IR@PKUHSC  > 北京大学基础医学院  > 北京大学干细胞研究中心
学科主题基础医学
Enhanced cytoprotection and angiogenesis by bone marrow cell transplantation may contribute to improved ischemic myocardial function
Zhang, SH1; Zhang, P1; Guo, JX1; Jia, ZQ1; Ma, KT1; Liu, YG1; Zhou, CY1; Li, LS1
关键词cytoprotection angiogenesis ischemia stem cells transplantation
刊名EUROPEAN JOURNAL OF CARDIO-THORACIC SURGERY
2004-02-01
DOI10.1016/j.ejcts.2003.10.021
25期:2页:188-195
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Cardiac & Cardiovascular Systems ; Respiratory System ; Surgery
研究领域[WOS]Cardiovascular System & Cardiology ; Respiratory System ; Surgery
关键词[WOS]HEAT-SHOCK PROTEINS ; NITRIC-OXIDE ; STEM-CELLS ; ISCHEMIA/REPERFUSION INJURY ; INFARCTED MYOCARDIUM ; RAT ; IMPLANTATION ; INDUCTION ; HEAT-SHOCK-PROTEIN-70 ; ANGIOBLASTS
英文摘要

Objective: Heat shock proteins (HSPs) are cytoprotective proteins. Vascular endothelial growth factor (VEGF) is the most potent angiogenic factor. This study aimed to elucidate the possible role of cytoprotection and angiogenesis on cardiac function after bone marrow cell transplantation (BMT). Methods: Myocardial infarction was induced in inbred Lewis rats by left anterior descending artery ligation. A total of 5 x 10(6) bone marrow-mononuclear cells were transplanted into the ischemic zone by direct injection. At 1, 3, 7, 14 and 28 days post-transplantation, cardiac function was evaluated by echocardiography. The expressions of HSP32, HSP70 and VEGF were assessed by immunofluorescence and RT-PCR. The number of vessels was examined by immunohistochemistry. The differentiation of the transplanted cells was determined by immunofluorescence. Results: Echocardiography showed BMT led to sustained improvement in cardiac function, as assessed by left ventricle ejection fraction and fraction of shortening. Immunofluorescence revealed that the expressions of HSP32, HSP70 and VEGF were promoted in both transplanted bone marrow cells and recipient cardiomyocytes. RT-PCR showed that the mRNA expression levels of HSP32, HSP70 and VEGF in the BMT group were markedly higher in comparison with injection of peripheral blood cells or saline (P < 0.01) by day 7. Seven days later, the vessel count showed that angiogenesis had been induced to a significantly greater degree in the BMT groups. Fourteen days later, specific markers for myocardial or vascular endothelial cells were detected in the transplanted bone marrow cells. Conclusions: BMT upregulated the expressions of HSP32, HSP70 and VEGF in both transplanted bone marrow cells and recipient endogenous cardiomyocytes in the early phase post-transplantation. This enhanced cytoprotection and angiogenesis, and contributed to the functional recovery following cardiac infarction. In the late phase, the transplanted bone marrow cells might differentiate into both myocardial and vascular endothelial cells that enhanced the ischemic cardiac function further. (C) 2003 Elsevier B.V. All rights reserved.

语种英语
WOS记录号WOS:000189040900009
Citation statistics
Cited Times:18[WOS]   [WOS Record]     [Related Records in WOS]
文献类型期刊论文
版本出版稿
条目标识符http://ir.bjmu.edu.cn/handle/400002259/50873
Collection北京大学基础医学院_北京大学干细胞研究中心
作者单位1.Peking Univ, Stem Cell Res Ctr, Beijing 100083, Peoples R China
2.Peking Univ, Hosp 3, Dept Cardiol, Beijing 100083, Peoples R China
3.Peking Univ, Dept Biochem & Mol Biol, Sch Basic Med Sci, Beijing 100083, Peoples R China
Recommended Citation
GB/T 7714
Zhang, SH,Zhang, P,Guo, JX,et al. Enhanced cytoprotection and angiogenesis by bone marrow cell transplantation may contribute to improved ischemic myocardial function[J]. EUROPEAN JOURNAL OF CARDIO-THORACIC SURGERY,2004,25(2):188-195.
APA Zhang, SH.,Zhang, P.,Guo, JX.,Jia, ZQ.,Ma, KT.,...&Li, LS.(2004).Enhanced cytoprotection and angiogenesis by bone marrow cell transplantation may contribute to improved ischemic myocardial function.EUROPEAN JOURNAL OF CARDIO-THORACIC SURGERY,25(2),188-195.
MLA Zhang, SH,et al."Enhanced cytoprotection and angiogenesis by bone marrow cell transplantation may contribute to improved ischemic myocardial function".EUROPEAN JOURNAL OF CARDIO-THORACIC SURGERY 25.2(2004):188-195.
Files in This Item: Download All
File Name/Size DocType Version Access License
Enhanced cytoprotect(376KB)期刊论文出版稿开放获取CC BY-NC-SAView Download
Related Services
Recommend this item
Bookmark
Usage statistics
Export to Endnote
谷歌学术
谷歌学术Similar articles in
[Zhang, SH]'s Articles
[Zhang, P]'s Articles
[Guo, JX]'s Articles
百度学术
百度学术Similar articles in
[Zhang, SH]'s Articles
[Zhang, P]'s Articles
[Guo, JX]'s Articles
必应学术
必应学术Similar articles in
[Zhang, SH]'s Articles
[Zhang, P]'s Articles
[Guo, JX]'s Articles
Terms of Use
No data!
Social Bookmark/Share
File name: Enhanced cytoprotection and angiogenesis by bone marrow cell transplantation may contribute to improved ischemic myocardial function.pdf
Format: Adobe PDF
All comments (0)
No comment.
 

Items in the repository are protected by copyright, with all rights reserved, unless otherwise indicated.