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学科主题临床医学
Characterization of Hydrogen Sulfide and Its Synthases, Cystathionine beta-Synthase and Cystathionine gamma-Lyase, in Human Prostatic Tissue and Cells
Guo, Hui1; Gai, Jun-Wei1; Wang, Ying1; Jin, Hong-Fang1; Du, Jun-Bao1; Jin, Jie1
刊名UROLOGY
2012-02-01
DOI10.1016/j.urology.2011.10.013
79期:2
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Urology & Nephrology
资助者National Natural Science Foundation of China ; National Natural Science Foundation of China
研究领域[WOS]Urology & Nephrology
关键词[WOS]TESTOSTERONE REGULATION ; H2S ; APOPTOSIS ; VASORELAXANT
英文摘要

OBJECTIVE To investigate hydrogen sulfide and its synthases, cystathionine beta-synthase (CBS) and cystathionine gamma-lyase (CSE), in human prostatic tissue and cells.

METHODS CBS and CSE in human prostatic tissue and cells were located using immunostaining. Western blot and a sulfur-sensitive electrode were used to evaluate the expression levels and catalytic activity of CBS and CSE. We analyzed the association between dihydrotestoster-one-added or hormone-reduced medium-induced CBS/CSE protein levels with androgen receptor levels in prostate cancer lines. All experiments were repeated >= 3 times.

RESULTS Endogenous hydrogen sulfide and its synthases existed in various areas of human prostatic tissue and cells. Cell activity and CBS/CSE protein levels were greatest in the androgen-dependent prostate cancer cell LNCaP among all cells and downregulated by dihydrotestosterone.

CONCLUSION Hydrogen sulfide and its synthases in human prostatic tissue and cells were modulated by dihydrotestosterone, which could suggest a potential therapy for prostatic disease. UROLOGY 79: 483.e1-483.e5, 2012. (c) 2012 Elsevier Inc.

语种英语
所属项目编号30571851
资助者National Natural Science Foundation of China ; National Natural Science Foundation of China
WOS记录号WOS:000300407200077
引用统计
被引频次:14[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/50805
专题北京大学第一临床医学院_泌尿外科
作者单位1.Peking Univ, Inst Urol, Beijing 100034, Peoples R China
2.Peking Univ, Inst Urol, Hosp 1, Dept Urol, Beijing 100034, Peoples R China
3.Peking Univ, Hosp 1, Dept Pediat, Beijing 100034, Peoples R China
推荐引用方式
GB/T 7714
Guo, Hui,Gai, Jun-Wei,Wang, Ying,et al. Characterization of Hydrogen Sulfide and Its Synthases, Cystathionine beta-Synthase and Cystathionine gamma-Lyase, in Human Prostatic Tissue and Cells[J]. UROLOGY,2012,79(2).
APA Guo, Hui,Gai, Jun-Wei,Wang, Ying,Jin, Hong-Fang,Du, Jun-Bao,&Jin, Jie.(2012).Characterization of Hydrogen Sulfide and Its Synthases, Cystathionine beta-Synthase and Cystathionine gamma-Lyase, in Human Prostatic Tissue and Cells.UROLOGY,79(2).
MLA Guo, Hui,et al."Characterization of Hydrogen Sulfide and Its Synthases, Cystathionine beta-Synthase and Cystathionine gamma-Lyase, in Human Prostatic Tissue and Cells".UROLOGY 79.2(2012).
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