IR@PKUHSC  > 北京大学深圳医院
学科主题临床医学
Whole exon 5 and intron 5 replaced by RHCE in D-Va(Hus)
Shao, CP1; Xiong, W1; Wang, W1
关键词Rhd Rhce D-va D-vi Sequencing Chinese
刊名JOURNAL OF HUMAN GENETICS
2004-02-01
DOI10.1007/s10038-003-0112-5
49期:2页:106-108
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Genetics & Heredity
研究领域[WOS]Genetics & Heredity
关键词[WOS]MONOCLONAL ANTI-D ; D-VA ; PHENOTYPES ; EPITOPES ; CHINESE ; CELLS
英文摘要

The D-Va(Hus) was previously investigated through cDNA analysis, which revealed an RHD-CE(5)-D hybrid allele. However, the 5′ and 3′ breakpoints remain unknown. In this article, gene recombinations between the RHD and RHCE alleles were investigated by a combination approach of a sequence-specific primer PCR (PCR-SSP) and an RHD full-length coding region sequencing method on two Chinese subjects with weak D phenotypes. The hybrid Rhesus box of each individual was also investigated through an established PCR-based method. As a result, two partial D phenotypes, D-Va(Hus) and D-VI type III, were identified, each carrying one hybrid RHD-CE-D allele. The two samples were also serotyped with Rh phontypes of DccEe and DCcee, respectively. Other sequencing analyses of the D-Va(Hus) sample showed that the sequence of intron 4 is identical with RHD, whereas the whole sequence of exon 5 and intron 5 is identical with RHCE except for seven polymorphisms in the intron 5. We may concluded that in the case of this Chinese D-Va(Hus), the whole exon 5 and complete intron 5 of a total segment of 1801 nucleotides were replaced by RHCE suggesting that the breakpoints of the replaced region are the 5′ end of the exon 5 and the 3′ end of the intron 5.

语种英语
WOS记录号WOS:000189284900008
引用统计
被引频次:4[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/50956
专题北京大学深圳医院
作者单位1.Shenzhen Blood Ctr, Futian 518035, Shenzhen, Peoples R China
2.Shenzhen Inst Transfus Med, Futian 518035, Shenzhen, Peoples R China
3.Peking Univ, Shenzhen Hosp, Shenzhen, Peoples R China
4.Edith Cowan Univ, Ctr Human Genet, Perth, WA, Australia
5.Chinese Acad Sci, Grad Sch, Dept Biol, Beijing, Peoples R China
6.Hong Kong Univ Sci & Technol, Peking Univ, Med Ctr, Shenzhen, Peoples R China
7.Peking Univ, Hlth Sci Ctr, Sch Publ Hlth, Beijing 100871, Peoples R China
推荐引用方式
GB/T 7714
Shao, CP,Xiong, W,Wang, W. Whole exon 5 and intron 5 replaced by RHCE in D-Va(Hus)[J]. JOURNAL OF HUMAN GENETICS,2004,49(2):106-108.
APA Shao, CP,Xiong, W,&Wang, W.(2004).Whole exon 5 and intron 5 replaced by RHCE in D-Va(Hus).JOURNAL OF HUMAN GENETICS,49(2),106-108.
MLA Shao, CP,et al."Whole exon 5 and intron 5 replaced by RHCE in D-Va(Hus)".JOURNAL OF HUMAN GENETICS 49.2(2004):106-108.
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