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学科主题: 基础医学
题名:
Enhanced cytoprotection and angiogenesis by bone marrow cell transplantation may contribute to improved ischemic myocardial function
作者: Zhang, SH1; Zhang, P1; Guo, JX1; Jia, ZQ1; Ma, KT1; Liu, YG1; Zhou, CY1; Li, LS1
关键词: cytoprotection ; angiogenesis ; ischemia ; stem cells ; transplantation
刊名: EUROPEAN JOURNAL OF CARDIO-THORACIC SURGERY
发表日期: 2004-02-01
DOI: 10.1016/j.ejcts.2003.10.021
卷: 25, 期:2, 页:188-195
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Cardiac & Cardiovascular Systems ; Respiratory System ; Surgery
研究领域[WOS]: Cardiovascular System & Cardiology ; Respiratory System ; Surgery
关键词[WOS]: HEAT-SHOCK PROTEINS ; NITRIC-OXIDE ; STEM-CELLS ; ISCHEMIA/REPERFUSION INJURY ; INFARCTED MYOCARDIUM ; RAT ; IMPLANTATION ; INDUCTION ; HEAT-SHOCK-PROTEIN-70 ; ANGIOBLASTS
英文摘要:

Objective: Heat shock proteins (HSPs) are cytoprotective proteins. Vascular endothelial growth factor (VEGF) is the most potent angiogenic factor. This study aimed to elucidate the possible role of cytoprotection and angiogenesis on cardiac function after bone marrow cell transplantation (BMT). Methods: Myocardial infarction was induced in inbred Lewis rats by left anterior descending artery ligation. A total of 5 x 10(6) bone marrow-mononuclear cells were transplanted into the ischemic zone by direct injection. At 1, 3, 7, 14 and 28 days post-transplantation, cardiac function was evaluated by echocardiography. The expressions of HSP32, HSP70 and VEGF were assessed by immunofluorescence and RT-PCR. The number of vessels was examined by immunohistochemistry. The differentiation of the transplanted cells was determined by immunofluorescence. Results: Echocardiography showed BMT led to sustained improvement in cardiac function, as assessed by left ventricle ejection fraction and fraction of shortening. Immunofluorescence revealed that the expressions of HSP32, HSP70 and VEGF were promoted in both transplanted bone marrow cells and recipient cardiomyocytes. RT-PCR showed that the mRNA expression levels of HSP32, HSP70 and VEGF in the BMT group were markedly higher in comparison with injection of peripheral blood cells or saline (P < 0.01) by day 7. Seven days later, the vessel count showed that angiogenesis had been induced to a significantly greater degree in the BMT groups. Fourteen days later, specific markers for myocardial or vascular endothelial cells were detected in the transplanted bone marrow cells. Conclusions: BMT upregulated the expressions of HSP32, HSP70 and VEGF in both transplanted bone marrow cells and recipient endogenous cardiomyocytes in the early phase post-transplantation. This enhanced cytoprotection and angiogenesis, and contributed to the functional recovery following cardiac infarction. In the late phase, the transplanted bone marrow cells might differentiate into both myocardial and vascular endothelial cells that enhanced the ischemic cardiac function further. (C) 2003 Elsevier B.V. All rights reserved.

语种: 英语
WOS记录号: WOS:000189040900009
Citation statistics:
内容类型: 期刊论文
版本: 出版稿
URI标识: http://ir.bjmu.edu.cn/handle/400002259/50873
Appears in Collections:基础医学院_北京大学干细胞研究中心_期刊论文

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作者单位: 1.Peking Univ, Stem Cell Res Ctr, Beijing 100083, Peoples R China
2.Peking Univ, Hosp 3, Dept Cardiol, Beijing 100083, Peoples R China
3.Peking Univ, Dept Biochem & Mol Biol, Sch Basic Med Sci, Beijing 100083, Peoples R China

Recommended Citation:
Zhang, SH,Zhang, P,Guo, JX,et al. Enhanced cytoprotection and angiogenesis by bone marrow cell transplantation may contribute to improved ischemic myocardial function[J]. EUROPEAN JOURNAL OF CARDIO-THORACIC SURGERY,2004,25(2):188-195.
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