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学科主题: 口腔医学
题名:
Postnatal epithelium and mesenchyme stem/progenitor cells in bioengineered amelogenesis and dentinogenesis
作者: Jiang, Nan1,2; Zhou, Jian2; Chen, Mo2; Schiff, Michael D.2; Lee, Chang H.2; Kong, Kimi2; Embree, Mildred C.2; Zhou, Yanheng1; Mao, Jeremy J.2
关键词: Epithelial ; Mesenchymal ; Stem cells ; BMP ; Wnt ; Tooth regeneration
刊名: BIOMATERIALS
发表日期: 2014-02-01
DOI: 10.1016/j.biomaterials.2013.11.061
卷: 35, 期:7, 页:2172-2180
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Engineering, Biomedical ; Materials Science, Biomaterials
研究领域[WOS]: Engineering ; Materials Science
关键词[WOS]: NEURAL CREST CELLS ; STEM-CELLS ; MOUSE INCISORS ; TOOTH ; DIFFERENTIATION ; ROOT ; REGENERATION ; CROWN
英文摘要:

Rodent incisors provide a classic model for studying epithelial mesenchymal interactions in development. However, postnatal stem/progenitor cells in rodent incisors have not been exploited for tooth regeneration. Here, we characterized postnatal rat incisor epithelium and mesenchyme stem/progenitor cells and found that they formed enamel- and dentin-like tissues in vivo. Epithelium and mesenchyme cells were harvested separately from the apical region of postnatal 4-5 day rat incisors. Epithelial and mesenchymal phenotypes were confirmed by immunocytochemistry, CFU assay and/or multi-lineage differentiation. CK14+, Sox2+ and Lgr5+ epithelium stem cells from the cervical loop enhanced amelogenin and ameloblastin expression upon BMP4 or FGF3 stimulation, signifying their differentiation towards ameloblast-like cells, whereas mesenchyme stem/progenitor cells upon BMP4, BMP7 and Wnt3a treatment robustly expressed Dspp, a hallmark of odontoblastic differentiation. We then control-released microencapsulated BMP4, BMP7 and Wnt3a in transplants of epithelium and mesenchyme stem/progenitor cells in the renal capsule of athymic mice in vivo. Enamel and dentin-like tissues were generated in two integrated layers with specific expression of amelogenin and ameloblastin in the newly formed, de novo enamel-like tissue, and DSP in dentin-like tissue. These findings suggest that postnatal epithelium and mesenchyme stem/progenitor cells can be primed towards bioengineered tooth regeneration. (C) 2013 Elsevier Ltd. All rights reserved.

语种: 英语
所属项目编号: R01DE023112 ; RC2DE020767
项目资助者: NIH
WOS记录号: WOS:000331502300010
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/50908
Appears in Collections:北京大学口腔医学院_牙周科_期刊论文

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作者单位: 1.Columbia Univ, Med Ctr, CCR, New York, NY 10032 USA
2.Peking Univ, Sch & Hosp Stomatol, Dept Orthodont, Beijing 100081, Peoples R China

Recommended Citation:
Jiang, Nan,Zhou, Jian,Chen, Mo,et al. Postnatal epithelium and mesenchyme stem/progenitor cells in bioengineered amelogenesis and dentinogenesis[J]. BIOMATERIALS,2014,35(7):2172-2180.
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