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学科主题口腔医学
Postnatal epithelium and mesenchyme stem/progenitor cells in bioengineered amelogenesis and dentinogenesis
Jiang, Nan1,2; Zhou, Jian2; Chen, Mo2; Schiff, Michael D.2; Lee, Chang H.2; Kong, Kimi2; Embree, Mildred C.2; Zhou, Yanheng1; Mao, Jeremy J.2
关键词Epithelial Mesenchymal Stem Cells Bmp Wnt Tooth Regeneration
刊名BIOMATERIALS
2014-02-01
DOI10.1016/j.biomaterials.2013.11.061
35期:7页:2172-2180
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Engineering, Biomedical ; Materials Science, Biomaterials
研究领域[WOS]Engineering ; Materials Science
关键词[WOS]NEURAL CREST CELLS ; STEM-CELLS ; MOUSE INCISORS ; TOOTH ; DIFFERENTIATION ; ROOT ; REGENERATION ; CROWN
英文摘要

Rodent incisors provide a classic model for studying epithelial mesenchymal interactions in development. However, postnatal stem/progenitor cells in rodent incisors have not been exploited for tooth regeneration. Here, we characterized postnatal rat incisor epithelium and mesenchyme stem/progenitor cells and found that they formed enamel- and dentin-like tissues in vivo. Epithelium and mesenchyme cells were harvested separately from the apical region of postnatal 4-5 day rat incisors. Epithelial and mesenchymal phenotypes were confirmed by immunocytochemistry, CFU assay and/or multi-lineage differentiation. CK14+, Sox2+ and Lgr5+ epithelium stem cells from the cervical loop enhanced amelogenin and ameloblastin expression upon BMP4 or FGF3 stimulation, signifying their differentiation towards ameloblast-like cells, whereas mesenchyme stem/progenitor cells upon BMP4, BMP7 and Wnt3a treatment robustly expressed Dspp, a hallmark of odontoblastic differentiation. We then control-released microencapsulated BMP4, BMP7 and Wnt3a in transplants of epithelium and mesenchyme stem/progenitor cells in the renal capsule of athymic mice in vivo. Enamel and dentin-like tissues were generated in two integrated layers with specific expression of amelogenin and ameloblastin in the newly formed, de novo enamel-like tissue, and DSP in dentin-like tissue. These findings suggest that postnatal epithelium and mesenchyme stem/progenitor cells can be primed towards bioengineered tooth regeneration. (C) 2013 Elsevier Ltd. All rights reserved.

语种英语
WOS记录号WOS:000331502300010
项目编号R01DE023112 ; RC2DE020767
资助机构NIH
引用统计
被引频次:16[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/50908
专题北京大学口腔医学院_牙周科
北京大学口腔医学院_中心实验室
作者单位1.Columbia Univ, Med Ctr, CCR, New York, NY 10032 USA
2.Peking Univ, Sch & Hosp Stomatol, Dept Orthodont, Beijing 100081, Peoples R China
推荐引用方式
GB/T 7714
Jiang, Nan,Zhou, Jian,Chen, Mo,et al. Postnatal epithelium and mesenchyme stem/progenitor cells in bioengineered amelogenesis and dentinogenesis[J]. BIOMATERIALS,2014,35(7):2172-2180.
APA Jiang, Nan.,Zhou, Jian.,Chen, Mo.,Schiff, Michael D..,Lee, Chang H..,...&Mao, Jeremy J..(2014).Postnatal epithelium and mesenchyme stem/progenitor cells in bioengineered amelogenesis and dentinogenesis.BIOMATERIALS,35(7),2172-2180.
MLA Jiang, Nan,et al."Postnatal epithelium and mesenchyme stem/progenitor cells in bioengineered amelogenesis and dentinogenesis".BIOMATERIALS 35.7(2014):2172-2180.
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