IR@PKUHSC  > 北京大学基础医学院  > 免疫学系
学科主题基础医学
Overexpression of chemokine-like factor 2 promotes the proliferation and survival of C2C12 skeletal muscle cells
Xia, DL1; Li, XT1; Lou, YX1; Han, WL1; Ding, PG1; Zhang, YM1; Di, CH1; Song, QS1; Ma, DL1
关键词chemokine-like factor 2 skeletal muscle proliferation differentiation C2C12 muscle regeneration
刊名BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR CELL RESEARCH
2002-08-19
1591期:1-3页:163-173
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Biochemistry & Molecular Biology ; Cell Biology
研究领域[WOS]Biochemistry & Molecular Biology ; Cell Biology
关键词[WOS]GROWTH-FACTOR-I ; TRANSCRIPTION FACTOR MEF2C ; MYOGENIN GENE-EXPRESSION ; SIGNALING PATHWAY ; SATELLITE CELLS ; FACTOR MEF-2 ; MADS BOX ; DIFFERENTIATION ; INSULIN ; ACTIVATION
英文摘要

Chemokine-like factor 1 (CKLF1) is a novel cytokine first cloned from U937 cells. It contains different splicing forms and has chemotactic effects on a wide spectrum of cells both in vitro and in vivo; it can also stimulate the regeneration of skeletal muscle cells in vivo, but the mechanism remains unclear. To probe the myogenesis function of CKLF2, which is the largest isoform of CKLFs, C2C12 murine myoblasts were stably transfected with human CKLF2 eukaryotic expression vector. Compared with control vector transfected C2C12 cells, CKLF2 overexpression causes accelerated myoblast proliferation as determined by cell counting and [H-3]TdR incorporation assays. In addition, CKLF2 overexpression also promotes cell differentiation, which was determined by higher expression levels of myogenin, creatine kinase, myosin and the accelerated myoblast fusion. Further analysis also indicates that CKLF2 could activate the transcription activity of the bHLH/MyoD and MEF2 families. Finally, DNA synthesis and myotube formation could also be promoted by growing C2C12 cells in conditioned media from CKLF2-transfected cells. These findings strongly suggest a role for human CKLF2 in regulation of skeletal muscle myogenesis. (C) 2002 Elsevier Science B.V. All rights reserved.

语种英语
WOS记录号WOS:000177807100019
引用统计
被引频次:18[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
版本出版稿
条目标识符http://ir.bjmu.edu.cn/handle/400002259/50995
专题北京大学基础医学院_免疫学系
北京大学医学部管理机构_医学部
北京大学基础医学院
北京大学临床肿瘤学院_医学影像科
作者单位1.Peking Univ, Sch Basic Med Sci, Lab Med Immunol, Beijing 100083, Peoples R China
2.Peking Univ, Ctr Human Dis Genom, Beijing 10083, Peoples R China
推荐引用方式
GB/T 7714
Xia, DL,Li, XT,Lou, YX,et al. Overexpression of chemokine-like factor 2 promotes the proliferation and survival of C2C12 skeletal muscle cells[J]. BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR CELL RESEARCH,2002,1591(1-3):163-173.
APA Xia, DL.,Li, XT.,Lou, YX.,Han, WL.,Ding, PG.,...&Ma, DL.(2002).Overexpression of chemokine-like factor 2 promotes the proliferation and survival of C2C12 skeletal muscle cells.BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR CELL RESEARCH,1591(1-3),163-173.
MLA Xia, DL,et al."Overexpression of chemokine-like factor 2 promotes the proliferation and survival of C2C12 skeletal muscle cells".BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR CELL RESEARCH 1591.1-3(2002):163-173.
条目包含的文件
文件名称/大小 文献类型 版本类型 开放类型 使用许可
1-s2.0-S016748890200(463KB)期刊论文出版稿开放获取CC BY-NC-SA浏览 请求全文
个性服务
推荐该条目
保存到收藏夹
查看访问统计
导出为Endnote文件
谷歌学术
谷歌学术中相似的文章
[Xia, DL]的文章
[Li, XT]的文章
[Lou, YX]的文章
百度学术
百度学术中相似的文章
[Xia, DL]的文章
[Li, XT]的文章
[Lou, YX]的文章
必应学术
必应学术中相似的文章
[Xia, DL]的文章
[Li, XT]的文章
[Lou, YX]的文章
相关权益政策
暂无数据
收藏/分享
文件名: 1-s2.0-S0167488902002707-main.pdf
格式: Adobe PDF
此文件暂不支持浏览
所有评论 (0)
暂无评论
 

除非特别说明,本系统中所有内容都受版权保护,并保留所有权利。