IR@PKUHSC  > 北京大学深圳医院
学科主题临床医学
Cytoplasmic p21 is responsible for paclitaxel resistance in ovarian cancer A2780 cells
Xia, X.1,2,3; Ji, T.3; Liu, R.3; Weng, Y.3; Fang, Y.3; Wang, Z.2; Xu, H.4
关键词Cytoplasmic P21 Ptx Resistance Drug Resistance Ovarian Cancer Akt2
刊名EUROPEAN JOURNAL OF GYNAECOLOGICAL ONCOLOGY
2015
36期:6页:662-666
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Oncology ; Obstetrics & Gynecology
研究领域[WOS]Oncology ; Obstetrics & Gynecology
关键词[WOS]CISPLATIN ; OVEREXPRESSION ; LOCALIZATION ; CHEMOTHERAPY ; SENSITIVITY ; ACTIVATION ; EXPRESSION ; PREDICTOR ; APOPTOSIS ; PATHWAY
英文摘要

Purpose: P21 which bound to cyclin-dependent kinase complexes was originally described as a suppressor of cancer cell proliferation, while many recent studies have shown p21, when accumulated in the cell cytoplasm, could promote tumor progression. This study was conducted to investigate the role of p21 in the paclitaxel (PTX) resistance of ovarian cancer. Materials and Methods: Regulation of cytoplasmic p21 was performed through transfection of Akt2 constitutively active vector, Akt2 shRNA and p21 siRNA in the ovarian cancer cell line A2780. Akt2, p-Akt, and p21 expression were examined by Western blot and cell apoptosis rates were assessed by flow cytometry after treatment with PTX. Results: Induction of p21 translocation into the cytoplasm via constitutively active Akt2 transfection in A2780 enhanced the resistance to PTX, while inhibition of p21 translocation into the cytoplasm via Akt2 shRNA transfection in A2780 cells significantly increased PTX treatment sensitivity. Furthermore, knockdown of cytoplasmic p21 by direct p21 siRNA transfection in Akt2 overexpressed A2780 cells notably increased PTX-induced apoptosis. Conclusion: Cytoplasmic p21 may represent a potential therapeutic target for ovarian tumors that are resistant to PTX treatment.

语种英语
WOS记录号WOS:000365830600007
项目编号81101971 ; 81471508 ; 81001151 ; B2011295 ; S2011040006012 ; 20110422597 ; 201002006
资助机构National Science Foundation ; Guangdong Natural Science Foundation ; Shenzhen Scientific Program
引用统计
被引频次:6[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/51047
专题北京大学深圳医院
作者单位1.Peking Univ, Shenzhen Hosp, Dept Reprod Ctr, Shenzhen, Peoples R China
2.Nanshan Peoples Hosp, Guangdong Med Coll, Dept Gynecol & Obstet, Shenzhen, Peoples R China
3.Huazhong Univ Sci & Technol, Tongji Hosp, Tongji Med Coll, Canc Biol Res Ctr, Wuhan 430074, Peoples R China
4.Jinan Univ, Clin Med Coll 2, Shenzhen Peoples Hosp, Dept Gynecol & Obstet, Shenzhen, Peoples R China
推荐引用方式
GB/T 7714
Xia, X.,Ji, T.,Liu, R.,et al. Cytoplasmic p21 is responsible for paclitaxel resistance in ovarian cancer A2780 cells[J]. EUROPEAN JOURNAL OF GYNAECOLOGICAL ONCOLOGY,2015,36(6):662-666.
APA Xia, X..,Ji, T..,Liu, R..,Weng, Y..,Fang, Y..,...&Xu, H..(2015).Cytoplasmic p21 is responsible for paclitaxel resistance in ovarian cancer A2780 cells.EUROPEAN JOURNAL OF GYNAECOLOGICAL ONCOLOGY,36(6),662-666.
MLA Xia, X.,et al."Cytoplasmic p21 is responsible for paclitaxel resistance in ovarian cancer A2780 cells".EUROPEAN JOURNAL OF GYNAECOLOGICAL ONCOLOGY 36.6(2015):662-666.
条目包含的文件
条目无相关文件。
个性服务
推荐该条目
保存到收藏夹
查看访问统计
导出为Endnote文件
谷歌学术
谷歌学术中相似的文章
[Xia, X.]的文章
[Ji, T.]的文章
[Liu, R.]的文章
百度学术
百度学术中相似的文章
[Xia, X.]的文章
[Ji, T.]的文章
[Liu, R.]的文章
必应学术
必应学术中相似的文章
[Xia, X.]的文章
[Ji, T.]的文章
[Liu, R.]的文章
相关权益政策
暂无数据
收藏/分享
所有评论 (0)
暂无评论
 

除非特别说明,本系统中所有内容都受版权保护,并保留所有权利。