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Influence of lead (Pb2+) on the reactions of in vitro cultured rat aorta to 5-hydroxytryptamine
Zhang, LF1; Peng, SQ1; Wang, S1
关键词Lead Toxicity Aorta Vascular Reactivity 5-ht 5-ht2b Receptor Superoxide
刊名TOXICOLOGY LETTERS
2005-10-15
DOI10.1016/j.toxlet.2005.04.009
159期:1页:71-82
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Toxicology
研究领域[WOS]Toxicology
关键词[WOS]NITRIC-OXIDE SYNTHASE ; SOLUBLE GUANYLATE-CYCLASE ; BLOOD-PRESSURE ; INDUCED HYPERTENSION ; ENDOTHELIAL-CELLS ; MINERALOCORTICOID HYPERTENSION ; SUPEROXIDE-DISMUTASE ; VASCULAR WALL ; UP-REGULATION ; SEROTONIN
英文摘要

Although several studies demonstrated that lead induced abnormal vascular responses in low level lead exposed animals, investigations of the direct effects of lead on blood vessels are limited. In this study we tested the hypothesis that lead was able to directly affect the contractile reactivities of vessels. Male Wistar rat aortae were removed and cultured in PMRI 1640 with 1 ppm lead acetate for 0.5, 6, 12, 24 and 48 h, and then their responses to norepinephrine bitartrate (NE) and serotonin (5-hydroxytryptamine, 5-HT) were examined. The contractile responses to 5-HT of lead exposed aortae were significantly increased when the aortae were cultured for 24 and 48 It. Denudation of endothelium was able to abolish the increased contractile response completely. Diphenyleneiodonium (DPI), an inhibitor of the NAD(P)H oxidase, could abolish the increased contractile response to 5-HT. However, Vitamin C (VC) enhanced the contractile response of both groups to higher dosages of 5-HT. The expression of 5-HT2B receptor was not significantly altered by incubation with 1 ppm lead for 24 h. These data suggest that exposure to low levels of lead can directly increase the contraction of aorta to 5-HT. This effect is endothelium dependent, which is not mediated by increased expression of the 5-HT2B receptor. The increased contraction to 5-HT may be related to increased production of superoxide (O-2(center dot-)) induced by lead exposure. (c) 2005 Elsevier Ireland Ltd. All rights reserved.

语种英语
WOS记录号WOS:000232676700008
Citation statistics
Cited Times:5[WOS]   [WOS Record]     [Related Records in WOS]
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/50865
Collection北京大学公共卫生学院
作者单位1.Natl Beijing Ctr Drug Safety Evaluat & Res, Beijing Inst Pharmacol & Toxicol, Beijing 100850, Peoples R China
2.Peking Univ, Sch Publ Hlth, Hlth Sci Ctr, Dept Environm & Occupat Hlth, Beijing 100083, Peoples R China
Recommended Citation
GB/T 7714
Zhang, LF,Peng, SQ,Wang, S. Influence of lead (Pb2+) on the reactions of in vitro cultured rat aorta to 5-hydroxytryptamine[J]. TOXICOLOGY LETTERS,2005,159(1):71-82.
APA Zhang, LF,Peng, SQ,&Wang, S.(2005).Influence of lead (Pb2+) on the reactions of in vitro cultured rat aorta to 5-hydroxytryptamine.TOXICOLOGY LETTERS,159(1),71-82.
MLA Zhang, LF,et al."Influence of lead (Pb2+) on the reactions of in vitro cultured rat aorta to 5-hydroxytryptamine".TOXICOLOGY LETTERS 159.1(2005):71-82.
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