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学科主题: 药学
题名:
A HPLC method for simultaneous determination of 5-aminoimidazole-4-carboxamide riboside and its active metabolite 5-aminoimidazole-4-carboxamide ribotide in tumor-bearing nude mice plasma and its application to pharmacokinetics study
作者: Cheng, Xiaoliang1,2; Guo, Liping3; Li, Zaiquan4; Li, Liang1,2; Zhou, Tianyan1,2; Lu, Wei1,2
关键词: 5-Aminoimidazole-4-carboxamide riboside ; 5-Aminoimidazole-4-carboxamide ribotide ; HPLC ; Solid phase extraction ; Column switching
刊名: JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES
发表日期: 2013-02-01
DOI: 10.1016/j.jchromb.2012.12.020
卷: 915, 页:64-70
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Biochemical Research Methods ; Chemistry, Analytical
研究领域[WOS]: Biochemistry & Molecular Biology ; Chemistry
关键词[WOS]: ADENOSINE-REGULATING AGENT ; PROTEIN-KINASE ; AICA-RIBOSIDE ; CANCER CELLS ; APOPTOSIS ; IDENTIFICATION ; CHROMATOGRAPHY ; PROLIFERATION ; ACTIVATION ; AMPK
英文摘要:

A HPLC method with on-line solid phase extraction (SPE) and column switching was developed for simultaneous determination of 5-aminoimidazole-4-carboxamide riboside (AICA riboside) and its active metabolite 5-aminoimidazole-4-carboxamide ribotide (AICA ribotide) in nude mice plasma. Plasma sample was deproteinized by adding a half volume of 10% trichloroacetic acid (TCA), and the resulting supernatant was extracted with diethyl ether to remove TCA. 50 mu l aqueous fraction was injected onto a WAX-1 SPE column, and AICA ribotide was trapped on the SPE column, while AICA riboside was eluted from the SPE column. The chromatographic separation of AICA riboside was achieved on CG16 column, and separation of AICA ribotide was performed on HILIC-10 and WAX-1 column. The columns temperature was maintained at 40 degrees C, and the optimal detection wavelength was 268 nm for both AICA riboside and AICA ribotide. The total analytical run time was 40 min. The proposed method was linear over the range of 0.1-500 mu g/ml for AICA riboside and 0.03-50 mu g/ml for AICA ribotide. The lower limit of quantification (LLOQ) was 100 and 30 ng/ml for AICA riboside and AICA ribotide, respectively. The sensitivity, accuracy and precision of this method were within acceptable limits during validation period. The method was successfully applied to investigate the pharmacokinetics characteristics of AICA riboside and its active metabolite AICA ribotide in nude mice bearing MCF-7 cell xenografts. (C) 2013 Elsevier B.V. All rights reserved.

语种: 英语
所属项目编号: BMU20110263E
项目资助者: Thermo Fisher Scientific Inc. ; Innovation Team of Ministry of Education
WOS记录号: WOS:000314559800011
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/50918
Appears in Collections:北京大学药学院_药剂学系_期刊论文

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作者单位: 1.Peking Univ, State Key Lab Nat & Biomimet Drugs, Hlth Sci Ctr, Beijing 100191, Peoples R China
2.Peking Univ, Sch Pharmaceut Sci, Dept Pharmaceut, Beijing 100191, Peoples R China
3.Thermo Fisher Sci Inc, Beijing 100080, Peoples R China
4.Peking Univ, Sch Basic Med Sci, Dept Radiat Med, Hlth Sci Ctr, Beijing 100191, Peoples R China

Recommended Citation:
Cheng, Xiaoliang,Guo, Liping,Li, Zaiquan,et al. A HPLC method for simultaneous determination of 5-aminoimidazole-4-carboxamide riboside and its active metabolite 5-aminoimidazole-4-carboxamide ribotide in tumor-bearing nude mice plasma and its application to pharmacokinetics study[J]. JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES,2013,915:64-70.
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