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学科主题: 基础医学
题名:
Identification of tumor metastasis-related gene TMSG-1 by mRNA differential display
作者: Ma, CS; Liu, YX; Zheng, J; Fang, WG; You, JF; Wang, JL; Cui, XL; Wu, BQ
关键词: prostate cancer ; cancer metastasis ; mRNA differential display
刊名: SCIENCE IN CHINA SERIES C-LIFE SCIENCES
发表日期: 2002-10-01
卷: 45, 期:5, 页:553-U1
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Biology
研究领域[WOS]: Life Sciences & Biomedicine - Other Topics
关键词[WOS]: SUPPRESSOR GENE
英文摘要:

To investigate genes involved in cancer metastasis, mRNA differential display was used to compare the levels of gene expression of two cancer sublines derived from prostate carcinoma cell PC-3M that had different metastatic potentials. The differentially expressed genes were confirmed by Northern blot, and sequenced. The full-length cDNA of a tumor metastasis suppressor gene (TMSG-1) was obtained by using EST assembling and verified by RT-PCR and sequencing. The results showed that expression levels of TMSG-1 were lower in the highly metastatic cell line 1E8, compared with the non-metastatic cell line 2B4. The difference was significant. Full-length cDNA of TMSG-1 was about 2 kb, containing an open reading frame that encoded a protein of 230 amino acids. GenBank Blastn showed no marked homology with known genes. The functional prediction of amino acids sequence encoded by TMSG-1 gene indicated TMSG-1 protein was transmembrane protein, with 3 transmembrane domains, 3 putative protein kinase phosphorylation sites, 2 casein kinase I I phosphorylation sites and 1 N-myristoylation site. The pattern of TMSG-1 expression in 6 types of human tumor tissues indicated levels of transcripts were the highest in prostate carcinoma. TMSG-1 had lower expression in metastases of lung carcinoma compared to primary lung carcinoma. Similarly the expression levels were higher in well-differentiated colon carcinoma than that in poorly differentiated colon carcinoma. TMSG-1 could also be detected in breast, ovarian, and pancreatic carcinoma. In 9 samples of primary gastric carcinoma tissues, RT-PCR and densitometric analysis demonstrated TMSG-1 expression levels in samples with lymph node metastases; had a decreased tendency, compared to those without lymph node metastases. The difference was significant by student′s t test (P<0.05). These results indicated TMSG-1 expression levels were inversely correlated with tumor metastatic potential.

语种: 英语
WOS记录号: WOS:000178162600012
Citation statistics:
内容类型: 期刊论文
版本: 出版稿
URI标识: http://ir.bjmu.edu.cn/handle/400002259/51033
Appears in Collections:基础医学院_病理学系_期刊论文

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作者单位: Peking Univ, Hlth Sci Ctr, Dept Pathol, Beijing 100083, Peoples R China

Recommended Citation:
Ma, CS,Liu, YX,Zheng, J,et al. Identification of tumor metastasis-related gene TMSG-1 by mRNA differential display[J]. SCIENCE IN CHINA SERIES C-LIFE SCIENCES,2002,45(5):553-U1.
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