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学科主题: 基础医学
题名:
Multiple variants and a differential splicing pattern of kinectin in human hepatocellular carcinoma
作者: Wang, HC1; Su, YR1; Han, KJ1; Pang, XW1; Peng, JR1; Liang, B1; Wang, S1; Chen, WF1
关键词: alternative splicing ; antibody response ; hepatocellular carcinoma ; kinectin ; serological analysis of recombinant cDNA expression library (SEREX)
刊名: BIOCHEMISTRY AND CELL BIOLOGY-BIOCHIMIE ET BIOLOGIE CELLULAIRE
发表日期: 2004-04-01
DOI: 10.1139/004-003
卷: 82, 期:2, 页:321-327
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Biochemistry & Molecular Biology ; Cell Biology
研究领域[WOS]: Biochemistry & Molecular Biology ; Cell Biology
关键词[WOS]: KINESIN-BINDING PROTEIN ; CANCER/TESTIS ANTIGENS ; MOLECULAR-CLONING ; HUMAN-DISEASE ; CELL LINE ; IDENTIFICATION ; AUTOANTIBODIES ; MOTILITY ; CANCER ; TESTIS
英文摘要:

To extend the search for hepatocellular carcinoma (HCC) associated antigens with immunogenicity for clinical applications. we constructed a cDNA expression library using resected human HCC tissue sample and screened it by serological analysis of recombinant cDNA expression library (SEREX) with autologous and allogeneic sera. A total of 24 distinct antigens were isolated and kinectin was the antigen most frequently identified. We found that kinectin was alternatively spliced at four sites and obtained all eight theoretical forms of variant, six by SEREX and two by RT-PCR, from the different splicing combinations of the last three sites. In addition, the splicing patterns of four sites were analyzed. Variant containing D2 was overexpressed in cancerous tissues and this alteration may be tumor associated. The four splicing sites. the variants generated by alternative splicing, and the humoral immune response in HCC patients, may help to analyze the role of kinectin in human HCC cell biology.

语种: 英语
WOS记录号: WOS:000221223700007
Citation statistics:
内容类型: 期刊论文
版本: 出版稿
URI标识: http://ir.bjmu.edu.cn/handle/400002259/50880
Appears in Collections:基础医学院_免疫学系_期刊论文

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作者单位: 1.Peking Univ, Hlth Sci Ctr, Sch Basic Med Sci, Dept Immunol, Beijing 100083, Peoples R China
2.Peking Univ, Peoples Hosp, Ctr Hepatobiliary Surg, Beijing 100044, Peoples R China
3.Peking Univ, Peoples Hosp, Dept Surg 2, Beijing 100044, Peoples R China
4.Peking Univ, Peoples Hosp, Lab Surg Oncol, Beijing 100044, Peoples R China

Recommended Citation:
Wang, HC,Su, YR,Han, KJ,et al. Multiple variants and a differential splicing pattern of kinectin in human hepatocellular carcinoma[J]. BIOCHEMISTRY AND CELL BIOLOGY-BIOCHIMIE ET BIOLOGIE CELLULAIRE,2004,82(2):321-327.
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