|Immunophenotypic analysis of abnormal plasma cell clones in bone marrow of primary systemic light chain amyloidosis patients|
|Hu Yang1; Wang Mangju1; Chen Yan2; Chen Xue1; Fang Fang1; Liu Shiqin1; Zhang Ying1; Wu Xueqiang2; Zhu Ping1|
|关键词||Primary Systemic Light Chain Amyloidosis Plasma Cell Clone Light Chain Restriction Immunophenotype Flow Cytometry|
|刊名||CHINESE MEDICAL JOURNAL|
|WOS标题词||Science & Technology|
|类目[WOS]||Medicine, General & Internal|
|研究领域[WOS]||General & Internal Medicine|
|关键词[WOS]||MULTIPLE-MYELOMA ; FLOW-CYTOMETRY ; MONOCLONAL GAMMOPATHY ; AL AMYLOIDOSIS ; DIAGNOSIS ; IMMUNOGLOBULIN ; EXPRESSION ; PROGNOSIS ; LEUKEMIA ; UPDATE|
Background Primary systemic light chain amyloidosis (AL) is a rare plasma cell disease, our purpose was to analyze the immunophenotypic characteristics of the plasma cells in bone marrow in AL patients, and explore whether the detection of abnormal plasma cell clones in bone marrow by flow cytometry (FCM) could be used as an important indicator of AL diagnosis.
Methods Fresh bone marrow samples were collected from 51 AL, 21 multiple myeloma (MM), and 5 Waldenstrom′s macroglobulinemia (WM) patients. The immunophenotype of bone marrow cells were analyzed and compared by FCM using a panel of antibodies including CD45, CD38, CD138, CD117, CD56, and CD19.
Results In AL, light chain restriction could be identified in 31 cases (60.9%), in which the A light chain restriction was found in 24 cases (77.4%). In MM, K light chain restriction was found in 13 cases (61.9%), and A light chain restriction in eight cases. CD45 on abnormal plasma cells was negative to weakly positive in both AL and MM, but was positive to strongly positive in WM. In the bone marrow plasma cells of the 51 AL, 78.4% were CD56+, 68.6% were CD117+, and 88.2% were CD19- While in the 21 MM cases, 66.7% were CD56+, 38.1% were CD117+, and 90.4% were CD19. The plasmacytoid lymphocytes in the five WM patients were CD19+ and CD56-, CD117-.
Conclusion Detection of abnormal plasma cell clones in bone marrow by FCM is valuable for the diagnosis of AL.
|项目编号||81370612 ; 81102211|
|资助机构||National Natural Science Foundation of China|
|作者单位||1.Peking Univ, Hosp 1, Dept Hematol, Beijing 100034, Peoples R China|
2.Beijing Aerosp Gen Hosp, Inst Hematol & Oncol, Beijing 100076, Peoples R China
|Hu Yang,Wang Mangju,Chen Yan,et al. Immunophenotypic analysis of abnormal plasma cell clones in bone marrow of primary systemic light chain amyloidosis patients[J]. CHINESE MEDICAL JOURNAL,2014,127(15):2765-2770.|
|APA||Hu Yang.,Wang Mangju.,Chen Yan.,Chen Xue.,Fang Fang.,...&Zhu Ping.(2014).Immunophenotypic analysis of abnormal plasma cell clones in bone marrow of primary systemic light chain amyloidosis patients.CHINESE MEDICAL JOURNAL,127(15),2765-2770.|
|MLA||Hu Yang,et al."Immunophenotypic analysis of abnormal plasma cell clones in bone marrow of primary systemic light chain amyloidosis patients".CHINESE MEDICAL JOURNAL 127.15(2014):2765-2770.|