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学科主题: 临床医学
题名:
Methylation of p16 CpG islands associated with malignant transformation of gastric dysplasia in a population-based study
作者: Sun, Y1; Deng, DJ1; You, WC1; Bai, H1; Zhang, L1; Zhou, J1; Shen, L1; Ma, JL1; Xie, YQ1; Li, JY1
刊名: CLINICAL CANCER RESEARCH
发表日期: 2004-08-01
卷: 10, 期:15, 页:5087-5093
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Oncology
研究领域[WOS]: Oncology
关键词[WOS]: PERFORMANCE LIQUID-CHROMATOGRAPHY ; TUMOR-SUPPRESSOR GENE ; DNA METHYLATION ; ABERRANT METHYLATION ; HIGH-RISK ; MYELODYSPLASTIC SYNDROMES ; HUMAN CANCERS ; HYPERMETHYLATION ; LESIONS ; PROGRESSION
英文摘要:

Purpose: Inactivation of p16 by aberrant methylation of CpG islands is a frequent event in carcinomas and precancerous lesions of various organs, including the stomach. The aim of this study is to investigate the relationship between p16 methylation and malignant transformation of human gastric dysplasia (DYS) based on follow-up endoscopic screening in a high-risk population.

Experimental Design: Genomic DNA samples were extracted from paraffin blocks of gastric mucosal biopsies that were histopathologically diagnosed as low-grade DYS from patients who developed gastric carcinomas [GCs (n = 21)] and those that did not do so (n = 21) during 5 years of follow-up. The methylation status of p16 CpG islands of each sample was detected by methylation-specific PCR, denatured high-performance liquid chromatography, and sequencing.

Results: Aberrant p16 methylation was observed in 5 of 21 samples of DYS that progressed to GC but in 0 of 21 samples that did not progress to GC (P = 0.048, two-sided). Sequencing results confirmed that all CpG sites were methylated in the analyzed sequence from these five p16-methylated cases. Furthermore, p16 methylation was also observed in the five subsequent GCs. Unmethylated p16 CpG islands were detected in all of the samples without p16 methylation.

Conclusions: Our findings suggest p16 methylation is correlated with the malignant transformation of gastric DYS, and p16 methylation might be a useful biomarker for prediction of malignant potential of gastric DYS.

语种: 英语
WOS记录号: WOS:000223257200023
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/50972
Appears in Collections:北京大学临床肿瘤学院_期刊论文

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作者单位: 1.Peking Univ, Sch Oncol, Beijing 100036, Peoples R China
2.Beijing Inst Canc Res, Beijing, Peoples R China

Recommended Citation:
Sun, Y,Deng, DJ,You, WC,et al. Methylation of p16 CpG islands associated with malignant transformation of gastric dysplasia in a population-based study[J]. CLINICAL CANCER RESEARCH,2004,10(15):5087-5093.
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