北京大学医学部机构知识库
Advanced  
IR@PKUHSC  > 北京大学医药卫生分析中心  > 期刊论文
学科主题: 公共卫生
题名:
Tc-99m-Labeled RGD-BBN Peptide for Small-Animal SPECT/CT of Lung Carcinoma
作者: Liu, Zhaofei1,2; Huang, Jinming1,2; Dong, Chengyan1,2; Cui, Liyang1,2; Jin, Xiaona3; Jia, Bing1,2; Zhu, Zhaohui3; Li, Fang3; Wang, Fan1,2
关键词: RGD-bombesin ; integrin alpha(v)beta(3) ; GRPR ; lung carcinoma ; single-photon emission computed tomography (SPECT)
刊名: MOLECULAR PHARMACEUTICS
发表日期: 2012-05-01
DOI: 10.1021/mp200661t
卷: 9, 期:5, 页:1409-1417
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Medicine, Research & Experimental ; Pharmacology & Pharmacy
研究领域[WOS]: Research & Experimental Medicine ; Pharmacology & Pharmacy
关键词[WOS]: GASTRIN-RELEASING-PEPTIDE ; BOMBESIN HETERODIMER ; TUMOR-CELLS ; CANCER ; PET ; INFLAMMATION ; F-18-FDG ; FDG ; INTEGRIN ; EXPRESSION
英文摘要:

We recently designed and synthesized a Gluc(RGDyK)-bombesin (RGD-BBN) heterodimeric peptide exhibiting a dual integrin alpha(v)beta(3) and gastrin-releasing peptide receptor (GRPR) targeting property. In this study, we investigated whether Tc-99m-labeled RGD-BBN peptide could be used for the non-invasive detection of lung carcinoma by using small-animal single-photon emission computed tomography (SPECT)/CT. RGD-BBN peptide was conjugated with 6-hydrazinonicotinyl (HYNIC) and then radiolabeled with Tc-99m using tricine and TPPTS as the coligands (TPPTS = trisodium triphenylphosphine-3,3′,3 ′′-trisulfonate). The biodistribution, planar gamma imaging, and small-animal SPECT/CT studies of Tc-99m-HYNIC(tricine)(TPPTS)-RGD-BBN (Tc-99m-RGD-BBN) were performed in C57/BL6 mice bearing Lewis lung carcinoma (LLC) or bearing both inflammation and LLC. HYNIC-RGD-BBN possessed a dual integrin alpha(v)beta(3) and GRPR binding capacity. Tc-99m-RGD-BBN was prepared with a high radiochemical purity (>98%), and it exhibited specific tumor imaging with high contrast to the contralateral background. Tc-99m-RGD-BBN was superior to F-18-FDG for distinguishing lung carcinoma from inflammation. The uptake of Tc-99m-RGD-BBN in LLC xenografts was 2.69 +/- 0.66% ID/g at 1 h postinjection (p.i.) and was decreased to 1.99 +/- 0.61% ID/g at 2 h p.i. The inflammation uptake of Tc-99m-RGD-BBN was 1.20 +/- 0.32% ID/g at 1 h and 0.56 +/- 0.17% ID/g at 2 h p.i., respectively. High pancreas uptake (25.76 +/- 5.49%ID/g and 19.56 +/- 6.78% ID/g at 1 and 2 h p.i., respectively) was also found due to the high GRPR expression of this organ. Small-animal SPECT/CT using Tc-99m-RGD-BBN can specifically detect the LLC pulmonary metastases. Our results suggested that SPECT/CT with Tc-99m-RGD-BBN would provide an effective approach for the noninvasive detection of lung cancer.

语种: 英语
所属项目编号: 81000625 ; 30930030 ; 30870728 ; 30900373 ; 81028009 ; 81125011 ; 2011CB707703 ; 2009ZX09103-733 ; 2012ZX09102301-018 ; 2011YQ030114
项目资助者: National Natural Science Foundation of China (NSFC) ; Outstanding Youth Fund ; "973" project ; Ministry of Science and Technology of China
WOS记录号: WOS:000303961100035
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/51058
Appears in Collections:北京大学医药卫生分析中心_期刊论文

Files in This Item:

There are no files associated with this item.


作者单位: 1.Peking Univ, Med Isotopes Res Ctr, Beijing 100191, Peoples R China
2.Peking Univ, Sch Basic Med Sci, Dept Radiat Med, Beijing 100191, Peoples R China
3.Beijing Union Med Coll Hosp, Dept Nucl Med, Beijing 100857, Peoples R China

Recommended Citation:
Liu, Zhaofei,Huang, Jinming,Dong, Chengyan,et al. Tc-99m-Labeled RGD-BBN Peptide for Small-Animal SPECT/CT of Lung Carcinoma[J]. MOLECULAR PHARMACEUTICS,2012,9(5):1409-1417.
Service
Recommend this item
Sava as my favorate item
Show this item's statistics
Export Endnote File
Google Scholar
Similar articles in Google Scholar
[Liu, Zhaofei]'s Articles
[Huang, Jinming]'s Articles
[Dong, Chengyan]'s Articles
CSDL cross search
Similar articles in CSDL Cross Search
[Liu, Zhaofei]‘s Articles
[Huang, Jinming]‘s Articles
[Dong, Chengyan]‘s Articles
Related Copyright Policies
Null
Social Bookmarking
Add to CiteULike Add to Connotea Add to Del.icio.us Add to Digg Add to Reddit

Items in IR are protected by copyright, with all rights reserved, unless otherwise indicated.

 

 

Valid XHTML 1.0!
Copyright © 2007-2017  北京大学医学部 - Feedback
Powered by CSpace