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学科主题: 临床医学
题名:
Cytoplasmic p21 is responsible for paclitaxel resistance in ovarian cancer A2780 cells
作者: Xia, X.1,2,3; Ji, T.3; Liu, R.3; Weng, Y.3; Fang, Y.3; Wang, Z.2; Xu, H.4
关键词: Cytoplasmic p21 ; PTX resistance ; Drug resistance ; Ovarian cancer ; Akt2
刊名: EUROPEAN JOURNAL OF GYNAECOLOGICAL ONCOLOGY
发表日期: 2015
卷: 36, 期:6, 页:662-666
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Oncology ; Obstetrics & Gynecology
研究领域[WOS]: Oncology ; Obstetrics & Gynecology
关键词[WOS]: CISPLATIN ; OVEREXPRESSION ; LOCALIZATION ; CHEMOTHERAPY ; SENSITIVITY ; ACTIVATION ; EXPRESSION ; PREDICTOR ; APOPTOSIS ; PATHWAY
英文摘要:

Purpose: P21 which bound to cyclin-dependent kinase complexes was originally described as a suppressor of cancer cell proliferation, while many recent studies have shown p21, when accumulated in the cell cytoplasm, could promote tumor progression. This study was conducted to investigate the role of p21 in the paclitaxel (PTX) resistance of ovarian cancer. Materials and Methods: Regulation of cytoplasmic p21 was performed through transfection of Akt2 constitutively active vector, Akt2 shRNA and p21 siRNA in the ovarian cancer cell line A2780. Akt2, p-Akt, and p21 expression were examined by Western blot and cell apoptosis rates were assessed by flow cytometry after treatment with PTX. Results: Induction of p21 translocation into the cytoplasm via constitutively active Akt2 transfection in A2780 enhanced the resistance to PTX, while inhibition of p21 translocation into the cytoplasm via Akt2 shRNA transfection in A2780 cells significantly increased PTX treatment sensitivity. Furthermore, knockdown of cytoplasmic p21 by direct p21 siRNA transfection in Akt2 overexpressed A2780 cells notably increased PTX-induced apoptosis. Conclusion: Cytoplasmic p21 may represent a potential therapeutic target for ovarian tumors that are resistant to PTX treatment.

语种: 英语
所属项目编号: 81101971 ; 81471508 ; 81001151 ; B2011295 ; S2011040006012 ; 20110422597 ; 201002006
项目资助者: National Science Foundation ; Guangdong Natural Science Foundation ; Shenzhen Scientific Program
WOS记录号: WOS:000365830600007
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/51047
Appears in Collections:北京大学深圳医院_期刊论文

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作者单位: 1.Peking Univ, Shenzhen Hosp, Dept Reprod Ctr, Shenzhen, Peoples R China
2.Nanshan Peoples Hosp, Guangdong Med Coll, Dept Gynecol & Obstet, Shenzhen, Peoples R China
3.Huazhong Univ Sci & Technol, Tongji Hosp, Tongji Med Coll, Canc Biol Res Ctr, Wuhan 430074, Peoples R China
4.Jinan Univ, Clin Med Coll 2, Shenzhen Peoples Hosp, Dept Gynecol & Obstet, Shenzhen, Peoples R China

Recommended Citation:
Xia, X.,Ji, T.,Liu, R.,et al. Cytoplasmic p21 is responsible for paclitaxel resistance in ovarian cancer A2780 cells[J]. EUROPEAN JOURNAL OF GYNAECOLOGICAL ONCOLOGY,2015,36(6):662-666.
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