SLE and 37 healthy donors. CD19+ B cells purified using microbeads were cultured with TLR7 or TLR9 agonists. Cell proliferation was measured by thymine incorporation and the frequency of antibody-secreting cells was determined by ELISPOT assay. SIGIRR expression in PBMCs and B cells was analyzed using flow cytometry analysis. In contrast to the enhanced proliferation following B cell receptor (BCR) engagement, B cells from SLE patients exhibited a virtually normal proliferative response to TLR7 or TLR9 stimulation. Moreover, B cells from SLE patients and healthy donors were almost equally competent to differentiate into antibody-secreting cells upon TLR engagement except for a reduction in the generation of IgG-secreting cells by TLR9-stimulated lupus B cells. In line with these somehow unexpected observations, SLE B cells were found to express a significantly higher level of SIGIRR than normal B cells.

Conclusions/Significance: Despite the reported upregulation of TLR7 and TLR9 expression in B cell from SLE patients, their responses to TLR stimulation were largely normal. The increased expression of the negative regulator SIGIRR may be partly responsible for the "balance of terror".

语种: 英语
所属项目编号: 30830091 ; 81172844 ; 2011CB946100
项目资助者: National Science Foundation of China ; Ministry of Science and Technology of China
WOS记录号: WOS:000308206000088
Citation statistics:
内容类型: 期刊论文
版本: 出版稿
URI标识: http://ir.bjmu.edu.cn/handle/400002259/50793
Appears in Collections:基础医学院_免疫学系_期刊论文

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作者单位: 1.Peking Univ, Hlth Sci Ctr, Dept Immunol, Minist Hlth, Beijing 100871, Peoples R China
2.Peking Univ, Hlth Sci Ctr, Minist Hlth, Key Lab Med Immunol, Beijing 100871, Peoples R China
3.Peking Univ, Peoples Hosp, Dept Rheumatol & Immunol, Beijing 100871, Peoples R China

Recommended Citation:
Zhu, Yun-Yan,Su, Yin,Li, Zhan-Guo,et al. The Largely Normal Response to Toll-Like Receptor 7 and 9 Stimulation and the Enhanced Expression of SIGIRR by B Cells in Systemic Lupus Erythematosus[J]. PLOS ONE,2012,7(8).
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