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学科主题: 药学
题名:
Chronic morphine administration induces over-expression of aldolase C with reduction of CREB phosphorylation in the mouse hippocampus
作者: Yang, Hai-Yu2; Pu, Xiao-Ping1,2
关键词: Morphine dependence ; Aldolase C ; CREB phosphorylation
刊名: EUROPEAN JOURNAL OF PHARMACOLOGY
发表日期: 2009-05-01
DOI: 10.1016/j.ejphar.2009.03.028
卷: 609, 期:1-3, 页:51-57
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Pharmacology & Pharmacy
研究领域[WOS]: Pharmacology & Pharmacy
关键词[WOS]: INDUCED PLACE PREFERENCE ; ELEMENT-BINDING PROTEIN ; OHMEFENTANYL STEREOISOMERS ; PHYSICAL-DEPENDENCE ; DORSAL HIPPOCAMPUS ; OPIATE WITHDRAWAL ; RAT HIPPOCAMPUS ; RECEPTOR ; MICE ; CAMP
英文摘要:

In recent studies, alterations in the activity and expression of metabolic enzymes, such as those involved in glycolysis, have been detected in morphine-dependent patients and animals. Increasing evidence demonstrates that the hippocampus is an important brain region associated with morphine dependence, but the molecular events occurring in the hippocampus following chronic exposure to morphine are poorly understood. Aldolase C is the brain-specific isoform of fructose-1, 6-bisphosphate aldolase which is a glycolytic enzyme catalyzing reactions in the glycolytic, gluconeogenic, and fructose metabolic pathways. Using Western blot and immunofluorescence assays, we found the expression of aldolase C was markedly increased in the mouse hippocampus following chronic morphine treatment. Naloxone pretreatment before morphine administration suppressed withdrawal jumping, weight loss, and overexpression of aldolase C. CREB is a transcription factor regulated through phosphorylation on Ser133, which is known to play a key role in the mechanism of morphine dependence. When detecting the expression of phosphorylated CREB (p-CREB) in the mouse hippocampus using Western blot and immunohistochemistry, we found CREB phosphorylation was clearly decreased following chronic morphine treatment. Interestingly, laser-confocal microscopy showed that overexpression of aldolase C in mouse hippocampal neurons was concomitant with the decreased immunoreactivity of p-CREB. The results suggest potential links between the morphine-induced alteration of aldolase C and the regulation of CREB phosphorylation, a possible mechanism of morphine dependence. (C) 2009 Elsevier B.V. All rights reserved.

语种: 英语
所属项目编号: 2004CB518902
项目资助者: National Program for Key Basic Research Projects of China
WOS记录号: WOS:000265968900009
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/50887
Appears in Collections:北京大学药学院_期刊论文

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作者单位: 1.Peking Univ, Dept Mol & Cellular Pharmacol, Sch Pharmaceut Sci, Beijing 100191, Peoples R China
2.Peking Univ, State Key Lab Nat & Biomimet Drugs, Beijing 100191, Peoples R China

Recommended Citation:
Yang, Hai-Yu,Pu, Xiao-Ping. Chronic morphine administration induces over-expression of aldolase C with reduction of CREB phosphorylation in the mouse hippocampus[J]. EUROPEAN JOURNAL OF PHARMACOLOGY,2009,609(1-3):51-57.
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