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学科主题: 临床医学
题名:
Exposure to cigarette smoke upregulates AP-1 activity and induces TNF-alpha overexpression in mouse lungs
作者: Li, Yu-Tao; He, Bei; Wang, Yu-Zhu
刊名: INHALATION TOXICOLOGY
发表日期: 2009-06-01
DOI: 10.1080/08958370802322596
卷: 21, 期:5-7, 页:641-647
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Toxicology
研究领域[WOS]: Toxicology
关键词[WOS]: NF-KAPPA-B ; OBSTRUCTIVE PULMONARY-DISEASE ; TRANSCRIPTION FACTOR ; CHROMATIN-STRUCTURE ; BRONCHIAL BIOPSIES ; INDUCED EMPHYSEMA ; OXIDATIVE STRESS ; GENE-EXPRESSION ; INDUCED SPUTUM ; CELLS
英文摘要:

Cigarette smoke-triggered inflammation is important in the pathophysiology of chronic obstructive pulmonary disease, and involves overexpression of many proinflammatory genes. Transcription factors regulating expression of inflammatory mediators may play a key role in characterizing the disease. To observe alterations in pulmonary function, observe pathological changes in lung tissues, and detect changes in transcriptional factors, mice were exposed to 30 days of cigarette smoke. Pulmonary function was measured by pressure sensors, and pathological changes were observed in lung tissue sections. Nuclear factor (NF)-kappa B and AP-1 activities were detected by electrophoretic mobility shift assay (EMSA). The levels of inflammatory mediators tumor necrosis factor (TNF)-alpha, interleukin (IL)-6, and IL-8 in bronchoalveolar lavage fluid (BALF) were measured using enzyme-linked immunosorbent assay (ELISA). Pulmonary function was not markedly decreased after 30 days of smoke exposure. Exposure to this regimen of cigarette smoke induced peribronchial and perivascular lymphocytic aggregates and parenchymal accumulation of macrophages in mice. EMSA demonstrated that smoke exposure enhanced activator protein (AP)-1 DNA binding activation, but only slightly changed NF-kappa B activation in mouse lung. Compared to the control group, smoke exposure induced a notable increase in TNF-alpha in BALF These data demonstrated that subacute smoke-triggered lung inflammation was accompanied by inflammatory cell influx, AP-1 activation, and proinflammatory gene overexpression in mouse lungs.

语种: 英语
项目资助者: National Natural Science Foundation Council (China)
WOS记录号: WOS:000268576900025
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/50987
Appears in Collections:北京大学第三临床医学院_呼吸内科_期刊论文

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作者单位: Peking Univ, Hosp 3, Dept Resp Med, Beijing, Peoples R China

Recommended Citation:
Li, Yu-Tao,He, Bei,Wang, Yu-Zhu. Exposure to cigarette smoke upregulates AP-1 activity and induces TNF-alpha overexpression in mouse lungs[J]. INHALATION TOXICOLOGY,2009,21(5-7):641-647.
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