学科主题基础医学
Enhanced cytoprotection and angiogenesis by bone marrow cell transplantation may contribute to improved ischemic myocardial function
Zhang, SH1; Zhang, P1; Guo, JX1; Jia, ZQ1; Ma, KT1; Liu, YG1; Zhou, CY1; Li, LS1
关键词cytoprotection angiogenesis ischemia stem cells transplantation
刊名EUROPEAN JOURNAL OF CARDIO-THORACIC SURGERY
2004-02-01
DOI10.1016/j.ejcts.2003.10.021
25期:2页:188-195
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Cardiac & Cardiovascular Systems ; Respiratory System ; Surgery
研究领域[WOS]Cardiovascular System & Cardiology ; Respiratory System ; Surgery
关键词[WOS]HEAT-SHOCK PROTEINS ; NITRIC-OXIDE ; STEM-CELLS ; ISCHEMIA/REPERFUSION INJURY ; INFARCTED MYOCARDIUM ; RAT ; IMPLANTATION ; INDUCTION ; HEAT-SHOCK-PROTEIN-70 ; ANGIOBLASTS
英文摘要

Objective: Heat shock proteins (HSPs) are cytoprotective proteins. Vascular endothelial growth factor (VEGF) is the most potent angiogenic factor. This study aimed to elucidate the possible role of cytoprotection and angiogenesis on cardiac function after bone marrow cell transplantation (BMT). Methods: Myocardial infarction was induced in inbred Lewis rats by left anterior descending artery ligation. A total of 5 x 10(6) bone marrow-mononuclear cells were transplanted into the ischemic zone by direct injection. At 1, 3, 7, 14 and 28 days post-transplantation, cardiac function was evaluated by echocardiography. The expressions of HSP32, HSP70 and VEGF were assessed by immunofluorescence and RT-PCR. The number of vessels was examined by immunohistochemistry. The differentiation of the transplanted cells was determined by immunofluorescence. Results: Echocardiography showed BMT led to sustained improvement in cardiac function, as assessed by left ventricle ejection fraction and fraction of shortening. Immunofluorescence revealed that the expressions of HSP32, HSP70 and VEGF were promoted in both transplanted bone marrow cells and recipient cardiomyocytes. RT-PCR showed that the mRNA expression levels of HSP32, HSP70 and VEGF in the BMT group were markedly higher in comparison with injection of peripheral blood cells or saline (P < 0.01) by day 7. Seven days later, the vessel count showed that angiogenesis had been induced to a significantly greater degree in the BMT groups. Fourteen days later, specific markers for myocardial or vascular endothelial cells were detected in the transplanted bone marrow cells. Conclusions: BMT upregulated the expressions of HSP32, HSP70 and VEGF in both transplanted bone marrow cells and recipient endogenous cardiomyocytes in the early phase post-transplantation. This enhanced cytoprotection and angiogenesis, and contributed to the functional recovery following cardiac infarction. In the late phase, the transplanted bone marrow cells might differentiate into both myocardial and vascular endothelial cells that enhanced the ischemic cardiac function further. (C) 2003 Elsevier B.V. All rights reserved.

语种英语
WOS记录号WOS:000189040900009
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被引频次:19[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
版本出版稿
条目标识符http://ir.bjmu.edu.cn/handle/400002259/50873
专题北京大学基础医学院_北京大学干细胞研究中心
北京大学基础医学院
北京大学第二临床医学院_胸外科
北京大学临床肿瘤学院_肿瘤放疗科
作者单位1.Peking Univ, Stem Cell Res Ctr, Beijing 100083, Peoples R China
2.Peking Univ, Hosp 3, Dept Cardiol, Beijing 100083, Peoples R China
3.Peking Univ, Dept Biochem & Mol Biol, Sch Basic Med Sci, Beijing 100083, Peoples R China
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GB/T 7714
Zhang, SH,Zhang, P,Guo, JX,et al. Enhanced cytoprotection and angiogenesis by bone marrow cell transplantation may contribute to improved ischemic myocardial function[J]. EUROPEAN JOURNAL OF CARDIO-THORACIC SURGERY,2004,25(2):188-195.
APA Zhang, SH.,Zhang, P.,Guo, JX.,Jia, ZQ.,Ma, KT.,...&Li, LS.(2004).Enhanced cytoprotection and angiogenesis by bone marrow cell transplantation may contribute to improved ischemic myocardial function.EUROPEAN JOURNAL OF CARDIO-THORACIC SURGERY,25(2),188-195.
MLA Zhang, SH,et al."Enhanced cytoprotection and angiogenesis by bone marrow cell transplantation may contribute to improved ischemic myocardial function".EUROPEAN JOURNAL OF CARDIO-THORACIC SURGERY 25.2(2004):188-195.
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