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学科主题: 临床医学
题名:
Low expression of glycoprotein subunit 130 in ejaculated spermatozoa from asthenozoospermic men
作者: Cai, Zhi-Ming; Gui, Yao-Ting; Guo, Xin; Yu, Jing; Guo, Lian-Dian; Zhang, Li-Bing; Wang, He; Yu, Jie
关键词: interleukin-6 ; human spermatozoa ; motility
刊名: JOURNAL OF ANDROLOGY
发表日期: 2006-09-01
DOI: 10.2164/jandrol.106.000562
卷: 27, 期:5, 页:645-652
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Andrology
研究领域[WOS]: Endocrinology & Metabolism
关键词[WOS]: SPERM-MOTILITY ; SEMINAL PLASMA ; GENE-EXPRESSION ; SERTOLI-CELLS ; INFERTILE MEN ; FERTILE MEN ; INTERLEUKIN-6 ; RECEPTOR ; RNA ; SPERMATOGENESIS
英文摘要:

Previous studies showed that interleukin-6 (IL-6) was expressed in human Leydig and Sertoli cells and that it inhibited sperm motility. The aim of this study was to compare the expression of IL-6, IL-6R, and GP130 in ejaculated spermatozoa between normozoospermic and asthenozoospermic men. Human spermatozoa in the semen were purified by Percoll gradient technique to separate the seminal plasma and other round cells. RT-PCR, immunocytochemistry, and Western blot were used to detect the expression of IL-6, IL-6R, and GP130 in spermatozoa. With RTPCR, only GP130 mRNA but not IL-6 and IL-6R mRNA was expressed in human ejaculated spermatozoa. The expression of GP130 mRNA was significantly lower in asthenozoospermic men than in normozoospermic men. The protein expression of GP130 was further confirmed by both immunocytochemistry and Western blot. Again, GP130 protein levels were significantly lower in asthenozoospermic men than in normozoospermic men. The results suggested that the decreased expression of GP130 in ejaculated spermatozoa could be associated with low sperm motility in asthenozoospermic men.

语种: 英语
WOS记录号: WOS:000240608200005
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/51032
Appears in Collections:北京大学深圳医院_生殖医学科_期刊论文

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作者单位: Peking Univ, Shenzhen Hosp, Ctr Reprod Med, Lab Male Reprod Med, Shenzhen 518036, Peoples R China

Recommended Citation:
Cai, Zhi-Ming,Gui, Yao-Ting,Guo, Xin,et al. Low expression of glycoprotein subunit 130 in ejaculated spermatozoa from asthenozoospermic men[J]. JOURNAL OF ANDROLOGY,2006,27(5):645-652.
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