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学科主题: 药学
题名:
Polyaspartoyl.L-arginine enhances prostacyclin synthesis in rat aortic endothelial cells
作者: Tang, Zhiyu1; Yang, Jian1; Liu, Xiaoyan1; Wang, Yinye1; Peng, Shiqi2
关键词: Polyaspartoyl.L-arginine ; Nitric oxide ; Prostacyclin ; Rat aortic endothelial cells
刊名: EUROPEAN JOURNAL OF PHARMACOLOGY
发表日期: 2008-12-28
DOI: 10.1016/j.ejphar.2008.11.003
卷: 601, 期:1-3, 页:124-128
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Pharmacology & Pharmacy
研究领域[WOS]: Pharmacology & Pharmacy
关键词[WOS]: NITRIC-OXIDE SYNTHASE ; PLATELET-AGGREGATION ; RELEASE ; CYCLOOXYGENASE ; INHIBITION ; ACTIVATION ; MECHANISM ; CULTURE ; ASSAY
英文摘要:

Nitricoxide (NO) and prostacyclin(PGl(2)) are two of the most important vasodilators produced by endothelial cells, the regulation of NO on PGl(2) production has not been fully clear yet Polyaspartoyl.L-arginine (PDR) is an L-arginine residue-rich compound with inhibitory effects of platelet aggregation and thrombosis. This study investigated its effects on NO production in rat aortic endothelial cells (RAECs) and observed the influence of NO on PGI(2) level in RAECs. NO concentration in the medium of RAECs was assessed with fluorometric method; 6-keto-PGF(1 alpha), the stable metabolite of PGl(2), in the medium of RAECs was measured with radioimmunoassay kits; Protein level of PGl(2) synthase in RAECs was determined by Western blot analysis. PDR (17.0 similar to 170 mu g/ml, equal to 0.5 mu M-5 PM) enhanced NO level in culture medium of RAEC with concentration-dependent manner (P<0.01); L-arginine (170 mu g/ml, equal to 1000 mu M) and 1.70 mu g/ml (0.05 mu M) of PDR slightly increased NO level (P>0.05). Interestingly PDR (1.70-500 mu g/ml), L-arginine (17.0-170 mu g/ml) significantly elevated PGl(2) levels in medium of RAECs (P<0.01), NO synthase inhibitor, N-G-nitro L-arginine methyl ester (L-NAME), markedly inhibited the elevated PGl(2) levels by PDR and L-arginine. NO donor. sodium nitroprusside(SNP)(1-500 mu M), showed the most powerful effects of increasing PGl(2) level in RAECs, which was not influenced by L-NAME. Cyclooxigenase(COX) inhibitor, indomethacin, significantly reduced elevated PGl(2) level by both PDR and SNP in RAEC medium. PDR (170 mu g/ml) increased the expression of PGl(2) synthase, L-NAME partly inhibited this effect. In conclusion, PDR enhances PGl(2) synthesis in RAEC, which is attributed to its effect of NO production; the stimulating effect of PDR on PGl(2) synthesis may be mediated via COX and PGl(2) synthase. (C) 2008 Published by Elsevier B.V.

语种: 英语
WOS记录号: WOS:000261825000020
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/50800
Appears in Collections:北京大学药学院_期刊论文

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作者单位: 1.Peking Univ, Sch Pharmaceut Sci, Dept Mol & Cellular Pharmacol, Beijing 100191, Peoples R China
2.Capital Univ Med Sci, Beijing Key Labs Hydrone & Peptides, Beijing 100069, Peoples R China

Recommended Citation:
Tang, Zhiyu,Yang, Jian,Liu, Xiaoyan,et al. Polyaspartoyl.L-arginine enhances prostacyclin synthesis in rat aortic endothelial cells[J]. EUROPEAN JOURNAL OF PHARMACOLOGY,2008,601(1-3):124-128.
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