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Polyaspartoyl.L-arginine enhances prostacyclin synthesis in rat aortic endothelial cells
Tang, Zhiyu1; Yang, Jian1; Liu, Xiaoyan1; Wang, Yinye1; Peng, Shiqi2
关键词Polyaspartoyl.l-arginine Nitric Oxide Prostacyclin Rat Aortic Endothelial Cells
刊名EUROPEAN JOURNAL OF PHARMACOLOGY
2008-12-28
DOI10.1016/j.ejphar.2008.11.003
601期:1-3页:124-128
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Pharmacology & Pharmacy
研究领域[WOS]Pharmacology & Pharmacy
关键词[WOS]NITRIC-OXIDE SYNTHASE ; PLATELET-AGGREGATION ; RELEASE ; CYCLOOXYGENASE ; INHIBITION ; ACTIVATION ; MECHANISM ; CULTURE ; ASSAY
英文摘要

Nitricoxide (NO) and prostacyclin(PGl(2)) are two of the most important vasodilators produced by endothelial cells, the regulation of NO on PGl(2) production has not been fully clear yet Polyaspartoyl.L-arginine (PDR) is an L-arginine residue-rich compound with inhibitory effects of platelet aggregation and thrombosis. This study investigated its effects on NO production in rat aortic endothelial cells (RAECs) and observed the influence of NO on PGI(2) level in RAECs. NO concentration in the medium of RAECs was assessed with fluorometric method; 6-keto-PGF(1 alpha), the stable metabolite of PGl(2), in the medium of RAECs was measured with radioimmunoassay kits; Protein level of PGl(2) synthase in RAECs was determined by Western blot analysis. PDR (17.0 similar to 170 mu g/ml, equal to 0.5 mu M-5 PM) enhanced NO level in culture medium of RAEC with concentration-dependent manner (P<0.01); L-arginine (170 mu g/ml, equal to 1000 mu M) and 1.70 mu g/ml (0.05 mu M) of PDR slightly increased NO level (P>0.05). Interestingly PDR (1.70-500 mu g/ml), L-arginine (17.0-170 mu g/ml) significantly elevated PGl(2) levels in medium of RAECs (P<0.01), NO synthase inhibitor, N-G-nitro L-arginine methyl ester (L-NAME), markedly inhibited the elevated PGl(2) levels by PDR and L-arginine. NO donor. sodium nitroprusside(SNP)(1-500 mu M), showed the most powerful effects of increasing PGl(2) level in RAECs, which was not influenced by L-NAME. Cyclooxigenase(COX) inhibitor, indomethacin, significantly reduced elevated PGl(2) level by both PDR and SNP in RAEC medium. PDR (170 mu g/ml) increased the expression of PGl(2) synthase, L-NAME partly inhibited this effect. In conclusion, PDR enhances PGl(2) synthesis in RAEC, which is attributed to its effect of NO production; the stimulating effect of PDR on PGl(2) synthesis may be mediated via COX and PGl(2) synthase. (C) 2008 Published by Elsevier B.V.

语种英语
WOS记录号WOS:000261825000020
引用统计
被引频次:3[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/50800
专题北京大学药学院
北京大学药学院_分子与细胞药理学系
医学人文研究院/公共教学部_哲学与社会科学系
作者单位1.Peking Univ, Sch Pharmaceut Sci, Dept Mol & Cellular Pharmacol, Beijing 100191, Peoples R China
2.Capital Univ Med Sci, Beijing Key Labs Hydrone & Peptides, Beijing 100069, Peoples R China
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GB/T 7714
Tang, Zhiyu,Yang, Jian,Liu, Xiaoyan,et al. Polyaspartoyl.L-arginine enhances prostacyclin synthesis in rat aortic endothelial cells[J]. EUROPEAN JOURNAL OF PHARMACOLOGY,2008,601(1-3):124-128.
APA Tang, Zhiyu,Yang, Jian,Liu, Xiaoyan,Wang, Yinye,&Peng, Shiqi.(2008).Polyaspartoyl.L-arginine enhances prostacyclin synthesis in rat aortic endothelial cells.EUROPEAN JOURNAL OF PHARMACOLOGY,601(1-3),124-128.
MLA Tang, Zhiyu,et al."Polyaspartoyl.L-arginine enhances prostacyclin synthesis in rat aortic endothelial cells".EUROPEAN JOURNAL OF PHARMACOLOGY 601.1-3(2008):124-128.
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