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学科主题临床医学
Differential Expression of the RANKL/RANK/OPG System Is Associated with Bone Metastasis in Human Non-Small Cell Lung Cancer
Peng, Xianbo; Guo, Wei; Ren, Tingting; Lou, Zhiyuan; Lu, Xinchang; Zhang, Shuai; Lu, Qunshan; Sun, Yifeng
刊名PLOS ONE
2013-03-13
DOI10.1371/journal.pone.0058361
8期:3
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Multidisciplinary Sciences
资助者National Natural Science Foundation of China ; National Natural Science Foundation of China
研究领域[WOS]Science & Technology - Other Topics
关键词[WOS]FACTOR-KAPPA-B ; HUMAN PROSTATE-CANCER ; RECEPTOR ACTIVATOR ; OSTEOCLAST DIFFERENTIATION ; OSTEOPROTEGERIN LIGAND ; RANK-FC ; POSSIBLE INVOLVEMENT ; OSTEOLYTIC LESIONS ; SOLID TUMORS ; IN-VITRO
英文摘要

Background: Human non-small cell lung cancer (NSCLC) patients exhibit a high propensity to develop skeletal metastasis, resulting in excessive osteolytic activity. The RANKL/RANK/OPG system, which plays a pivotal role in bone remodeling by regulating osteoclast formation and activity, is of potential interest in this context.

Materials and Methods: Reverse transcriptase polymerase chain reaction, western blotting, and immunohistochemical analysis were used to examine the expression of RANKL, RANK, and OPG in human NSCLC cell lines with different metastatic potentials, as well as in 52 primary NSCLC samples and 75 NSCLC bone metastasis samples. In primary NSCLC patients, the expression of these proteins was correlated with clinicopathological parameters. Recombinant human RANKL and transfected RANKL cDNA were added to the PAa cell line to evaluate the promoter action of RANKL during the process of metastasis in vitro and in vivo.

Results: Up-regulated RANKL, RANK, and OPG expression and increased RANKL:OPG ratio were detected in NSCLC cell lines and in tumor tissues with bone metastasis, and were correlated with higher metastatic potential. The metastatic potential of NSCLC in vitro and in vivo, including migration and invasion ability, was significantly enhanced by recombinant human RANKL and the transfection of RANKL cDNA, and was impaired after OPG was added. The increased expression of RANKL and OPG correlated with tumor stage, lymph node metastasis, and distant metastasis.

Conclusions: Differential expression of RANKL, RANK, and OPG is associated with the metastatic potential of human NSCLC to skeleton, raising the possibility that the RANKL/RANK/OPG system could be a therapeutic target for the treatment of metastatic NSCLC patients.

语种英语
所属项目编号30973020 ; 81001193
资助者National Natural Science Foundation of China ; National Natural Science Foundation of China
WOS记录号WOS:000316849200058
引用统计
被引频次:21[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/50928
专题北京大学第二临床医学院_骨肿瘤科
作者单位Peking Univ, Peoples Hosp, Musculoskeletal Tumor Ctr, Beijing 100871, Peoples R China
推荐引用方式
GB/T 7714
Peng, Xianbo,Guo, Wei,Ren, Tingting,et al. Differential Expression of the RANKL/RANK/OPG System Is Associated with Bone Metastasis in Human Non-Small Cell Lung Cancer[J]. PLOS ONE,2013,8(3).
APA Peng, Xianbo.,Guo, Wei.,Ren, Tingting.,Lou, Zhiyuan.,Lu, Xinchang.,...&Sun, Yifeng.(2013).Differential Expression of the RANKL/RANK/OPG System Is Associated with Bone Metastasis in Human Non-Small Cell Lung Cancer.PLOS ONE,8(3).
MLA Peng, Xianbo,et al."Differential Expression of the RANKL/RANK/OPG System Is Associated with Bone Metastasis in Human Non-Small Cell Lung Cancer".PLOS ONE 8.3(2013).
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