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Development and validation of a rapid and sensitive liquid chromatography-tandem mass spectrometry method for benvitimod quantification in human plasma
Zhao, Libo1; Zhu, Baoying2; Chen, Xin1; Chen, Genghui3; Chen, Haibo4; Li, Yuzhen1; Jing, Shan1; Fang, Yi1
关键词Benvitimod Lc/esi/ms/ms Human Plasma Pharmacokinetics
刊名JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES
2012-02-15
DOI10.1016/j.jchromb.2011.12.026
885页:160-165
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Biochemical Research Methods ; Chemistry, Analytical
研究领域[WOS]Biochemistry & Molecular Biology ; Chemistry
关键词[WOS]PERFORMANCE
英文摘要

Benvitimod is a newly synthesized non-steroid small molecule being developed as a candidate drug for the treatment of inflammatory skin diseases. Here a rapid, sensitive and specific high performance liquid chromatography-tandem mass spectrometry (LC/ESI/MS/MS) method was developed for the determination of benvitimod in human plasma. The samples were alkalified with disodium tetraborate firstly, and then extracted by methyl tert-butyl ether. Fluorophenyl-benvitimod was used as internal standard (IS.). Chromatographic separation was performed on an Ultra C-18 column (150 mm x 2.1 mm, 5.0 mu m). The mixed mobile phase delivered at 300 mu l/min was CH3CN/H2O, 76.65:23.35 (v/v), containing 0.2 mmol/L NH4COOH. Detection and quantitation was performed by electrospray ionization (ESI) and multiple reaction monitoring (MRM) in the negative ion mode. The most intense [M-H](-) MRM transition of benvitimod at m/z 253.1 -> 211.0 was used for benvitimod quantitation and the transition at m/z 270.9 -> 229.2 was used to monitor I.S. The calibration curve was linear within the concentration range of 0.1-10.0 ng/mL (r > 0.99). The lower limit of quantification (LLOQ) was 0.1 ng/mL. The extraction recovery was above 80%. The accuracy expressed as relative error (RE) was less than 1.03%. The intra- and inter-day precisions were less than 11.81%. The freeze-thaw stability was also investigated and it was found that both benvitimod and the 1.5. were quite stable. This method is especially useful for the pharmacokinetic study of benvitimod. (C) 2012 Elsevier B.V. All rights reserved.

语种英语
WOS记录号WOS:000301563400021
引用统计
被引频次:5[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/51027
专题北京大学第二临床医学院_药剂科
作者单位1.Peking Univ Peoples Hosp, Dept Pharm, Beijing 100044, Peoples R China
2.Guiyang Med Coll, Guiyang 550004, Peoples R China
3.Celestial Pharmaceut Co Ltd, Shenzhen 518057, Peoples R China
4.Xuzhou Med Coll, Xuzhou 221004, Peoples R China
推荐引用方式
GB/T 7714
Zhao, Libo,Zhu, Baoying,Chen, Xin,et al. Development and validation of a rapid and sensitive liquid chromatography-tandem mass spectrometry method for benvitimod quantification in human plasma[J]. JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES,2012,885:160-165.
APA Zhao, Libo.,Zhu, Baoying.,Chen, Xin.,Chen, Genghui.,Chen, Haibo.,...&Fang, Yi.(2012).Development and validation of a rapid and sensitive liquid chromatography-tandem mass spectrometry method for benvitimod quantification in human plasma.JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES,885,160-165.
MLA Zhao, Libo,et al."Development and validation of a rapid and sensitive liquid chromatography-tandem mass spectrometry method for benvitimod quantification in human plasma".JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES 885(2012):160-165.
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