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学科主题: 药学
题名:
Pharmacokinetics of honokiol after intravenous guttae in beagle dogs assessed using ultra-performance liquid chromatography-tandem mass spectrometry
作者: Liang, Yi1; Cui, Gang2; Wang, Xiaoxue2; Zhang, Wei3; An, Quan3; Lin, Zongtao1; Wang, Hong1; Chen, Shizhong1
关键词: honokiol ; pharmacokinetics ; UPLC-MS ; MS ; plasma ; beagle dog
刊名: BIOMEDICAL CHROMATOGRAPHY
发表日期: 2014-10-01
DOI: 10.1002/bmc.3179
卷: 28, 期:10, 页:1378-1383
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Biochemical Research Methods ; Biochemistry & Molecular Biology ; Chemistry, Analytical ; Pharmacology & Pharmacy
研究领域[WOS]: Biochemistry & Molecular Biology ; Chemistry ; Pharmacology & Pharmacy
关键词[WOS]: CEREBRAL-ISCHEMIA-REPERFUSION ; APOPTOSIS ; RADICALS ; MAGNOLOL ; PRODUCT ; SIGNALS ; RATS
英文摘要:

A simple, rapid and sensitive ultra-performance liquid chromatography-tandem mass spectrometry method was developed and validated for the determination of honokiol in beagle dog plasma after intravenous guttae. With addition of the internal standard magnolol, plasma samples were precipitated with methanol and separated on a Shim-pack XR-ODS II (2.0x100mm, 2.2 mu m) with isocratic elution of methanol and water (80:20) solution at a flow rate of 0.2mL/min. A good separation of honokiol was achieved within 3.5min. Quantification was performed on a Waters Quattro Premier XE triple quadrupole mass spectrometer with electrospray ionization inlet in the negative multiple reaction monitoring mode. Good linearity was obtained over the concentration range of 5.12-15580ng/mL (r(2)>0.998). Intra- and inter-day precisions were <13.10%, and accuracy ranged from 89.21 to 99.92%. The lower limit of quantification for honokiol was 5.12ng/mL, and honokiol was stable under various conditions (three freeze-thaw cycles, short-term temperature, post-preparative and long-term temperature conditions.). This validated method was successfully applied to the pharmacokinetic study of honokiol in dogs by intravenous guttae. Copyright (c) 2014 John Wiley & Sons, Ltd.

语种: 英语
所属项目编号: 2009ZX09102-146
项目资助者: Major Projects of National Science and Technology on New Drug Creation and Development
WOS记录号: WOS:000342801800012
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/50914
Appears in Collections:北京大学药学院_期刊论文

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作者单位: 1.Chinese Inst Radiat Protect, Taiyuan 030006, Peoples R China
2.Peking Univ, Sch Pharmaceut Sci, Beijing 100191, Peoples R China
3.China Japan Friendship Hosp, Dept Pharm, Beijing 100029, Peoples R China

Recommended Citation:
Liang, Yi,Cui, Gang,Wang, Xiaoxue,et al. Pharmacokinetics of honokiol after intravenous guttae in b !E ultra-performance liquid chromatography-tandem mass spectrometry[J]. BIOMEDICAL CHROMATOGRAPHY,2014,28(10):1378-1383.
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