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学科主题基础医学
Preparation and characterization of a monoclonal antibody against CKLF1 using DNA immunization with in vivo electroporation
Chen, YY1; Zhang, T1; Li, T1; Han, WL1; Zhang, YM1; Ma, DL1
刊名HYBRIDOMA
2005-12-01
24期:6页:305-308
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Biochemical Research Methods ; Biotechnology & Applied Microbiology ; Immunology
研究领域[WOS]Biochemistry & Molecular Biology ; Biotechnology & Applied Microbiology ; Immunology
关键词[WOS]CHEMOKINE-LIKE FACTOR-1 ; GENE ; GENERATION ; VIRUS ; CYTOKINE ; VACCINE
英文摘要

Chemokine-like factor 1 (CKLF1) is a newly cloned human cytokine from PHA-stimulated U937 cells in our laboratory, which belongs to a novel gene family and has at least three alternative RNA splicing forms: CKLF2, CKLF3, and CKLF4. Former studies demonstrated that CKLF1 has a chemotaxis effect on different leukocytes both in vitro and in vivo. It can also stimulate the proliferation of mouse skeletal muscle cells and bone marrow cells. This study was designed to generate CKLF1-specific monoclonal antibody (MAb) for further exploration of its structure and function. Mice were immunized intramuscularly with naked plasmid DNA encoding CKLF1 gene with in vivo electroporation. Hybridomas were generated by the fusion of the spleenocytes to Sp2/0 myeloma cells. One hybridoma cell line designated as M4, which is stable in secreting anti-CKLF1 MAb, was generated. It belongs to the IgG1 isotype and is specific for the unique C-terminal domain of CKLFs protein. The specificity of this antibody has been assessed by enzyme-linked immunosorbent assay (ELISA) and Western blot. Our results demonstrated that intramuscular injection of naked DNA encoding CKLF1 gene combined with in vivo electroporation is an effective and simple method to raise MAbs that can be used for basic and clinical research of CKLF1 protein.

语种英语
WOS记录号WOS:000233972000005
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被引频次:7[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
版本出版稿
条目标识符http://ir.bjmu.edu.cn/handle/400002259/50943
专题北京大学基础医学院_免疫学系
北京大学基础医学院
作者单位1.Peking Univ, Sch Basic Med Sci, Lab Med Immunol, Beijing 100083, Peoples R China
2.Peking Univ, Ctr Human Dis Genom, Beijing 100871, Peoples R China
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GB/T 7714
Chen, YY,Zhang, T,Li, T,et al. Preparation and characterization of a monoclonal antibody against CKLF1 using DNA immunization with in vivo electroporation[J]. HYBRIDOMA,2005,24(6):305-308.
APA Chen, YY,Zhang, T,Li, T,Han, WL,Zhang, YM,&Ma, DL.(2005).Preparation and characterization of a monoclonal antibody against CKLF1 using DNA immunization with in vivo electroporation.HYBRIDOMA,24(6),305-308.
MLA Chen, YY,et al."Preparation and characterization of a monoclonal antibody against CKLF1 using DNA immunization with in vivo electroporation".HYBRIDOMA 24.6(2005):305-308.
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