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学科主题: 基础医学
题名:
Preparation and characterization of a monoclonal antibody against CKLF1 using DNA immunization with in vivo electroporation
作者: Chen, YY1; Zhang, T1; Li, T1; Han, WL1; Zhang, YM1; Ma, DL1
刊名: HYBRIDOMA
发表日期: 2005-12-01
卷: 24, 期:6, 页:305-308
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Biochemical Research Methods ; Biotechnology & Applied Microbiology ; Immunology
研究领域[WOS]: Biochemistry & Molecular Biology ; Biotechnology & Applied Microbiology ; Immunology
关键词[WOS]: CHEMOKINE-LIKE FACTOR-1 ; GENE ; GENERATION ; VIRUS ; CYTOKINE ; VACCINE
英文摘要:

Chemokine-like factor 1 (CKLF1) is a newly cloned human cytokine from PHA-stimulated U937 cells in our laboratory, which belongs to a novel gene family and has at least three alternative RNA splicing forms: CKLF2, CKLF3, and CKLF4. Former studies demonstrated that CKLF1 has a chemotaxis effect on different leukocytes both in vitro and in vivo. It can also stimulate the proliferation of mouse skeletal muscle cells and bone marrow cells. This study was designed to generate CKLF1-specific monoclonal antibody (MAb) for further exploration of its structure and function. Mice were immunized intramuscularly with naked plasmid DNA encoding CKLF1 gene with in vivo electroporation. Hybridomas were generated by the fusion of the spleenocytes to Sp2/0 myeloma cells. One hybridoma cell line designated as M4, which is stable in secreting anti-CKLF1 MAb, was generated. It belongs to the IgG1 isotype and is specific for the unique C-terminal domain of CKLFs protein. The specificity of this antibody has been assessed by enzyme-linked immunosorbent assay (ELISA) and Western blot. Our results demonstrated that intramuscular injection of naked DNA encoding CKLF1 gene combined with in vivo electroporation is an effective and simple method to raise MAbs that can be used for basic and clinical research of CKLF1 protein.

语种: 英语
WOS记录号: WOS:000233972000005
Citation statistics:
内容类型: 期刊论文
版本: 出版稿
URI标识: http://ir.bjmu.edu.cn/handle/400002259/50943
Appears in Collections:基础医学院_免疫学系_期刊论文

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作者单位: 1.Peking Univ, Sch Basic Med Sci, Lab Med Immunol, Beijing 100083, Peoples R China
2.Peking Univ, Ctr Human Dis Genom, Beijing 100871, Peoples R China

Recommended Citation:
Chen, YY,Zhang, T,Li, T,et al. Preparation and characterization of a monoclonal antibody against CKLF1 using DNA immunization with in vivo electroporation[J]. HYBRIDOMA,2005,24(6):305-308.
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