北京大学医学部机构知识库
Advanced  
IR@PKUHSC  > 北京大学口腔医学院  > 口腔修复科  > 期刊论文
学科主题: 口腔医学
题名:
Injectable tissue-engineered bone composed of human adipose-derived stromal cells and platelet-rich plasma
作者: Liu, Yunsong1; Zhou, Yongsheng1; Feng, Hailan1; Ma, Gui-e2; Ni, Yongwei1
关键词: bone tissue engineering ; platelet-rich plasma ; adipose-derived stromal cells ; cell proliferation ; cell differentiation ; injectable bone
刊名: BIOMATERIALS
发表日期: 2008-08-01
DOI: 10.1016/j.biomaterials.2008.04.037
卷: 29, 期:23, 页:3338-3345
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Engineering, Biomedical ; Materials Science, Biomaterials
研究领域[WOS]: Engineering ; Materials Science
关键词[WOS]: GROWTH-FACTOR ; IN-VITRO ; AUTOGENOUS BONE ; STEM-CELLS ; REGENERATION ; FIBROBLASTS ; SUBSTITUTE ; GRAFTS ; DIFFERENTIATION ; PROLIFERATION
英文摘要:

This study aimed to evaluate the effects of human platelet-rich plasma (hPRP) on the proliferation and osteogenic differentiation of human adipose-derived stromal cells (hADSCs) and to construct a novel injectable tissue-engineered bone (ITB) composed of hPRP and hADSCs. hADSCs were isolated from liposuction tissues of healthy patients. hPRP was obtained by traditional two-step centrifugation. MTT, alkaline phosphatase (ALP) activity and mineralization assays were used to evaluate the effects of different concentrations of hPRP on cell proliferation and osteogenic differentiation in vitro. hADSCs cultured in optimal concentration of activated hPRP were subcutaneously injected into the inguinal groove of nude mice with hPRP and thrombin. X-ray, H&E staining and immunohistochemical analysis were used to examine the bone formation. Studies in vitro revealed that cell proliferation, ALP activity and mineralization were induced by hPRP and 10-12.5% of hPRP seemed to be the optimal concentration. Studies in vivo showed that this ITB formed bone structure in heterotopic site of nude mice. These findings indicate that the ITB composed of hPRP and hADSCs may represent a prologue for the development of a novel biological solution to bone defect. However, further investigations should be done to fully reveal the characteristics of this ITB. (c) 2008 Elsevier Ltd. All rights reserved.

语种: 英语
WOS记录号: WOS:000257344600006
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/50859
Appears in Collections:北京大学口腔医学院_口腔修复科_期刊论文

Files in This Item:

There are no files associated with this item.


作者单位: 1.Peking Univ, Dept Prosthodont, Sch & Hosp Stomatol, Beijing 100081, Peoples R China
2.Chinese Acad Med Sci, Plast Surg Hosp, Beijing 100041, Peoples R China

Recommended Citation:
Liu, Yunsong,Zhou, Yongsheng,Feng, Hailan,et al. Injectable tissue-engineered bone composed of human adipose-derived stromal cells and platelet-rich plasma[J]. BIOMATERIALS,2008,29(23):3338-3345.
Service
Recommend this item
Sava as my favorate item
Show this item's statistics
Export Endnote File
Google Scholar
Similar articles in Google Scholar
[Liu, Yunsong]'s Articles
[Zhou, Yongsheng]'s Articles
[Feng, Hailan]'s Articles
CSDL cross search
Similar articles in CSDL Cross Search
[Liu, Yunsong]‘s Articles
[Zhou, Yongsheng]‘s Articles
[Feng, Hailan]‘s Articles
Related Copyright Policies
Null
Social Bookmarking
Add to CiteULike Add to Connotea Add to Del.icio.us Add to Digg Add to Reddit

Items in IR are protected by copyright, with all rights reserved, unless otherwise indicated.

 

 

Valid XHTML 1.0!
Copyright © 2007-2017  北京大学医学部 - Feedback
Powered by CSpace