IR@PKUHSC  > 北京大学第一临床医学院  > 泌尿外科
学科主题临床医学
Interaction of hTCF4 by yeast two-hybrid system
Lin, GT1; Zhang, ZW1; Zang, T1; Xin, DQ1; Chang, ZJ1; Guo, YL1
关键词Wnt/frizzled Htcf4 Yeast Two-hybrid
刊名CHINESE SCIENCE BULLETIN
2000-11-01
45期:21页:1973-1977
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Multidisciplinary Sciences
研究领域[WOS]Science & Technology - Other Topics
英文摘要

To investigate the interaction of hTCF4, the yeast two-hybrid system has been used for testing the interaction of mutants of hTCF4 with themselves. Mutants of hTCF4(hTCF4 I) and hTCF4 II) have been obtained by polymerase chain reaction (PCR). Bait (hTCF4 I-pDBLeu and hTCF4 II-pDBLeu) and prey (hTCF4 I-pPC86 and hTCF4 II-pPC86) have been constructed by DNA recombination for yeast two-hybrid. Interaction of hTCF4 II with itself is found in the reverse yeast two-hybrid system (GIBCOBRL Co.). However, no interactions are found in hTCF4 II with hTCF4 I and in hTCF4 I with hTCF4 I. These results suggest that hTCF4 could interact with itself to form homodimer or homocopolymer and perform the transcriptional activating function through LZ or HLH motifs in nucleus of renal cell carcinoma.

语种英语
WOS记录号WOS:000165415400012
Citation statistics
Cited Times:1[WOS]   [WOS Record]     [Related Records in WOS]
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/50975
Collection北京大学第一临床医学院_泌尿外科
作者单位1.Beijing Med Univ, Inst Urol, Beijing 100034, Peoples R China
2.Beijing Med Univ, Dept Physiol, Beijing 100034, Peoples R China
3.Qinghua Univ, Dept Biol, Beijing 100084, Peoples R China
Recommended Citation
GB/T 7714
Lin, GT,Zhang, ZW,Zang, T,et al. Interaction of hTCF4 by yeast two-hybrid system[J]. CHINESE SCIENCE BULLETIN,2000,45(21):1973-1977.
APA Lin, GT,Zhang, ZW,Zang, T,Xin, DQ,Chang, ZJ,&Guo, YL.(2000).Interaction of hTCF4 by yeast two-hybrid system.CHINESE SCIENCE BULLETIN,45(21),1973-1977.
MLA Lin, GT,et al."Interaction of hTCF4 by yeast two-hybrid system".CHINESE SCIENCE BULLETIN 45.21(2000):1973-1977.
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