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学科主题: 基础医学
题名:
Overexpression of chemokine-like factor 2 promotes the proliferation and survival of C2C12 skeletal muscle cells
作者: Xia, DL1; Li, XT1; Lou, YX1; Han, WL1; Ding, PG1; Zhang, YM1; Di, CH1; Song, QS1; Ma, DL1
关键词: chemokine-like factor 2 ; skeletal muscle ; proliferation ; differentiation ; C2C12 ; muscle regeneration
刊名: BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR CELL RESEARCH
发表日期: 2002-08-19
卷: 1591, 期:1-3, 页:163-173
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Biochemistry & Molecular Biology ; Cell Biology
研究领域[WOS]: Biochemistry & Molecular Biology ; Cell Biology
关键词[WOS]: GROWTH-FACTOR-I ; TRANSCRIPTION FACTOR MEF2C ; MYOGENIN GENE-EXPRESSION ; SIGNALING PATHWAY ; SATELLITE CELLS ; FACTOR MEF-2 ; MADS BOX ; DIFFERENTIATION ; INSULIN ; ACTIVATION
英文摘要:

Chemokine-like factor 1 (CKLF1) is a novel cytokine first cloned from U937 cells. It contains different splicing forms and has chemotactic effects on a wide spectrum of cells both in vitro and in vivo; it can also stimulate the regeneration of skeletal muscle cells in vivo, but the mechanism remains unclear. To probe the myogenesis function of CKLF2, which is the largest isoform of CKLFs, C2C12 murine myoblasts were stably transfected with human CKLF2 eukaryotic expression vector. Compared with control vector transfected C2C12 cells, CKLF2 overexpression causes accelerated myoblast proliferation as determined by cell counting and [H-3]TdR incorporation assays. In addition, CKLF2 overexpression also promotes cell differentiation, which was determined by higher expression levels of myogenin, creatine kinase, myosin and the accelerated myoblast fusion. Further analysis also indicates that CKLF2 could activate the transcription activity of the bHLH/MyoD and MEF2 families. Finally, DNA synthesis and myotube formation could also be promoted by growing C2C12 cells in conditioned media from CKLF2-transfected cells. These findings strongly suggest a role for human CKLF2 in regulation of skeletal muscle myogenesis. (C) 2002 Elsevier Science B.V. All rights reserved.

语种: 英语
WOS记录号: WOS:000177807100019
Citation statistics:
内容类型: 期刊论文
版本: 出版稿
URI标识: http://ir.bjmu.edu.cn/handle/400002259/50995
Appears in Collections:基础医学院_免疫学系_期刊论文

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作者单位: 1.Peking Univ, Sch Basic Med Sci, Lab Med Immunol, Beijing 100083, Peoples R China
2.Peking Univ, Ctr Human Dis Genom, Beijing 10083, Peoples R China

Recommended Citation:
Xia, DL,Li, XT,Lou, YX,et al. Overexpression of chemokine-like factor 2 promotes the proliferation and survival of C2C12 skeletal muscle cells[J]. BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR CELL RESEARCH,2002,1591(1-3):163-173.
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