|Expression of vascular endothelial growth factor and its receptors KDR and Flt-1 in gastric cancer cells|
|Zhang, H; Wu, J; Meng, L; Shou, CC|
|刊名||WORLD JOURNAL OF GASTROENTEROLOGY|
|WOS标题词||Science & Technology|
|类目[WOS]||Gastroenterology & Hepatology|
|研究领域[WOS]||Gastroenterology & Hepatology|
|关键词[WOS]||HELICOBACTER-PYLORI INFECTION ; FACTOR VEGF ; AUTOCRINE ; RISK ; PARACRINE ; THERAPY ; MGC803 ; CHINA|
AIM: The expression of vascular endothelial growth factor (VEGF) and its receptors KDR and Flt-1 by gastric carcinoma tissues and different gastric carcinoma cell lines was detected to elucidate the molecular mechanism of this growth factor in promoting tumor growth.
METHODS: The expression of VEGF, Flt-1 and KDR was determined by reverse transcription-polymerase chain reaction (RT-PCR) in gastric cancer cell lines RF-1, RF-48, AGS-1, NCI-N87, NCI-SNU-1, NCI-SNU-5, NCI-SNU-16 and KATO-III. The expression of Flt-1 and KDR in paraffin-embedded specimens of gastric cancer was determined by immunohistochemistry. The 3-(4,5-dimethylthiazol-2-yl)-2, 5-diphenyltetrazolium bromide (MTT) assay was used to assess the role of VEGF in tumor cell proliferation.
RESULTS: All 8 gastric cancer cell lines analyzed expressed VEGF(121) and VEGF(165) and six of them expressed both Flt-1 and KDR, while cell line NCI-SNU-5 expressed Flt-1 only and cell line KATOIII expressed neither Flt-1 nor KDR. The gastric carcinoma tissues expressed Flt-1 and KDR widely, with the positive rate of expression of Flt-1 and KDR being 84.6% and 70% respectively. The exogenous VEGF stimulated the growth of KDR-positive cell lines NCI-N87 and AGS-1 in a dose-dependent manner but exhibited no effect on the growth of KDR-negative cell line NCI-N87.
CONCLUSION: VEGF and its receptors KDR and Flt-1 were expressed widely in gastric carcinoma cells and the VEGF stimulated KDR-positive tumor cell growth directly. These results suggest that VEGF may play a role in promoting tumor growth and metastasis by participating in both paracrine and autocrine pathways.
|作者单位||Peking Univ, Sch Oncol, Beijing Inst Canc Res, Dept Biochem & Mol Biol, Beijing 100034, Peoples R China|
|Zhang, H,Wu, J,Meng, L,et al. Expression of vascular endothelial growth factor and its receptors KDR and Flt-1 in gastric cancer cells[J]. WORLD JOURNAL OF GASTROENTEROLOGY,2002,8(6):994-998.|
|APA||Zhang, H,Wu, J,Meng, L,&Shou, CC.(2002).Expression of vascular endothelial growth factor and its receptors KDR and Flt-1 in gastric cancer cells.WORLD JOURNAL OF GASTROENTEROLOGY,8(6),994-998.|
|MLA||Zhang, H,et al."Expression of vascular endothelial growth factor and its receptors KDR and Flt-1 in gastric cancer cells".WORLD JOURNAL OF GASTROENTEROLOGY 8.6(2002):994-998.|