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Low expression of glycoprotein subunit 130 in ejaculated spermatozoa from asthenozoospermic men
Cai, Zhi-Ming; Gui, Yao-Ting; Guo, Xin; Yu, Jing; Guo, Lian-Dian; Zhang, Li-Bing; Wang, He; Yu, Jie
关键词Interleukin-6 Human Spermatozoa Motility
刊名JOURNAL OF ANDROLOGY
2006-09-01
DOI10.2164/jandrol.106.000562
27期:5页:645-652
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Andrology
研究领域[WOS]Endocrinology & Metabolism
关键词[WOS]SPERM-MOTILITY ; SEMINAL PLASMA ; GENE-EXPRESSION ; SERTOLI-CELLS ; INFERTILE MEN ; FERTILE MEN ; INTERLEUKIN-6 ; RECEPTOR ; RNA ; SPERMATOGENESIS
英文摘要

Previous studies showed that interleukin-6 (IL-6) was expressed in human Leydig and Sertoli cells and that it inhibited sperm motility. The aim of this study was to compare the expression of IL-6, IL-6R, and GP130 in ejaculated spermatozoa between normozoospermic and asthenozoospermic men. Human spermatozoa in the semen were purified by Percoll gradient technique to separate the seminal plasma and other round cells. RT-PCR, immunocytochemistry, and Western blot were used to detect the expression of IL-6, IL-6R, and GP130 in spermatozoa. With RTPCR, only GP130 mRNA but not IL-6 and IL-6R mRNA was expressed in human ejaculated spermatozoa. The expression of GP130 mRNA was significantly lower in asthenozoospermic men than in normozoospermic men. The protein expression of GP130 was further confirmed by both immunocytochemistry and Western blot. Again, GP130 protein levels were significantly lower in asthenozoospermic men than in normozoospermic men. The results suggested that the decreased expression of GP130 in ejaculated spermatozoa could be associated with low sperm motility in asthenozoospermic men.

语种英语
WOS记录号WOS:000240608200005
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被引频次:8[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/51032
专题北京大学深圳医院_生殖医学科
北京大学第一临床医学院_医学影像科
作者单位Peking Univ, Shenzhen Hosp, Ctr Reprod Med, Lab Male Reprod Med, Shenzhen 518036, Peoples R China
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Cai, Zhi-Ming,Gui, Yao-Ting,Guo, Xin,et al. Low expression of glycoprotein subunit 130 in ejaculated spermatozoa from asthenozoospermic men[J]. JOURNAL OF ANDROLOGY,2006,27(5):645-652.
APA Cai, Zhi-Ming.,Gui, Yao-Ting.,Guo, Xin.,Yu, Jing.,Guo, Lian-Dian.,...&Yu, Jie.(2006).Low expression of glycoprotein subunit 130 in ejaculated spermatozoa from asthenozoospermic men.JOURNAL OF ANDROLOGY,27(5),645-652.
MLA Cai, Zhi-Ming,et al."Low expression of glycoprotein subunit 130 in ejaculated spermatozoa from asthenozoospermic men".JOURNAL OF ANDROLOGY 27.5(2006):645-652.
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