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学科主题基础医学
Effects of acetazolamide and anordiol on osmotic water permeability in AQP1-cRNA injected Xenopus oocytel
Ma, B1; Xiang, Y1; Mu, SM1; Li, T1; Yu, HM1; Li, XJ1
关键词Aquaporins Acetazolamide Anordiol Permeability Xenopus
刊名ACTA PHARMACOLOGICA SINICA
2004
25期:1页:90-97
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Chemistry, Multidisciplinary ; Pharmacology & Pharmacy
研究领域[WOS]Chemistry ; Pharmacology & Pharmacy
关键词[WOS]AQUAPORIN CHIP ; CHANNEL GENE ; PROTEIN ; MICE ; RAT
英文摘要

AIM: To study the effects of acetazolamide and anordiol on osmotic water permeability in aquaporin I (AQP1)-cRNA injected Xenopus oocyte and their mechanisms. METHODS: AQP1 gene constructed in pBluescript was transcripted into cRNA in vitro and then the cRNA was injected in Xenopus oocytes. The effects of acetazolamide and anordiol on the water transport function of AQP1 were observed by assaying the osmotic swelling of oocytes. In addition, their effects on protein expression of AQP1 were quantitatively investigated by Western blotting method. RESULTS: After incubation for 15 min or 72 h, acetazolamide, a carbonic anhydrase inhibitor, equally reduced the water permeability of AQP1-cRNA injected oocyte in a dose-dependent manner. After incubation for 72 h, anordiol, an antiestrogen with partial estrogenic activity, reduced the osmotic water permeability dose dependently as well; however, no discernable action was observed after incubation with anordiol for 15 min. The Western blotting analysis showed that acetazolamide did not influence the protein expression of AQP1. However, after incubation for 72 h with anordiol (10 mumol/L), the quantity of AQP1 in the oocyte membrane was decreased dramatically (P<0.05). CONCLUSION: Both acetazolamide and anordiol inhibited the osmotic water permeability of AQP1-RNA injected oocyte, but their mechanisms were different. Acetazolamide functionally inhibited the osmotic water permeability of AQP1, whereas anordiol primarily decreased the amount of AQP1 protein in the oocyte membrane.

语种英语
WOS记录号WOS:000187915700014
引用统计
被引频次:47[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/50903
专题北京大学基础医学院_药理学系
北京大学基础医学院
北京大学口腔医学院_院长办公室、党委办公室
北京大学临床肿瘤学院_生物诊断与治疗中心
作者单位1.Peking Univ, Sch Basic Med Sci, Dept Pharmacol, Beijing 100083, Peoples R China
2.Natl Res Inst Family Planning, Beijing 100081, Peoples R China
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GB/T 7714
Ma, B,Xiang, Y,Mu, SM,et al. Effects of acetazolamide and anordiol on osmotic water permeability in AQP1-cRNA injected Xenopus oocytel[J]. ACTA PHARMACOLOGICA SINICA,2004,25(1):90-97.
APA Ma, B,Xiang, Y,Mu, SM,Li, T,Yu, HM,&Li, XJ.(2004).Effects of acetazolamide and anordiol on osmotic water permeability in AQP1-cRNA injected Xenopus oocytel.ACTA PHARMACOLOGICA SINICA,25(1),90-97.
MLA Ma, B,et al."Effects of acetazolamide and anordiol on osmotic water permeability in AQP1-cRNA injected Xenopus oocytel".ACTA PHARMACOLOGICA SINICA 25.1(2004):90-97.
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