IR@PKUHSC  > 北京大学临床肿瘤学院
学科主题临床医学
Expression of vascular endothelial growth factor and its receptors KDR and Flt-1 in gastric cancer cells
Zhang, H; Wu, J; Meng, L; Shou, CC
刊名WORLD JOURNAL OF GASTROENTEROLOGY
2002-12-01
8期:6页:994-998
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Gastroenterology & Hepatology
研究领域[WOS]Gastroenterology & Hepatology
关键词[WOS]HELICOBACTER-PYLORI INFECTION ; FACTOR VEGF ; AUTOCRINE ; RISK ; PARACRINE ; THERAPY ; MGC803 ; CHINA
英文摘要

AIM: The expression of vascular endothelial growth factor (VEGF) and its receptors KDR and Flt-1 by gastric carcinoma tissues and different gastric carcinoma cell lines was detected to elucidate the molecular mechanism of this growth factor in promoting tumor growth.

METHODS: The expression of VEGF, Flt-1 and KDR was determined by reverse transcription-polymerase chain reaction (RT-PCR) in gastric cancer cell lines RF-1, RF-48, AGS-1, NCI-N87, NCI-SNU-1, NCI-SNU-5, NCI-SNU-16 and KATO-III. The expression of Flt-1 and KDR in paraffin-embedded specimens of gastric cancer was determined by immunohistochemistry. The 3-(4,5-dimethylthiazol-2-yl)-2, 5-diphenyltetrazolium bromide (MTT) assay was used to assess the role of VEGF in tumor cell proliferation.

RESULTS: All 8 gastric cancer cell lines analyzed expressed VEGF(121) and VEGF(165) and six of them expressed both Flt-1 and KDR, while cell line NCI-SNU-5 expressed Flt-1 only and cell line KATOIII expressed neither Flt-1 nor KDR. The gastric carcinoma tissues expressed Flt-1 and KDR widely, with the positive rate of expression of Flt-1 and KDR being 84.6% and 70% respectively. The exogenous VEGF stimulated the growth of KDR-positive cell lines NCI-N87 and AGS-1 in a dose-dependent manner but exhibited no effect on the growth of KDR-negative cell line NCI-N87.

CONCLUSION: VEGF and its receptors KDR and Flt-1 were expressed widely in gastric carcinoma cells and the VEGF stimulated KDR-positive tumor cell growth directly. These results suggest that VEGF may play a role in promoting tumor growth and metastasis by participating in both paracrine and autocrine pathways.

语种英语
WOS记录号WOS:000179741700007
引用统计
被引频次:58[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/50854
专题北京大学临床肿瘤学院
作者单位Peking Univ, Sch Oncol, Beijing Inst Canc Res, Dept Biochem & Mol Biol, Beijing 100034, Peoples R China
推荐引用方式
GB/T 7714
Zhang, H,Wu, J,Meng, L,et al. Expression of vascular endothelial growth factor and its receptors KDR and Flt-1 in gastric cancer cells[J]. WORLD JOURNAL OF GASTROENTEROLOGY,2002,8(6):994-998.
APA Zhang, H,Wu, J,Meng, L,&Shou, CC.(2002).Expression of vascular endothelial growth factor and its receptors KDR and Flt-1 in gastric cancer cells.WORLD JOURNAL OF GASTROENTEROLOGY,8(6),994-998.
MLA Zhang, H,et al."Expression of vascular endothelial growth factor and its receptors KDR and Flt-1 in gastric cancer cells".WORLD JOURNAL OF GASTROENTEROLOGY 8.6(2002):994-998.
条目包含的文件
条目无相关文件。
个性服务
推荐该条目
保存到收藏夹
查看访问统计
导出为Endnote文件
谷歌学术
谷歌学术中相似的文章
[Zhang, H]的文章
[Wu, J]的文章
[Meng, L]的文章
百度学术
百度学术中相似的文章
[Zhang, H]的文章
[Wu, J]的文章
[Meng, L]的文章
必应学术
必应学术中相似的文章
[Zhang, H]的文章
[Wu, J]的文章
[Meng, L]的文章
相关权益政策
暂无数据
收藏/分享
所有评论 (0)
暂无评论
 

除非特别说明,本系统中所有内容都受版权保护,并保留所有权利。