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学科主题临床医学
Methylation of p16 CpG islands associated with malignant transformation of gastric dysplasia in a population-based study
Sun, Y1; Deng, DJ1; You, WC1; Bai, H1; Zhang, L1; Zhou, J1; Shen, L1; Ma, JL1; Xie, YQ1; Li, JY1
刊名CLINICAL CANCER RESEARCH
2004-08-01
10期:15页:5087-5093
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Oncology
研究领域[WOS]Oncology
关键词[WOS]PERFORMANCE LIQUID-CHROMATOGRAPHY ; TUMOR-SUPPRESSOR GENE ; DNA METHYLATION ; ABERRANT METHYLATION ; HIGH-RISK ; MYELODYSPLASTIC SYNDROMES ; HUMAN CANCERS ; HYPERMETHYLATION ; LESIONS ; PROGRESSION
英文摘要

Purpose: Inactivation of p16 by aberrant methylation of CpG islands is a frequent event in carcinomas and precancerous lesions of various organs, including the stomach. The aim of this study is to investigate the relationship between p16 methylation and malignant transformation of human gastric dysplasia (DYS) based on follow-up endoscopic screening in a high-risk population.

Experimental Design: Genomic DNA samples were extracted from paraffin blocks of gastric mucosal biopsies that were histopathologically diagnosed as low-grade DYS from patients who developed gastric carcinomas [GCs (n = 21)] and those that did not do so (n = 21) during 5 years of follow-up. The methylation status of p16 CpG islands of each sample was detected by methylation-specific PCR, denatured high-performance liquid chromatography, and sequencing.

Results: Aberrant p16 methylation was observed in 5 of 21 samples of DYS that progressed to GC but in 0 of 21 samples that did not progress to GC (P = 0.048, two-sided). Sequencing results confirmed that all CpG sites were methylated in the analyzed sequence from these five p16-methylated cases. Furthermore, p16 methylation was also observed in the five subsequent GCs. Unmethylated p16 CpG islands were detected in all of the samples without p16 methylation.

Conclusions: Our findings suggest p16 methylation is correlated with the malignant transformation of gastric DYS, and p16 methylation might be a useful biomarker for prediction of malignant potential of gastric DYS.

语种英语
WOS记录号WOS:000223257200023
引用统计
被引频次:52[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/50972
专题北京大学临床肿瘤学院
北京大学临床肿瘤学院_消化肿瘤内科
北京大学临床肿瘤学院_病因学研究室
北京大学临床肿瘤学院_流行病学研究室
北京大学临床肿瘤学院_党院办公室
作者单位1.Peking Univ, Sch Oncol, Beijing 100036, Peoples R China
2.Beijing Inst Canc Res, Beijing, Peoples R China
推荐引用方式
GB/T 7714
Sun, Y,Deng, DJ,You, WC,et al. Methylation of p16 CpG islands associated with malignant transformation of gastric dysplasia in a population-based study[J]. CLINICAL CANCER RESEARCH,2004,10(15):5087-5093.
APA Sun, Y.,Deng, DJ.,You, WC.,Bai, H.,Zhang, L.,...&Li, JY.(2004).Methylation of p16 CpG islands associated with malignant transformation of gastric dysplasia in a population-based study.CLINICAL CANCER RESEARCH,10(15),5087-5093.
MLA Sun, Y,et al."Methylation of p16 CpG islands associated with malignant transformation of gastric dysplasia in a population-based study".CLINICAL CANCER RESEARCH 10.15(2004):5087-5093.
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