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学科主题临床医学
A Tubing-Free Microfluidic Wound Healing Assay Enabling the Quantification of Vascular Smooth Muscle Cell Migration
Wei, Yuanchen1; Chen, Feng2; Zhang, Tao3; Chen, Deyong1; Jia, Xin2; Wang, Junbo1; Guo, Wei2; Chen, Jian1
刊名SCIENTIFIC REPORTS
2015-09-14
DOI10.1038/srep14049
5
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Multidisciplinary Sciences
研究领域[WOS]Science & Technology - Other Topics
关键词[WOS]SELF-ASSEMBLED MONOLAYERS ; GROWTH FACTOR-BB ; ANIMAL-MODELS ; MULTIPLE TYPES ; ATHEROSCLEROSIS ; CHIP ; PATHOGENESIS ; MECHANISMS ; CHANNELS ; CULTURE
英文摘要

This paper presents a tubing-free microfluidic wound healing assay to quantify the migration of vascular smooth muscle cells (VSMCs), where gravity was used to generate a laminar flow within microfluidic channels, enabling cell seeding, culture, and wound generation. As the first systemic study to quantify the migration of VSMCs within microfluidic environments, the effects of channel geometries, surface modifications and chemokines on cellular migration were investigated, revealing that 1) height of the micro channels had a significant impact on cell migration; 2) the surface coating of collagen induced more migration of VSMCs than fibronectin coated surfaces and 3) platelet derived growth factor resulted in maximal cell migration compared to tumor necrosis factor alpha and fetal bovine serum. Furthermore, migrations of five types of VSMCs (e.g., the human vascular smooth muscle cell line, two types of primary vascular smooth cells, and VSMCs isolated from two human samples) were quantified, finding that VSMCs from the cell line and human samples demonstrated comparable migration distances, which were significantly lower than the migration distances of two primary cell types. As a platform technology, this wound healing assay may function as a new model to study migration of VSMCs within microfluidic environments.

语种英语
WOS记录号WOS:000361149300001
项目编号2014CB744602 ; 2013T60950 ; 81261120561 ; 61431019 ; 61201077 ; 81370420 ; 2014AA093408
资助机构National Basic Research Program of China (973 Program) ; Special Financial Grant from the China Postdoctoral Science Foundation ; National Natural Science Foundation of China ; National High Technology Research and Development Program of China (863 Program) ; Beijing NOVA Program
引用统计
被引频次:6[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/50852
专题北京大学第二临床医学院_血管外科
作者单位1.Chinese Acad Sci, Inst Elect, State Key Lab Transducer Technol, Beijing 100190, Peoples R China
2.Chinese Peoples Liberat Army Gen Hosp, Clin Div Surg, Dept Vasc Surg, Beijing 100853, Peoples R China
3.Peking Univ Peoples Hosp, Dept Vasc Surg, Beijing 100044, Peoples R China
推荐引用方式
GB/T 7714
Wei, Yuanchen,Chen, Feng,Zhang, Tao,et al. A Tubing-Free Microfluidic Wound Healing Assay Enabling the Quantification of Vascular Smooth Muscle Cell Migration[J]. SCIENTIFIC REPORTS,2015,5.
APA Wei, Yuanchen.,Chen, Feng.,Zhang, Tao.,Chen, Deyong.,Jia, Xin.,...&Chen, Jian.(2015).A Tubing-Free Microfluidic Wound Healing Assay Enabling the Quantification of Vascular Smooth Muscle Cell Migration.SCIENTIFIC REPORTS,5.
MLA Wei, Yuanchen,et al."A Tubing-Free Microfluidic Wound Healing Assay Enabling the Quantification of Vascular Smooth Muscle Cell Migration".SCIENTIFIC REPORTS 5(2015).
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