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学科主题: 基础医学
题名:
Elicitation of both CD4(+) and CD8(+) T-cell-mediated specific immune responses to HCA587 protein by autologous dendritic cells
作者: Li, B; He, X; Pang, X; Zhang, H; Chen, J; Chen, W
刊名: SCANDINAVIAN JOURNAL OF IMMUNOLOGY
发表日期: 2004-11-01
卷: 60, 期:5, 页:506-513
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Immunology
研究领域[WOS]: Immunology
关键词[WOS]: HEPATOCELLULAR-CARCINOMA ; ELISPOT ASSAY ; MAGE FAMILY ; EXPRESSION ; MELANOMA ; CANCER ; IMMUNOTHERAPY ; VACCINATION ; DIFFERENTIATION ; QUANTIFICATION
英文摘要:

We recently cloned a new member of cancer/testis antigen named HCA587, which was highly expressed in human hepatocellular carcinoma (HCC) tissues. To investigate it as a potential tumour-specific target for immunotherapy, the immunogenicity of this protein, especially the ability to induce specific cellular immune responses, was evaluated in the present study. As dendritic cells (DC) are the most potent antigen-presenting cells, DC-based vaccination has recently shown marked promise for the treatment of human malignancies by immunological intervention. Here, we demonstrate that autologous DC loaded with HCA587 protein could induce specific T-cell responses in healthy individuals by in vitro stimulations. Enzyme-linked immunospot analysis for interferon-gamma (IFN-gamma) secretion demonstrated HCA587-specific CD8(+) T cells in the antigen-stimulated peripheral blood lymphocytes, and the analysis of CD4(+) T cells by proliferation assay also showed antigen-specific reactivities in normal donors. Two-colour flow cytometric analysis of surface markers and intracellular cytokine expression demonstrated that HCA587-specific cytotoxic T lymphocytes exhibited a heterogeneous CD8(+)/CD56(+) expression, and a striking T-helper 1 cytokine bias (IFN-gamma(high)/IL-4(low)) was observed for both CD4(+) and CD8(+) HCA587-specific lymphocyte populations. We conclude that HCA587 is a potent immunogen that can induce CD4(+) and CD8(+) T-cell-mediated specific immune responses, and these findings propose HCA587 as a good candidate for the development of a therapeutic protein-based DC tumour vaccine for the treatment of HCC patients.

语种: 英语
WOS记录号: WOS:000225061900010
Citation statistics:
内容类型: 期刊论文
版本: 出版稿
URI标识: http://ir.bjmu.edu.cn/handle/400002259/50862
Appears in Collections:基础医学院_免疫学系_期刊论文

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作者单位: Peking Univ, Hlth Sci Ctr, Dept Immunol, Sch Basic Med Sci, Beijing 100083, Peoples R China

Recommended Citation:
Li, B,He, X,Pang, X,et al. Elicitation of both CD4(+) and CD8(+) T-cell-mediated specific immune responses to HCA587 protein by autologous dendritic cells[J]. SCANDINAVIAN JOURNAL OF IMMUNOLOGY,2004,60(5):506-513.
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