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学科主题: 临床医学
题名:
Inhibitory effects on HLA-DR1-specific T-cell activation by influenza virus haemagglutinin-derived peptides
作者: Li, X1; Li, R1; Li, Z1
关键词: altered peptide ligands ; HLA-DR1 ; influenza virus haemagglutinin ; T-cell activation ; type II collagen
刊名: TISSUE ANTIGENS
发表日期: 2006
DOI: 10.1111/j.1399-0039.2005.00509.x
卷: 67, 期:1, 页:45-52
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Cell Biology ; Immunology ; Pathology
研究领域[WOS]: Cell Biology ; Immunology ; Pathology
关键词[WOS]: CLASS-II COMPLEXES ; HLA-DR MOLECULES ; RHEUMATOID-ARTHRITIS ; BINDING ; COLLAGEN ; LIGAND ; SUSCEPTIBILITY ; RESPONSES ; RECEPTOR ; MHC
英文摘要:

Collagen (CII) 263-272 peptide an autoantigen in rheumatoid arthritis is a,, specific human leukocyte antigen (HLA)-DR1/4-binding peptide recognized by T-cell receptors (TCR). The affinity of influenza virus haemagglutinin (HA) 306318 peptide for the antigen-binding groove of HLA-DR1/4 molecules is higher than that of CII263-272. The HLA-DR1/4-binding residues of HA306-318 are located in the region 308-317. Altered HA308-317 peptides with substitutions of TCR-contact residues may inhibit HLA-DR1/4-speciFic T-cell activation by blocking the antigen-binding site of HLA-DR1/4 molecules. To evaluate the role of altered HA308-317 peptides in HLA-DR1-restricted T-cell activation, we synthesized three altered HA308-317 peptides. The specific binding of altered HA308-317 peptides to HLA-DR1 molecules was examined using flow cytometry. Effects of altered HA308-317 peptides on HLA-DR1-specific T-cell hybridoma were studied by measuring T-cell proliferation and Surface expression of CD69 or CD25. The results showed that altered HA308-317 peptides were able to bind to HLA-DR1 molecules and competed with CII263-272 or wildtype HA308-317 peptide. Compared with wildtype CII263-272 or HA308-317, altered HA308-317 peptides did not Stimulate significant T-cell proliferation and CD69 or CD25 expression. Furthermore, the altered HA308-317 peptides inhibited HLADR1-specific T-cell activation induced by CII263-272 or wildtype HA308-317 peptide, which may suggest an effective therapeutic strategy in inhibition of HLA-DR1-specilic T-cell responses in autoimmunity.

语种: 英语
WOS记录号: WOS:000235035200005
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/50888
Appears in Collections:北京大学第二临床医学院_期刊论文

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作者单位: 1.Beijing Univ, Peoples Hosp, Dept Immunol & Rheumatol, Beijing 100044, Peoples R China
2.Dalian Univ Technol, Sch Med, Dept Immunol, Dalian 110066, Peoples R China

Recommended Citation:
Li, X,Li, R,Li, Z. Inhibitory effects on HLA-DR1-specific T-cell activation by influenza virus haemagglutinin-derived peptides[J]. TISSUE ANTIGENS,2006,67(1):45-52.
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