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学科主题: 临床医学
题名:
GC/TOF-MS-based Analytical Method of Endogenous Metabolites for Mouse Serum
作者: Wu Sheng-Ming1; Feng Bo2; Cheng Jian-Hua1; Li Hai-Jing1; Fang Jun-Jian1; Yan Xian-Zhong1; Wei Lai2; Dong Fang-Ting1
关键词: Gas chromatography/time of flight-mass spectrometer (GC/TOF-MS) ; Oximation ; Silylation ; Metabolomics
刊名: CHEMICAL JOURNAL OF CHINESE UNIVERSITIES-CHINESE
发表日期: 2012-06-10
DOI: 10.3969/j.issn.0251-0790.2012.06.013
卷: 33, 期:6, 页:1188-1194
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Chemistry, Multidisciplinary
研究领域[WOS]: Chemistry
关键词[WOS]: FULMINANT HEPATIC-FAILURE ; METABONOMIC ANALYSIS ; MASS-SPECTROMETRY ; BALB/C MICE ; URINE ; RAT ; PROFILES ; TOXICITY ; SYSTEM
英文摘要:

A gas chromatography/time of flight mass spectrometer(GC/TOF-MS) method of metabonomic analysis for small mouse serum was established. With optimizing derivatization, 269 peaks of GC/TOF-MS chromatogram were detected. Among them, 46 peaks with the similarity over 800 were identified for endogenous metabolites. 18 endogenous metabolites were selected randomly to evaluate the precision and stability of the method, and 14 mixture standard of amino acids were selected for quantitative analysis. The results showed that linearities of the 14 mixture standards were good over the range of 2.78-113.20 ng/mu L, precision for intra-day and inter-day tests were satisfying, and the precision values were all below 15% for 18 endogenous metabolites. Since trimethyl-silyl (TMS) derivatization of monosaccharides often results in the formation of multiple peaks, oximation can resolve the problem smoothly. The results suggest that the method could be usefully applied to metabolomic studies, by data processing, biological markers can be identified and a new way can be presented for disease diagnosis.

语种: 中文
WOS记录号: WOS:000305819100013
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/50964
Appears in Collections:北京大学第二临床医学院_北京大学肝病研究所_期刊论文

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作者单位: 1.Natl Ctr Biomed Anal, Beijing 100850, Peoples R China
2.Peking Univ, Peoples Hosp, Inst Hepatol, Beijing 100044, Peoples R China

Recommended Citation:
Wu Sheng-Ming,Feng Bo,Cheng Jian-Hua,et al. GC/TOF-MS-based Analytical Method of Endogenous Metabolites for Mouse Serum[J]. CHEMICAL JOURNAL OF CHINESE UNIVERSITIES-CHINESE,2012,33(6):1188-1194.
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