学科主题临床医学
GC/TOF-MS-based Analytical Method of Endogenous Metabolites for Mouse Serum
Wu Sheng-Ming1; Feng Bo2; Cheng Jian-Hua1; Li Hai-Jing1; Fang Jun-Jian1; Yan Xian-Zhong1; Wei Lai2; Dong Fang-Ting1
关键词Gas Chromatography/time Of Flight-mass Spectrometer (gc/tOf-ms) Oximation Silylation Metabolomics
刊名CHEMICAL JOURNAL OF CHINESE UNIVERSITIES-CHINESE
2012-06-10
DOI10.3969/j.issn.0251-0790.2012.06.013
33期:6页:1188-1194
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Chemistry, Multidisciplinary
研究领域[WOS]Chemistry
关键词[WOS]FULMINANT HEPATIC-FAILURE ; METABONOMIC ANALYSIS ; MASS-SPECTROMETRY ; BALB/C MICE ; URINE ; RAT ; PROFILES ; TOXICITY ; SYSTEM
英文摘要

A gas chromatography/time of flight mass spectrometer(GC/TOF-MS) method of metabonomic analysis for small mouse serum was established. With optimizing derivatization, 269 peaks of GC/TOF-MS chromatogram were detected. Among them, 46 peaks with the similarity over 800 were identified for endogenous metabolites. 18 endogenous metabolites were selected randomly to evaluate the precision and stability of the method, and 14 mixture standard of amino acids were selected for quantitative analysis. The results showed that linearities of the 14 mixture standards were good over the range of 2.78-113.20 ng/mu L, precision for intra-day and inter-day tests were satisfying, and the precision values were all below 15% for 18 endogenous metabolites. Since trimethyl-silyl (TMS) derivatization of monosaccharides often results in the formation of multiple peaks, oximation can resolve the problem smoothly. The results suggest that the method could be usefully applied to metabolomic studies, by data processing, biological markers can be identified and a new way can be presented for disease diagnosis.

语种中文
WOS记录号WOS:000305819100013
引用统计
被引频次:3[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/50964
专题北京大学第二临床医学院_北京大学肝病研究所
作者单位1.Natl Ctr Biomed Anal, Beijing 100850, Peoples R China
2.Peking Univ, Peoples Hosp, Inst Hepatol, Beijing 100044, Peoples R China
推荐引用方式
GB/T 7714
Wu Sheng-Ming,Feng Bo,Cheng Jian-Hua,et al. GC/TOF-MS-based Analytical Method of Endogenous Metabolites for Mouse Serum[J]. CHEMICAL JOURNAL OF CHINESE UNIVERSITIES-CHINESE,2012,33(6):1188-1194.
APA Wu Sheng-Ming.,Feng Bo.,Cheng Jian-Hua.,Li Hai-Jing.,Fang Jun-Jian.,...&Dong Fang-Ting.(2012).GC/TOF-MS-based Analytical Method of Endogenous Metabolites for Mouse Serum.CHEMICAL JOURNAL OF CHINESE UNIVERSITIES-CHINESE,33(6),1188-1194.
MLA Wu Sheng-Ming,et al."GC/TOF-MS-based Analytical Method of Endogenous Metabolites for Mouse Serum".CHEMICAL JOURNAL OF CHINESE UNIVERSITIES-CHINESE 33.6(2012):1188-1194.
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