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Spray-Dried Chitosan Microparticles for Cellular Delivery of an Antigenic Protein: Physico-chemical Properties and Cellular Uptake by Dendritic Cells and Macrophages
Kusonwiriyawong, Chirasak1; Lipipun, Vimolmas2; Vardhanabhuti, Nontima1; Zhang, Qiang3; Ritthidej, Garnpimol C.1
关键词Cellular Uptake Chitosan Microparticles Dendritic Cell Macrophage Spray Dry
刊名PHARMACEUTICAL RESEARCH
2013-06-01
DOI10.1007/s11095-013-1014-7
30期:6页:1677-1697
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Chemistry, Multidisciplinary ; Pharmacology & Pharmacy
研究领域[WOS]Chemistry ; Pharmacology & Pharmacy
关键词[WOS]RECOMBINANT HUMAN GELATINS ; DRUG-DELIVERY ; POLYMERIC NANOPARTICLES ; ISOELECTRIC POINTS ; PLGA MICROSPHERES ; IMMUNE-RESPONSES ; ORAL DELIVERY ; VACCINE ; STABILIZATION ; STABILITY
英文摘要

Spray-dried chitosan microparticles for cellular delivery of antigen to dendritic cells (DC) and macrophages (MI center dot) were investigated.

Chitosan microparticles were prepared by spray drying. For comparison, poly(lactic-co-glycolic acid) (PLGA) and poly(alpha-butyl cyanoacrylate) (BCA) micro-/nanoparticles were generated. Bovine serum albumin (BSA) was used as a model antigen. The particles were characterized in terms of size, morphology, surface charge, surface composition, protein content, entrapment efficiency, in vitro release, and protein integrity. Additionally, they were subject to cell viability and cellular uptake study with DC and MI center dot.

Size of chitosan, PLGA, and BCA micro-/nanoparticles ranged between 3.11-7.18, 0.94-6.26, and 0.30-6.34 mu m, respectively. Particle morphology and in vitro protein release varied, depending on polymer type, particle composition and preparation process parameters. Chitosan microparticles were cationic, while PLGA microparticles were neutral. BCA micro-/nanoparticles were either anionic or cationic, according to polymerization pH. Protein content and entrapment efficiency of chitosan and PLGA microparticles were relatively consistent. Only integrity and conformational structure of protein encapsulated in chitosan microparticles were completely retained. Chitosan and PLGA microparticles were non-toxic to DC and MI center dot, but the former were internalized more efficiently.

Spray-dried chitosan microparticles delivered the antigen efficiently to DC and M phi.

语种英语
WOS记录号WOS:000318795400019
项目编号HR 1166I
资助机构Thailand Research Funds (TRF) through the Royal Golden Jubilee PhD Program, Office of the Higher Education Commission through the National Research University Project ; Ratchadaphiseksomphot Endowment Fund ; Chulalongkorn University
引用统计
被引频次:9[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/50833
专题北京大学药学院_药剂学系
作者单位1.Chulalongkorn Univ, Fac Pharmaceut Sci, Dept Pharmaceut & Ind Pharm, Bangkok 10330, Thailand
2.Chulalongkorn Univ, Fac Pharmaceut Sci, Dept Biochem & Microbiol, Bangkok 10330, Thailand
3.Peking Univ, Dept Pharmaceut, Sch Pharmaceut Sci, Beijing 100083, Peoples R China
推荐引用方式
GB/T 7714
Kusonwiriyawong, Chirasak,Lipipun, Vimolmas,Vardhanabhuti, Nontima,et al. Spray-Dried Chitosan Microparticles for Cellular Delivery of an Antigenic Protein: Physico-chemical Properties and Cellular Uptake by Dendritic Cells and Macrophages[J]. PHARMACEUTICAL RESEARCH,2013,30(6):1677-1697.
APA Kusonwiriyawong, Chirasak,Lipipun, Vimolmas,Vardhanabhuti, Nontima,Zhang, Qiang,&Ritthidej, Garnpimol C..(2013).Spray-Dried Chitosan Microparticles for Cellular Delivery of an Antigenic Protein: Physico-chemical Properties and Cellular Uptake by Dendritic Cells and Macrophages.PHARMACEUTICAL RESEARCH,30(6),1677-1697.
MLA Kusonwiriyawong, Chirasak,et al."Spray-Dried Chitosan Microparticles for Cellular Delivery of an Antigenic Protein: Physico-chemical Properties and Cellular Uptake by Dendritic Cells and Macrophages".PHARMACEUTICAL RESEARCH 30.6(2013):1677-1697.
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