北京大学医学部机构知识库
Advanced  
IR@PKUHSC  > 北京大学第二临床医学院  > 期刊论文
学科主题: 临床医学
题名:
Inhibitory effects on HLA-DR1-specific T-cell activation by influenza virus haemagglutinin-derived peptides
作者: Li, X1; Li, R1; Li, Z1
关键词: altered peptide ligands ; HLA-DR1 ; influenza virus haemagglutinin ; T-cell activation ; type II collagen
刊名: TISSUE ANTIGENS
发表日期: 2006
DOI: 10.1111/j.1399-0039.2005.00509.x
卷: 67, 期:1, 页:45-52
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Cell Biology ; Immunology ; Pathology
研究领域[WOS]: Cell Biology ; Immunology ; Pathology
关键词[WOS]: CLASS-II COMPLEXES ; HLA-DR MOLECULES ; RHEUMATOID-ARTHRITIS ; BINDING ; COLLAGEN ; LIGAND ; SUSCEPTIBILITY ; RESPONSES ; RECEPTOR ; MHC
英文摘要:

Collagen (CII) 263-272 peptide an autoantigen in rheumatoid arthritis is a,, specific human leukocyte antigen (HLA)-DR1/4-binding peptide recognized by T-cell receptors (TCR). The affinity of influenza virus haemagglutinin (HA) 306318 peptide for the antigen-binding groove of HLA-DR1/4 molecules is higher than that of CII263-272. The HLA-DR1/4-binding residues of HA306-318 are located in the region 308-317. Altered HA308-317 peptides with substitutions of TCR-contact residues may inhibit HLA-DR1/4-speciFic T-cell activation by blocking the antigen-binding site of HLA-DR1/4 molecules. To evaluate the role of altered HA308-317 peptides in HLA-DR1-restricted T-cell activation, we synthesized three altered HA308-317 peptides. The specific binding of altered HA308-317 peptides to HLA-DR1 molecules was examined using flow cytometry. Effects of altered HA308-317 peptides on HLA-DR1-specific T-cell hybridoma were studied by measuring T-cell proliferation and Surface expression of CD69 or CD25. The results showed that altered HA308-317 peptides were able to bind to HLA-DR1 molecules and competed with CII263-272 or wildtype HA308-317 peptide. Compared with wildtype CII263-272 or HA308-317, altered HA308-317 peptides did not Stimulate significant T-cell proliferation and CD69 or CD25 expression. Furthermore, the altered HA308-317 peptides inhibited HLADR1-specific T-cell activation induced by CII263-272 or wildtype HA308-317 peptide, which may suggest an effective therapeutic strategy in inhibition of HLA-DR1-specilic T-cell responses in autoimmunity.

语种: 英语
WOS记录号: WOS:000235035200005
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/50888
Appears in Collections:北京大学第二临床医学院_期刊论文

Files in This Item:

There are no files associated with this item.


作者单位: 1.Beijing Univ, Peoples Hosp, Dept Immunol & Rheumatol, Beijing 100044, Peoples R China
2.Dalian Univ Technol, Sch Med, Dept Immunol, Dalian 110066, Peoples R China

Recommended Citation:
Li, X,Li, R,Li, Z. Inhibitory effects on HLA-DR1-specific T-cell activation by influenza virus haemagglutinin-derived peptides[J]. TISSUE ANTIGENS,2006,67(1):45-52.
Service
Recommend this item
Sava as my favorate item
Show this item's statistics
Export Endnote File
Google Scholar
Similar articles in Google Scholar
[Li, X]'s Articles
[Li, R]'s Articles
[Li, Z]'s Articles
CSDL cross search
Similar articles in CSDL Cross Search
[Li, X]‘s Articles
[Li, R]‘s Articles
[Li, Z]‘s Articles
Related Copyright Policies
Null
Social Bookmarking
Add to CiteULike Add to Connotea Add to Del.icio.us Add to Digg Add to Reddit

Items in IR are protected by copyright, with all rights reserved, unless otherwise indicated.

 

 

Valid XHTML 1.0!
Copyright © 2007-2017  北京大学医学部 - Feedback
Powered by CSpace