IR@PKUHSC  > 北京大学第一临床医学院  > 皮肤性病科
学科主题临床医学
Immunoglobulin variable region gene analysis to the autoantibody-secreting B cells from tumors in association with paraneoplastic autoimmune multiorgan syndrome
Wang, Jing; Bu, Dingfang; Zhu, Xuejun
刊名INTERNATIONAL JOURNAL OF DERMATOLOGY
2007-11-01
46期:11页:1146-1154
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Dermatology
研究领域[WOS]Dermatology
关键词[WOS]CHRONIC LYMPHOCYTIC-LEUKEMIA ; V-H GENES ; HUMAN HEAVY ; PEMPHIGUS ; ANTIGEN ; ENVOPLAKIN ; ANTIBODY ; DISEASE ; PCR ; HYPERMUTATION
英文摘要

Objectives We have studied the role of lymphoproliferative tumors in the pathogenesis of autoimmune and the origin of the autoantibodies in PNP. Objectives of this study is to understand the characteristics of immunoglobulin genes of the B-cells isolated from the tumor which can produce auto-antibody.

Methods Total RNA of the tumor cells was then isolated and the mRNA was reversely transcribed into cDNA. V-H and V-L genes were amplified and cloned and their sequences were analyzed.

Results 49 V H genes (527 to 577 bp) and 36V(L) genes ( 445 to 454 bp) sequences were cloned from five tumors. All of the IgV(L) were mostly homologous to IGKV4-01 germ-line gene. The frequency of IgV(H) germ-line gene usage in a decreasing order was IGHV3-23 > IGHV3-9 > IGHV4-31 > IGHV4-59. There was more nucleotide changes occurred in complementary determining regions ( CDR) than those in the framework regions (FR) in the V-H and V-L of B cell clones. In both V-H and V-L, the probability (P) that the number of observed replacement mutations (R) in the CDRs would occur by random chance was low.

Conclusions The clones of B-lymphocytes in the tumors carrying possibly functional rearranged immunoglobulin heavy and light chain genes were isolated and there were high incidences of somatic mutations in CDRs and relatively conserved sequences in framework region.

语种英语
WOS记录号WOS:000250646200007
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被引频次:4[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/50993
专题北京大学第一临床医学院_皮肤性病科
作者单位Peking Univ, Dept Dermatol, First Hosp, Beijing 100034, Peoples R China
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Wang, Jing,Bu, Dingfang,Zhu, Xuejun. Immunoglobulin variable region gene analysis to the autoantibody-secreting B cells from tumors in association with paraneoplastic autoimmune multiorgan syndrome[J]. INTERNATIONAL JOURNAL OF DERMATOLOGY,2007,46(11):1146-1154.
APA Wang, Jing,Bu, Dingfang,&Zhu, Xuejun.(2007).Immunoglobulin variable region gene analysis to the autoantibody-secreting B cells from tumors in association with paraneoplastic autoimmune multiorgan syndrome.INTERNATIONAL JOURNAL OF DERMATOLOGY,46(11),1146-1154.
MLA Wang, Jing,et al."Immunoglobulin variable region gene analysis to the autoantibody-secreting B cells from tumors in association with paraneoplastic autoimmune multiorgan syndrome".INTERNATIONAL JOURNAL OF DERMATOLOGY 46.11(2007):1146-1154.
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