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学科主题: 基础医学
题名:
Effects of taurine and homocysteine on calcium homeostasis and hydrogen peroxide and superoxide anions in rat myocardial mitochondria
作者: Chang, L1; Zhao, J1; Xu, JX1; Jiang, W1; Tang, CS1; Qi, YF1
关键词: homocysteine ; mitochondria ; taurine
刊名: CLINICAL AND EXPERIMENTAL PHARMACOLOGY AND PHYSIOLOGY
发表日期: 2004-04-01
卷: 31, 期:4, 页:237-243
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Pharmacology & Pharmacy ; Physiology
研究领域[WOS]: Pharmacology & Pharmacy ; Physiology
关键词[WOS]: VASCULAR SMOOTH-MUSCLE ; OXIDATIVE STRESS ; DISEASE ; HEART ; EXPRESSION ; H2O2 ; CA2+
英文摘要:

1. Taurine and homocysteine are metabolites of methionine. Hyperhomocysteinaemia is one of the risk factors for cardiovascular disease. Although taurine may be a cardiovascular cytoprotective substance, we hypothesized that it may antagonize the effects of homocysteine on myocardial mitochondrial function.

2. We studied the effects of taurine and homocysteine on [Ca-45] uptake, Ca2+-ATPase activity and generation of hydrogen peroxide and superoxide anions in vitro in rat isolated myocardial mitochondria.

3. Results showed that the inhibition of mitochondrial [Ca-45] uptake by homocysteine (0.1, 0.5 and 1.0 mmol/L) was concentration dependent. Taurine (5, 10 and 20 mmol/L) promoted [Ca-45] uptake in a concentration-dependent manner, as well as concentration dependently reducing the homocysteine (0.5 mmol/L)-induced inhibition of mitochondrial [Ca-45] uptake.

4. Homocysteine significantly inhibited mitochondrial Ca2+-ATPase activity, whereas taurine had a diphasic action on this activity. Taurine, at 5 and 10 mmol/L, increased Ca2+-ATPase activity (P < 0.01), but 20 mmol/L taurine inhibited Ca2+-ATPase activity (P < 0.05). Taurine attenuated the inhibitory effect of homocysteine on Ca2+-ATPase activity.

5. Homocysteine stimulated the generation of hydrogen peroxide and superoxide anions. Taurine had no effect on the generation of the anions, but inhibited their homocysteine-stimulated generation.

6. These results indicate that taurine and homocysteine have opposite effects in myocardial mitochondria with regard to [Ca-45] uptake, Ca2+-ATPase activity and the generation of hydrogen peroxide and superoxide anions. Our results may show an important mechanism for the cardiovascular protective effects of taurine.

语种: 英语
WOS记录号: WOS:000220486500008
Citation statistics:
内容类型: 期刊论文
版本: 出版稿
URI标识: http://ir.bjmu.edu.cn/handle/400002259/50949
Appears in Collections:基础医学院_生理学与病理生理学系_期刊论文

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作者单位: 1.First Hosp, Inst Cardiovasc Dis Res, Beijing, Peoples R China
2.Sci Acad China, Inst Biophys, Beijing, Peoples R China
3.Peking Univ, Hlth Sci Ctr, Dept Physiol, Beijing 100083, Peoples R China

Recommended Citation:
Chang, L,Zhao, J,Xu, JX,et al. Effects of taurine and homocysteine on calcium homeostasis and hydrogen peroxide and superoxide anions in rat myocardial mitochondria[J]. CLINICAL AND EXPERIMENTAL PHARMACOLOGY AND PHYSIOLOGY,2004,31(4):237-243.
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