IR@PKUHSC  > 北京大学第二临床医学院  > 妇科肿瘤中心
学科主题临床医学
Establishment and characterization of a human ovarian sarcomatoid carcinoma cell line BUPH : OVSC
Guo, H; Feng, J; Liu, G; Cui, H; Ye, X; Yao, Y; Fu, T
关键词Cell Line Ovarian Tumor Primary Culture Sarcomatoid Carcinoma
刊名INTERNATIONAL JOURNAL OF GYNECOLOGICAL CANCER
2005-09-01
15期:5页:856-865
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Oncology ; Obstetrics & Gynecology
研究领域[WOS]Oncology ; Obstetrics & Gynecology
关键词[WOS]ANAPLASTIC CARCINOMA ; MURAL NODULES ; MUCINOUS CYSTADENOCARCINOMA ; TUMOR ; CYSTADENOMA ; CULTURE
英文摘要

We first established a human ovarian sarcomatoid carcinoma cell line designated BUPH:OVSC from primary culture. The specimen was derived from the mural nodule in an ovarian mucinous tumor and cultured in vitro. To date, the cell line has been maintained for over 100 passages. Its biologic characteristics were studied by light and electron microscopy, which revealed spindle-shaped or polygonal cells with a doubling time of 39.5 h. The agglutination test of BUPH: OVSC was positive, and cell colonies were formed in soft agar. Chromosome analysis revealed its karyotype to be a pseudodiploidy. One X chromosome deletion and chromosome 20 addition were detected, and aberrant chromosomes t (1q; 12q) and 14p(+) were its chromosome markers. BUPH: OVSC was tumorigenic in nude mice. Hematoxylin and eosin staining of transplanted tumors showed that the cells were morphologically sarcomatoid. However, the transmission electron microscopic observation exposed its epithelial origin. The cell line coexpresses cytokeratin and vimentin. It dose not appear to express estrogen and progesterone receptors or the CA125 tumor marker. Alcian blue/periodic acid-Schiff staining indicates that the cells could secrete acid mucopolysaccharide. In conclusion, BUPH: OVSC displays unique cellular properties, which make it a useful model for the study of human ovarian sarcomatoid carcinomas.

语种英语
WOS记录号WOS:000231798800023
Citation statistics
Cited Times:2[WOS]   [WOS Record]     [Related Records in WOS]
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/51042
Collection北京大学第二临床医学院_妇科肿瘤中心
作者单位Peking Univ, Peoples Hosp, Gynecol Oncol Ctr, Beijing 100044, Peoples R China
Recommended Citation
GB/T 7714
Guo, H,Feng, J,Liu, G,et al. Establishment and characterization of a human ovarian sarcomatoid carcinoma cell line BUPH : OVSC[J]. INTERNATIONAL JOURNAL OF GYNECOLOGICAL CANCER,2005,15(5):856-865.
APA Guo, H.,Feng, J.,Liu, G.,Cui, H.,Ye, X.,...&Fu, T.(2005).Establishment and characterization of a human ovarian sarcomatoid carcinoma cell line BUPH : OVSC.INTERNATIONAL JOURNAL OF GYNECOLOGICAL CANCER,15(5),856-865.
MLA Guo, H,et al."Establishment and characterization of a human ovarian sarcomatoid carcinoma cell line BUPH : OVSC".INTERNATIONAL JOURNAL OF GYNECOLOGICAL CANCER 15.5(2005):856-865.
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