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Chronic morphine administration induces over-expression of aldolase C with reduction of CREB phosphorylation in the mouse hippocampus
Yang, Hai-Yu2; Pu, Xiao-Ping1,2
关键词Morphine Dependence Aldolase c Creb Phosphorylation
刊名EUROPEAN JOURNAL OF PHARMACOLOGY
2009-05-01
DOI10.1016/j.ejphar.2009.03.028
609期:1-3页:51-57
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Pharmacology & Pharmacy
研究领域[WOS]Pharmacology & Pharmacy
关键词[WOS]INDUCED PLACE PREFERENCE ; ELEMENT-BINDING PROTEIN ; OHMEFENTANYL STEREOISOMERS ; PHYSICAL-DEPENDENCE ; DORSAL HIPPOCAMPUS ; OPIATE WITHDRAWAL ; RAT HIPPOCAMPUS ; RECEPTOR ; MICE ; CAMP
英文摘要

In recent studies, alterations in the activity and expression of metabolic enzymes, such as those involved in glycolysis, have been detected in morphine-dependent patients and animals. Increasing evidence demonstrates that the hippocampus is an important brain region associated with morphine dependence, but the molecular events occurring in the hippocampus following chronic exposure to morphine are poorly understood. Aldolase C is the brain-specific isoform of fructose-1, 6-bisphosphate aldolase which is a glycolytic enzyme catalyzing reactions in the glycolytic, gluconeogenic, and fructose metabolic pathways. Using Western blot and immunofluorescence assays, we found the expression of aldolase C was markedly increased in the mouse hippocampus following chronic morphine treatment. Naloxone pretreatment before morphine administration suppressed withdrawal jumping, weight loss, and overexpression of aldolase C. CREB is a transcription factor regulated through phosphorylation on Ser133, which is known to play a key role in the mechanism of morphine dependence. When detecting the expression of phosphorylated CREB (p-CREB) in the mouse hippocampus using Western blot and immunohistochemistry, we found CREB phosphorylation was clearly decreased following chronic morphine treatment. Interestingly, laser-confocal microscopy showed that overexpression of aldolase C in mouse hippocampal neurons was concomitant with the decreased immunoreactivity of p-CREB. The results suggest potential links between the morphine-induced alteration of aldolase C and the regulation of CREB phosphorylation, a possible mechanism of morphine dependence. (C) 2009 Elsevier B.V. All rights reserved.

语种英语
WOS记录号WOS:000265968900009
项目编号2004CB518902
资助机构National Program for Key Basic Research Projects of China
引用统计
被引频次:16[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/50887
专题北京大学药学院
北京大学药学院_分子与细胞药理学系
作者单位1.Peking Univ, Dept Mol & Cellular Pharmacol, Sch Pharmaceut Sci, Beijing 100191, Peoples R China
2.Peking Univ, State Key Lab Nat & Biomimet Drugs, Beijing 100191, Peoples R China
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Yang, Hai-Yu,Pu, Xiao-Ping. Chronic morphine administration induces over-expression of aldolase C with reduction of CREB phosphorylation in the mouse hippocampus[J]. EUROPEAN JOURNAL OF PHARMACOLOGY,2009,609(1-3):51-57.
APA Yang, Hai-Yu,&Pu, Xiao-Ping.(2009).Chronic morphine administration induces over-expression of aldolase C with reduction of CREB phosphorylation in the mouse hippocampus.EUROPEAN JOURNAL OF PHARMACOLOGY,609(1-3),51-57.
MLA Yang, Hai-Yu,et al."Chronic morphine administration induces over-expression of aldolase C with reduction of CREB phosphorylation in the mouse hippocampus".EUROPEAN JOURNAL OF PHARMACOLOGY 609.1-3(2009):51-57.
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