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Injectable tissue-engineered bone composed of human adipose-derived stromal cells and platelet-rich plasma
Liu, Yunsong1; Zhou, Yongsheng1; Feng, Hailan1; Ma, Gui-e2; Ni, Yongwei1
关键词Bone Tissue Engineering Platelet-rich Plasma Adipose-derived Stromal Cells Cell Proliferation Cell Differentiation Injectable Bone
刊名BIOMATERIALS
2008-08-01
DOI10.1016/j.biomaterials.2008.04.037
29期:23页:3338-3345
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Engineering, Biomedical ; Materials Science, Biomaterials
研究领域[WOS]Engineering ; Materials Science
关键词[WOS]GROWTH-FACTOR ; IN-VITRO ; AUTOGENOUS BONE ; STEM-CELLS ; REGENERATION ; FIBROBLASTS ; SUBSTITUTE ; GRAFTS ; DIFFERENTIATION ; PROLIFERATION
英文摘要

This study aimed to evaluate the effects of human platelet-rich plasma (hPRP) on the proliferation and osteogenic differentiation of human adipose-derived stromal cells (hADSCs) and to construct a novel injectable tissue-engineered bone (ITB) composed of hPRP and hADSCs. hADSCs were isolated from liposuction tissues of healthy patients. hPRP was obtained by traditional two-step centrifugation. MTT, alkaline phosphatase (ALP) activity and mineralization assays were used to evaluate the effects of different concentrations of hPRP on cell proliferation and osteogenic differentiation in vitro. hADSCs cultured in optimal concentration of activated hPRP were subcutaneously injected into the inguinal groove of nude mice with hPRP and thrombin. X-ray, H&E staining and immunohistochemical analysis were used to examine the bone formation. Studies in vitro revealed that cell proliferation, ALP activity and mineralization were induced by hPRP and 10-12.5% of hPRP seemed to be the optimal concentration. Studies in vivo showed that this ITB formed bone structure in heterotopic site of nude mice. These findings indicate that the ITB composed of hPRP and hADSCs may represent a prologue for the development of a novel biological solution to bone defect. However, further investigations should be done to fully reveal the characteristics of this ITB. (c) 2008 Elsevier Ltd. All rights reserved.

语种英语
WOS记录号WOS:000257344600006
引用统计
被引频次:52[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/50859
专题北京大学口腔医学院_口腔修复科
作者单位1.Peking Univ, Dept Prosthodont, Sch & Hosp Stomatol, Beijing 100081, Peoples R China
2.Chinese Acad Med Sci, Plast Surg Hosp, Beijing 100041, Peoples R China
推荐引用方式
GB/T 7714
Liu, Yunsong,Zhou, Yongsheng,Feng, Hailan,et al. Injectable tissue-engineered bone composed of human adipose-derived stromal cells and platelet-rich plasma[J]. BIOMATERIALS,2008,29(23):3338-3345.
APA Liu, Yunsong,Zhou, Yongsheng,Feng, Hailan,Ma, Gui-e,&Ni, Yongwei.(2008).Injectable tissue-engineered bone composed of human adipose-derived stromal cells and platelet-rich plasma.BIOMATERIALS,29(23),3338-3345.
MLA Liu, Yunsong,et al."Injectable tissue-engineered bone composed of human adipose-derived stromal cells and platelet-rich plasma".BIOMATERIALS 29.23(2008):3338-3345.
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