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学科主题: 基础医学
题名:
Ganoderma lucidum polysaccharides counteract inhibition on CD71 and FasL expression by culture supernatant of B16F10 cells upon lymphocyte activation
作者: Sun, Li-Xin1; Lin, Zhi-Bin2; Duan, Xin-Suo1; Lu, Jie1; Ge, Zhi-Hua1; Li, Min2; Xing, En-Hong1; Lan, Tian-Fei1; Jiang, Miao-Miao1; Yang, Ning1; Li, Wei-Dong2
关键词: tumor ; lymphocyte activation ; Ganoderma lucidum ; polysaccharides ; FasL ; CD71
刊名: EXPERIMENTAL AND THERAPEUTIC MEDICINE
发表日期: 2013-04-01
DOI: 10.3892/etm.2013.931
卷: 5, 期:4, 页:1117-1122
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Medicine, Research & Experimental
研究领域[WOS]: Research & Experimental Medicine
关键词[WOS]: BLOOD MONONUCLEAR-CELLS ; IN-VITRO ; DENDRITIC CELLS ; MECHANISMS ; CANCER ; LIGAND ; DEATH ; SUPPRESSION ; RECEPTOR ; PROLIFERATION
英文摘要:

Immune responses to tumor-associated antigens are often detectable in tumor-bearing hosts, but they fail to eliminate malignant cells or prevent development of metastases. Tumor cells produce factors such as interleukin-10, transforming growth factor-beta 1 and vascular endothelial growth factor (VEGF) that suppress the function of immune cells or induce apoptosis of immune cells. Culture supernatant of tumor cells may contain these immunosuppressive factors which suppress lymphocyte activation. CD71 and FasL are two important molecules that are expressed upon lymphocyte activation. Counteraction against suppression CD71 and FasL expression upon lymphocyte activation may benefit tumor control. A potential component with this effect is Ganoderma lucidum polysaccharides (Gl-PS). In this study, Gl-PS was used on lymphocytes incubating with culture supernatant of B16F10 melanoma cells (B16F10-CS) in the presence of phytohemagglutinin. Following induction with phytohemagglutinin, B16F10-CS suppressed CD71 expression in lymphocytes (as detected by immunofluorescence and flow cytometry), proliferation in lymphocytes (as detected by MTT assay), and FasL expression in lymphocytes (as detected by immunocytochemistry and western blot analysis), while Gl-PS fully or partially counteracted these suppressions. Gl-PS showed counteractive effects against suppression induced by B16F10-CS on CD71 and FasL expression upon lymphocyte activation, suggesting the potential of Gl-PS to facilitate cancer immunotherapy.

语种: 英语
WOS记录号: WOS:000321937400026
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/51070
Appears in Collections:基础医学院_药理学系_期刊论文

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作者单位: 1.Chengde Med Coll, Affiliated Hosp, Chengde 067000, Hebei, Peoples R China
2.Peking Univ, Hlth Sci Ctr, Sch Basic Med Sci, Dept Pharmacol, Beijing 100191, Peoples R China

Recommended Citation:
Sun, Li-Xin,Lin, Zhi-Bin,Duan, Xin-Suo,et al. Ganoderma lucidum polysaccharides counteract inhibition on CD71 and FasL expression by culture supernatant of B16F10 cells upon lymphocyte activation[J]. EXPERIMENTAL AND THERAPEUTIC MEDICINE,2013,5(4):1117-1122.
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