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学科主题基础医学
Identification of tumor metastasis-related gene TMSG-1 by mRNA differential display
Ma, CS; Liu, YX; Zheng, J; Fang, WG; You, JF; Wang, JL; Cui, XL; Wu, BQ
关键词prostate cancer cancer metastasis mRNA differential display
刊名SCIENCE IN CHINA SERIES C-LIFE SCIENCES
2002-10-01
45期:5页:553-U1
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Biology
研究领域[WOS]Life Sciences & Biomedicine - Other Topics
关键词[WOS]SUPPRESSOR GENE
英文摘要

To investigate genes involved in cancer metastasis, mRNA differential display was used to compare the levels of gene expression of two cancer sublines derived from prostate carcinoma cell PC-3M that had different metastatic potentials. The differentially expressed genes were confirmed by Northern blot, and sequenced. The full-length cDNA of a tumor metastasis suppressor gene (TMSG-1) was obtained by using EST assembling and verified by RT-PCR and sequencing. The results showed that expression levels of TMSG-1 were lower in the highly metastatic cell line 1E8, compared with the non-metastatic cell line 2B4. The difference was significant. Full-length cDNA of TMSG-1 was about 2 kb, containing an open reading frame that encoded a protein of 230 amino acids. GenBank Blastn showed no marked homology with known genes. The functional prediction of amino acids sequence encoded by TMSG-1 gene indicated TMSG-1 protein was transmembrane protein, with 3 transmembrane domains, 3 putative protein kinase phosphorylation sites, 2 casein kinase I I phosphorylation sites and 1 N-myristoylation site. The pattern of TMSG-1 expression in 6 types of human tumor tissues indicated levels of transcripts were the highest in prostate carcinoma. TMSG-1 had lower expression in metastases of lung carcinoma compared to primary lung carcinoma. Similarly the expression levels were higher in well-differentiated colon carcinoma than that in poorly differentiated colon carcinoma. TMSG-1 could also be detected in breast, ovarian, and pancreatic carcinoma. In 9 samples of primary gastric carcinoma tissues, RT-PCR and densitometric analysis demonstrated TMSG-1 expression levels in samples with lymph node metastases; had a decreased tendency, compared to those without lymph node metastases. The difference was significant by student′s t test (P<0.05). These results indicated TMSG-1 expression levels were inversely correlated with tumor metastatic potential.

语种英语
WOS记录号WOS:000178162600012
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被引频次:11[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
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条目标识符http://ir.bjmu.edu.cn/handle/400002259/51033
专题北京大学基础医学院_病理学系
北京大学医学部管理机构_主任办公室党委办公室
北京大学医学图书馆_流通阅览部
北京大学口腔医学院_儿童口腔科
作者单位Peking Univ, Hlth Sci Ctr, Dept Pathol, Beijing 100083, Peoples R China
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Ma, CS,Liu, YX,Zheng, J,et al. Identification of tumor metastasis-related gene TMSG-1 by mRNA differential display[J]. SCIENCE IN CHINA SERIES C-LIFE SCIENCES,2002,45(5):553-U1.
APA Ma, CS.,Liu, YX.,Zheng, J.,Fang, WG.,You, JF.,...&Wu, BQ.(2002).Identification of tumor metastasis-related gene TMSG-1 by mRNA differential display.SCIENCE IN CHINA SERIES C-LIFE SCIENCES,45(5),553-U1.
MLA Ma, CS,et al."Identification of tumor metastasis-related gene TMSG-1 by mRNA differential display".SCIENCE IN CHINA SERIES C-LIFE SCIENCES 45.5(2002):553-U1.
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