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学科主题基础医学
Multiple variants and a differential splicing pattern of kinectin in human hepatocellular carcinoma
Wang, HC1; Su, YR1; Han, KJ1; Pang, XW1; Peng, JR1; Liang, B1; Wang, S1; Chen, WF1
关键词alternative splicing antibody response hepatocellular carcinoma kinectin serological analysis of recombinant cDNA expression library (SEREX)
刊名BIOCHEMISTRY AND CELL BIOLOGY-BIOCHIMIE ET BIOLOGIE CELLULAIRE
2004-04-01
DOI10.1139/004-003
82期:2页:321-327
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Biochemistry & Molecular Biology ; Cell Biology
研究领域[WOS]Biochemistry & Molecular Biology ; Cell Biology
关键词[WOS]KINESIN-BINDING PROTEIN ; CANCER/TESTIS ANTIGENS ; MOLECULAR-CLONING ; HUMAN-DISEASE ; CELL LINE ; IDENTIFICATION ; AUTOANTIBODIES ; MOTILITY ; CANCER ; TESTIS
英文摘要

To extend the search for hepatocellular carcinoma (HCC) associated antigens with immunogenicity for clinical applications. we constructed a cDNA expression library using resected human HCC tissue sample and screened it by serological analysis of recombinant cDNA expression library (SEREX) with autologous and allogeneic sera. A total of 24 distinct antigens were isolated and kinectin was the antigen most frequently identified. We found that kinectin was alternatively spliced at four sites and obtained all eight theoretical forms of variant, six by SEREX and two by RT-PCR, from the different splicing combinations of the last three sites. In addition, the splicing patterns of four sites were analyzed. Variant containing D2 was overexpressed in cancerous tissues and this alteration may be tumor associated. The four splicing sites. the variants generated by alternative splicing, and the humoral immune response in HCC patients, may help to analyze the role of kinectin in human HCC cell biology.

语种英语
WOS记录号WOS:000221223700007
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被引频次:14[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
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条目标识符http://ir.bjmu.edu.cn/handle/400002259/50880
专题北京大学基础医学院_免疫学系
北京大学医学部管理机构_医学部
北京大学第二临床医学院_胃肠外科
作者单位1.Peking Univ, Hlth Sci Ctr, Sch Basic Med Sci, Dept Immunol, Beijing 100083, Peoples R China
2.Peking Univ, Peoples Hosp, Ctr Hepatobiliary Surg, Beijing 100044, Peoples R China
3.Peking Univ, Peoples Hosp, Dept Surg 2, Beijing 100044, Peoples R China
4.Peking Univ, Peoples Hosp, Lab Surg Oncol, Beijing 100044, Peoples R China
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GB/T 7714
Wang, HC,Su, YR,Han, KJ,et al. Multiple variants and a differential splicing pattern of kinectin in human hepatocellular carcinoma[J]. BIOCHEMISTRY AND CELL BIOLOGY-BIOCHIMIE ET BIOLOGIE CELLULAIRE,2004,82(2):321-327.
APA Wang, HC.,Su, YR.,Han, KJ.,Pang, XW.,Peng, JR.,...&Chen, WF.(2004).Multiple variants and a differential splicing pattern of kinectin in human hepatocellular carcinoma.BIOCHEMISTRY AND CELL BIOLOGY-BIOCHIMIE ET BIOLOGIE CELLULAIRE,82(2),321-327.
MLA Wang, HC,et al."Multiple variants and a differential splicing pattern of kinectin in human hepatocellular carcinoma".BIOCHEMISTRY AND CELL BIOLOGY-BIOCHIMIE ET BIOLOGIE CELLULAIRE 82.2(2004):321-327.
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