北京大学医学部机构知识库
Advanced  
IR@PKUHSC  > 北京大学第一临床医学院  > 皮肤性病科  > 期刊论文
学科主题: 临床医学
题名:
Immunoglobulin variable region gene analysis to the autoantibody-secreting B cells from tumors in association with paraneoplastic autoimmune multiorgan syndrome
作者: Wang, Jing; Bu, Dingfang; Zhu, Xuejun
刊名: INTERNATIONAL JOURNAL OF DERMATOLOGY
发表日期: 2007-11-01
卷: 46, 期:11, 页:1146-1154
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Dermatology
研究领域[WOS]: Dermatology
关键词[WOS]: CHRONIC LYMPHOCYTIC-LEUKEMIA ; V-H GENES ; HUMAN HEAVY ; PEMPHIGUS ; ANTIGEN ; ENVOPLAKIN ; ANTIBODY ; DISEASE ; PCR ; HYPERMUTATION
英文摘要:

Objectives We have studied the role of lymphoproliferative tumors in the pathogenesis of autoimmune and the origin of the autoantibodies in PNP. Objectives of this study is to understand the characteristics of immunoglobulin genes of the B-cells isolated from the tumor which can produce auto-antibody.

Methods Total RNA of the tumor cells was then isolated and the mRNA was reversely transcribed into cDNA. V-H and V-L genes were amplified and cloned and their sequences were analyzed.

Results 49 V H genes (527 to 577 bp) and 36V(L) genes ( 445 to 454 bp) sequences were cloned from five tumors. All of the IgV(L) were mostly homologous to IGKV4-01 germ-line gene. The frequency of IgV(H) germ-line gene usage in a decreasing order was IGHV3-23 > IGHV3-9 > IGHV4-31 > IGHV4-59. There was more nucleotide changes occurred in complementary determining regions ( CDR) than those in the framework regions (FR) in the V-H and V-L of B cell clones. In both V-H and V-L, the probability (P) that the number of observed replacement mutations (R) in the CDRs would occur by random chance was low.

Conclusions The clones of B-lymphocytes in the tumors carrying possibly functional rearranged immunoglobulin heavy and light chain genes were isolated and there were high incidences of somatic mutations in CDRs and relatively conserved sequences in framework region.

语种: 英语
WOS记录号: WOS:000250646200007
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/50993
Appears in Collections:北京大学第一临床医学院_皮肤性病科_期刊论文

Files in This Item:

There are no files associated with this item.


作者单位: Peking Univ, Dept Dermatol, First Hosp, Beijing 100034, Peoples R China

Recommended Citation:
Wang, Jing,Bu, Dingfang,Zhu, Xuejun. Immunoglobulin variable region gene analysis to the autoantibody-secreting B cells from tumors in association with paraneoplastic autoimmune multiorgan syndrome[J]. INTERNATIONAL JOURNAL OF DERMATOLOGY,2007,46(11):1146-1154.
Service
Recommend this item
Sava as my favorate item
Show this item's statistics
Export Endnote File
Google Scholar
Similar articles in Google Scholar
[Wang, Jing]'s Articles
[Bu, Dingfang]'s Articles
[Zhu, Xuejun]'s Articles
CSDL cross search
Similar articles in CSDL Cross Search
[Wang, Jing]‘s Articles
[Bu, Dingfang]‘s Articles
[Zhu, Xuejun]‘s Articles
Related Copyright Policies
Null
Social Bookmarking
Add to CiteULike Add to Connotea Add to Del.icio.us Add to Digg Add to Reddit
所有评论 (0)
暂无评论
 
评注功能仅针对注册用户开放,请您登录
您对该条目有什么异议,请填写以下表单,管理员会尽快联系您。
内 容:
Email:  *
单位:
验证码:   刷新
您在IR的使用过程中有什么好的想法或者建议可以反馈给我们。
标 题:
 *
内 容:
Email:  *
验证码:   刷新

Items in IR are protected by copyright, with all rights reserved, unless otherwise indicated.

 

 

Valid XHTML 1.0!
Copyright © 2007-2017  北京大学医学部 - Feedback
Powered by CSpace