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学科主题: 临床医学
题名:
Differential Expression of the RANKL/RANK/OPG System Is Associated with Bone Metastasis in Human Non-Small Cell Lung Cancer
作者: Peng, Xianbo; Guo, Wei; Ren, Tingting; Lou, Zhiyuan; Lu, Xinchang; Zhang, Shuai; Lu, Qunshan; Sun, Yifeng
刊名: PLOS ONE
发表日期: 2013-03-13
DOI: 10.1371/journal.pone.0058361
卷: 8, 期:3
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Multidisciplinary Sciences
研究领域[WOS]: Science & Technology - Other Topics
关键词[WOS]: FACTOR-KAPPA-B ; HUMAN PROSTATE-CANCER ; RECEPTOR ACTIVATOR ; OSTEOCLAST DIFFERENTIATION ; OSTEOPROTEGERIN LIGAND ; RANK-FC ; POSSIBLE INVOLVEMENT ; OSTEOLYTIC LESIONS ; SOLID TUMORS ; IN-VITRO
英文摘要:

Background: Human non-small cell lung cancer (NSCLC) patients exhibit a high propensity to develop skeletal metastasis, resulting in excessive osteolytic activity. The RANKL/RANK/OPG system, which plays a pivotal role in bone remodeling by regulating osteoclast formation and activity, is of potential interest in this context.

Materials and Methods: Reverse transcriptase polymerase chain reaction, western blotting, and immunohistochemical analysis were used to examine the expression of RANKL, RANK, and OPG in human NSCLC cell lines with different metastatic potentials, as well as in 52 primary NSCLC samples and 75 NSCLC bone metastasis samples. In primary NSCLC patients, the expression of these proteins was correlated with clinicopathological parameters. Recombinant human RANKL and transfected RANKL cDNA were added to the PAa cell line to evaluate the promoter action of RANKL during the process of metastasis in vitro and in vivo.

Results: Up-regulated RANKL, RANK, and OPG expression and increased RANKL:OPG ratio were detected in NSCLC cell lines and in tumor tissues with bone metastasis, and were correlated with higher metastatic potential. The metastatic potential of NSCLC in vitro and in vivo, including migration and invasion ability, was significantly enhanced by recombinant human RANKL and the transfection of RANKL cDNA, and was impaired after OPG was added. The increased expression of RANKL and OPG correlated with tumor stage, lymph node metastasis, and distant metastasis.

Conclusions: Differential expression of RANKL, RANK, and OPG is associated with the metastatic potential of human NSCLC to skeleton, raising the possibility that the RANKL/RANK/OPG system could be a therapeutic target for the treatment of metastatic NSCLC patients.

语种: 英语
所属项目编号: 30973020 ; 81001193
项目资助者: National Natural Science Foundation of China
WOS记录号: WOS:000316849200058
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/50928
Appears in Collections:北京大学第二临床医学院_骨肿瘤科_期刊论文

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作者单位: Peking Univ, Peoples Hosp, Musculoskeletal Tumor Ctr, Beijing 100871, Peoples R China

Recommended Citation:
Peng, Xianbo,Guo, Wei,Ren, Tingting,et al. Differential Expression of the RANKL/RANK/OPG System Is Associated with Bone Metastasis in Human Non-Small Cell Lung Cancer[J]. PLOS ONE,2013,8(3).
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