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学科主题: 口腔医学
题名:
Delivering MC3T3-E1 cells into injectable calcium phosphate cement through alginate-chitosan microcapsules for bone tissue engineering
作者: Qiao, Peng-yan1; Li, Fang-fang1; Dong, Li-min2; Xu, Tao3; Xie, Qiu-fei1,4
关键词: Injectable scaffold ; Calcium phosphate cement ; Osteoblast ; Microencapsulation ; Cell release ; Chitosan
刊名: JOURNAL OF ZHEJIANG UNIVERSITY-SCIENCE B
发表日期: 2014-04-01
DOI: 10.1631/jzus.B1300132
卷: 15, 期:4, 页:382-392
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Biochemistry & Molecular Biology ; Biotechnology & Applied Microbiology ; Medicine, Research & Experimental
研究领域[WOS]: Biochemistry & Molecular Biology ; Biotechnology & Applied Microbiology ; Research & Experimental Medicine
关键词[WOS]: STEM-CELLS ; FIBRIN MICROBEADS ; HYDROXYAPATITE CEMENT ; IN-VIVO ; SCAFFOLDS ; DIFFERENTIATION ; BEHAVIOR ; REPAIR ; PROLIFERATION ; BIOMATERIALS
英文摘要:

To deliver cells deep into injectable calcium phosphate cement (CPC) through alginate-chitosan (AC) microcapsules and investigate the biological behavior of the cells released from microcapsules into the CPC.

Mouse osteoblastic MC3T3-E1 cells were embedded in alginate and AC microcapsules using an electrostatic droplet generator. The two types of cell-encapsulating microcapsules were then mixed with a CPC paste. MC3T3-E1 cell viability was investigated using a Wst-8 kit, and osteogenic differentiation was demonstrated by an alkaline phosphatase (ALP) activity assay. Cell attachment in CPC was observed by an environment scanning electron microscopy.

Both alginate and AC microcapsules were able to release the encapsulated MC3T3-E1 cells when mixed with CPC paste. The released cells attached to the setting CPC scaffolds, survived, differentiated, and formed mineralized nodules. Cells grew in the pores concomitantly created by the AC microcapsules in situ within the CPC. At Day 21, cellular ALP activity in the AC group was approximately four times that at Day 7 and exceeded that of the alginate microcapsule group (P < 0.05). Pores formed by the AC microcapsules had a diameter of several hundred microns and were spherical compared with those formed by alginate microcapsules.

AC microcapsule is a promising carrier to release seeding cells deep into an injectable CPC scaffold for bone engineering.

语种: 英语
所属项目编号: 30772447 ; bmu2009139
项目资助者: National Natural Science Foundation of China ; Talent Introduction Project of Peking University Health Science Center, China
WOS记录号: WOS:000334578000009
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/50868
Appears in Collections:北京大学口腔医学院_口腔修复科_期刊论文

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作者单位: 1.Peking Univ, Sch & Hosp Stomatol, Dept Prosthodont, Beijing 100081, Peoples R China
2.Tsinghua Univ, Beijing Key Lab Fine Ceram, Inst Nucl & New Energy Technol, Beijing 100084, Peoples R China
3.Peking Univ, Sch & Hosp Stomatol, Dept Prevent Dent, Beijing 100081, Peoples R China
4.Peking Univ, Sch & Hosp Stomatol, Natl Engn Lab Digital & Mat Technol Stomatol, Beijing 100081, Peoples R China

Recommended Citation:
Qiao, Peng-yan,Li, Fang-fang,Dong, Li-min,et al. Delivering MC3T3-E1 cells into injectable calcium phosphate cement through alginate-chitosan microcapsules for bone tissue engineering[J]. JOURNAL OF ZHEJIANG UNIVERSITY-SCIENCE B,2014,15(4):382-392.
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