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学科主题: 基础医学
题名:
Plasmid-based Stat3 siRNA delivered by hydroxyapatite nanoparticles suppresses mouse prostate tumour growth in vivo
作者: Liang, Zuo-Wen1,2; Guo, Bao-Feng3; Li, Yang1,2; Li, Xiao-Jie1,2; Li, Xin1,2; Zhao, Li-Jing1,2; Gao, Li-Fang1,2; Yu, Hao1,2; Zhao, Xue-Jian1,2; Zhang, Ling1,2; Yang, Bao-Xue4
关键词: apoptosis ; hydroxyapatite ; prostate cancer ; RNA interference ; Stat3
刊名: ASIAN JOURNAL OF ANDROLOGY
发表日期: 2011-05-01
DOI: 10.1038/aja.2010.167
卷: 13, 期:3, 页:481-486
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Andrology ; Urology & Nephrology
研究领域[WOS]: Endocrinology & Metabolism ; Urology & Nephrology
关键词[WOS]: SMALL INTERFERING RNAS ; CELL-PROLIFERATION ; SIGNAL TRANSDUCER ; MAMMALIAN-CELLS ; DOWN-REGULATION ; DRUG-DELIVERY ; CANCER ; TRANSCRIPTION-3 ; EXPRESSION ; APOPTOSIS
英文摘要:

DNA vector-based Stat3-specific RNA interference (si-Stat3) blocks Stat3 signalling and inhibits prostate tumour growth. However, the antitumour activity depends on the efficient delivery of si-Stat3. The effects on the growth of mouse prostate cancer cells of si-Stat3 delivered by hydroxyapatite were determined in this study. RM-1 tumour blocks were transplanted into C57BL/6 mice. CaCl2-modified hydroxyapatite carrying si-Stat3 plasmids were injected into tumours, and tumour growth and histology were determined. The expression levels of Stat3, pTyr-Stat3, Bcl-2, Bax, Caspase3, VEGF and cyclin D1 were measured by western blot analysis. Amounts of apoptosis in cancer cells were analysed with immunohistochemistry and the terminal deoxyribonucleotidyl transferase-mediated dUTP-digoxigenin nick end-labelling (TUNEL) assay. The results showed that hydroxyapatite-delivered si-Stat3 significantly suppressed tumour growth up to 74% (P < 0.01). Stat3 expression was dramatically downregulated in the tumours. The immunohistochemistry and TUNEL results showed that si-Stat3-induced apoptosis (up to 42%, P < 0.01). The Stat3 downstream genes Bcl-2, VEGF and cyclin D1 were also strongly downregulated in the tumour tissues that also displayed significant increases in Bax expression and Caspase3 activity. These results suggest that hydroxyapatite can be used for the in vivo delivery of plasmid-based siRNAs into tumours. Asian Journal of Andrology (2011) 13, 481-486; doi: 10.1038/aja.2010.167; published online 7 February 2011

语种: 英语
所属项目编号: 30801354 ; 30970791 ; 30870921 ; 200810012 ; 20080154
项目资助者: National Natural Science Foundation of China ; Fundamental Research Funds for the Central Universities of China ; Jilin Provincial Science &amp ; Technology Department, China
WOS记录号: WOS:000290206800026
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/50834
Appears in Collections:基础医学院_药理学系_期刊论文

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作者单位: 1.Jilin Univ, Prostate Dis Prevent & Treatment Res Ctr, Changchun 130021, Peoples R China
2.Jilin Univ, Dept Pathophysiol, Norman Bethune Med Sch, Changchun 130021, Peoples R China
3.Jilin Univ, China Japan Union Hosp, Dept Emergency Med, Changchun 130033, Peoples R China
4.Peking Univ, Dept Pharmacol, Sch Basic Med Sci, Beijing 100191, Peoples R China

Recommended Citation:
Liang, Zuo-Wen,Guo, Bao-Feng,Li, Yang,et al. Plasmid-based Stat3 siRNA delivered by hydroxyapatite nanoparticles suppresses mouse prostate tumour growth in vivo[J]. ASIAN JOURNAL OF ANDROLOGY,2011,13(3):481-486.
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